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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 838 results for: ANG

Showing: 401 - 420 of 838 results

  2. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...sure the feature you want to verify is within this range. Read some of our other blog posts for more tips... simplify the process. First, nearly all of our Sanger sequencing is done from glycerol stocks (overnight..., and not all providers are equipped to perform Sanger sequencing from glycerol stocks, so make sure to...

  3. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...are for the coaching relationship. Do you want to change jobs? Grow your professional presence? Learn a ...and make direct suggestions for improvement and change. Work with the coach to define how you will know...work with you remotely (by phone, Skype or Google Hangout) but in-person coaching is nice if you can get ...

  4. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ... loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target...CRISPR/Cas9 genome editing can result in unwanted changes at non-target sites. Means to decrease these so-called... Recognizing these issues, researchers from the Zhang lab at the Broad Institute, the Joung lab at Harvard...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    ...microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available for antibody conjugation...and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based... choice for ELISAs.  Biotin Biotin is used in a range of antibody-based assays. Biotin itself is not visible...

  6. Working with Nuclear Receptors

    Type
    Blog Post
    ... govern expression of genes involved in a broad range of reproductive, developmental, metabolic, and immune... expression and reporter plasmids and the free exchange of these vectors between laboratories. The development...Reading: Chawla, A., Repa, J. J., Evans, R. M.and Mangelsdorf, D. J. (2001). Nuclear receptors and lipid physiology...

  7. Engaging with science and society at pgEd

    Type
    Blog Post
    ...information briefs for policymakers. The rapidly changing landscape of genetics and our consistent stream...society, and an ability to talk about science in a language that can be understood by those who are not experts...communicate in very technical and jargon-filled language in academic writing and presentations. But for...

  10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...common and will stand out; however, be careful not to hang your poster too low. If it is pinned to low, some...inserted into larger circles or ovals, that are arranged in some logical pattern across the poster. This...into circular shapes rather than a square or rectangular boxes. Background and color One of the best ...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...experiment. References 1. Kutner, Robert H., Xian-Yang Zhang, and Jakob Reiser. "Production, concentration... 16836756. PubMed Central PMCID: PMC1534021. 3. Zhang, Bing, et al. "The significance of controlled conditions...

  12. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...Virus-specific concerns   In nature, some viruses are more dangerous than others, and the same holds true for types...For many researchers, viruses might be the most dangerous things they work with, and that can understandably...let you work with these valuable tools without endangering yourself or others. Happy transducing! Resources...

  13. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...the only right way, and Indecisives, who keep changing their minds and have trouble making decisions....talking about your emotions, such as frustration or anger, because your discussion will get side-tracked, ...instead of email. As you cannot see a person’s body language or sense their tone of voice in an email, there...

  14. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...AAV-containing 40% fraction is collected and buffer exchanged to remove the iodixanol and concentrate the purified...or the virus can be stored at 4 °C and buffer exchanged the next day. Refer to the AAV Iodixanol Gradient...correctly. When aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots...

  15. Pooled Library Amplifications

    Type
    Blog Post
    ...transformation amplification protocol is the Chong Zhang E. coli Genome-wide Inhibition Library. DNA and...SPLINTR Libraries (PDF, 401 KB) Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors...transcriptional activation screening, a protocol paper by the Zhang lab, and the Broad Institute’s general pDNA Library...

  16. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since the discovery...Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support...References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...overwhelming when you are presented with a sea of strangers. Try things like sitting at a table for lunch ... for myself like meet ten new people each day. Exchange business cards. When you’re done networking for... Jennifer Tsang...

  19. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...thus a heat resistant enzyme that can withstand changes in temperature. Taq was first identified in the...Annealing- The temperature is then dropped to a range in which the forward and reverse primers are stable...Quantifying low abundance targets Monitoring subtle changes in target DNA levels Multiplex PCR Amplify...

  20. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed... NgAgo/ssDNA requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells...website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for...
Showing: 401 - 420 of 838 results