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We narrowed to 963 results for: APP

Showing: 401 - 420 of 963 results

  1. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...previous post in this series described a practical approach to selecting a bright fluorescent protein. In ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets in a water/oil emulsion, (ii) ...

  2. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...systematically mutated it. Using a robotic picking approach to select cells with variant characteristics they...” vector cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest ...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...

  3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...researchers to better understand the processes that happen inside the cell,” says Pedre Pérez. Figure...construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due...

  4. Summer SciComm: Preprints

    Type
    Blog Post
    ...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)...and preprints are a great way to do that. We are happy to receive deposits of plasmids that have been described...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

  5. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...several methodologies currently available for approaching it. These include the traditional restriction...introduction.html demonstrating the principal behind the “swapping out” of ccdB with the investigator’s gene of interest...which can then be used for downstream cloning applications. While these two strains ultimately perform ...important to ensure that you choose the most appropriate method for your own experiments. Please check...

  6. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...high-throughput screening are vastly useful for mapping the combinatorial genetics that underlie complex...research questions. CombiGEM has been successfully applied to functionally characterize combinatorial gene...realization of a variety of perturbations and applications in functional genomics, cell reprogramming, ...He is particularly interested in developing and applying new technologies to interrogate and understand...

  7. A Look at Addgene's QC Process

    Type
    Blog Post
    ...mutated; elements that are difficult to sequence; approaches to confirm plasmid sequences without a reference...program called Geneious. Here, the raw reads are mapped against a reference sequence, if one was provided... determine if there are any notable QC issues. Mapping the raw NGS reads to a reference sequence can also...confirm their presence with confidence. If this approach does not resolve the identified differences, the...

  8. Engaging with science and society at pgEd

    Type
    Blog Post
    ...these tough issues is essential for building a rapport with these communities, which is in turn crucial...latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...scientist. After all, scientific research does not happen in a vacuum that is isolated from the rest of society...and who will be affected by the knowledge and applications generated from this research. At both a practical...

  9. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high...AdVs is what makes them ideal candidates for applications in oncolysis and vaccination!) In the 65 years...Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new...

  10. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...to increased intracellular calcium. It has been applied to mark active populations of neurons in vivo to...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction to AAV ...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... the end goal, it is best to work with FDA/EMA approved drugs so that your results are viable for translation... be mitigated by using low doses and running appropriate controls (Jendryka et al., 2019), they have resulted...brain and some, such as varenicline, are already approved for clinical use. Varenicline has been further...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

  12. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...need to be given some time to adapt to a testing apparatus. General stress levels of the test animal may ...the humans’ audible spectrum. The experimental apparatus should be cleaned thoroughly between animals as...excitation or inhibition upon light (or drug) application.  Genetic manipulation can also reduce expression...after injection. Therefore, it’s crucial to appropriately time the injection about two weeks before the...

  13. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...differently – happens when the antibody is produced from a new set of animals), and wrong application (different...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...

  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...organizers and moderator should have a well-planned approach to making the event interesting and engaging. ...information and remind them about the event as it approaches. Room set-up and logistics To make a really...and forth within the panel. Go with it when this happens, but get things back on track if the direction ...talk and the speaker goes over 1 hour, watch what happens to the attention of the audience – phones come ...

  15. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...working with mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus...have capacities of 8–12 kb, while AAV only has approximately 4.7 kb — which means that when using Cas9, you... Platforming AAV Interest in interrogating and mapping the genetic landscape of tissue-specific diseases...Performing In Vivo CRISPR Screens Using the FITS Approach Additional resources at addgene.org CRISPR pooled...

  16. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... per year, or approximately 104 plasmids shipped per working day, including appropriate MTAs and customs...of CRISPR tools has grown steadily, gaining approximately 2,000 plasmids/year from 2017 to 2023. This ... joining the Addgene CRISPR community, with approximately 100 new CRISPR depositors each year.    ...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... experimental, biotechnological, and medical applications. Researches can visualize proteins in vivo by...protein you need   Your plans for downstream applications In this blog post we will summarize some of...proteins as bacterial cells are not equipped to add appropriate post-translational modifications. In addition...acids that are useful in different downstream applications. Cell-free expression systems however, are expensive...

  18. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...order to correct for this, compensation has to be applied to “subtract” this spillover and correctly distinguish...designing your panel. If you have two highly overlapping cellular markers, it is important to pair them...have a similarity index of 0.87; therefore, two overlapping cellular markers should not be paired with these...index of 0, which would be a better choice for overlapping markers. Compensation/reference control(s) Compensation...

  19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...have gotten a lot of press for being a promising approach for treatment or prophylaxis of COVID-19. Researchers...nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses...BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, ...single domain antibodies for the conformational trapping of membrane proteins. eLife 7: . https://doi.org...

  20. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...months, and we got one submission. I was a bit disappointed in the lack of participation, but I decided ... passed these dinosaurs every day. They made me happy. Grad school can provide plenty of negativity, so... so I volunteered at a place that made me happy. I started volunteering in the “Dinosphere” almost every...was worried about becoming one of those angry, disappointed graduate students because I wasn’t sure what...
Showing: 401 - 420 of 963 results