... are here to discuss the ins and outs of both approaches. Screens and selections The common goals The...lot in common. In fact, selections are often inappropriately categorized as screens! Why the confusion? ...details - and that’s where the differences in these approaches can be found. As an example, say you are considering...require there to be a culture condition that can apply enough pressure to select for variants of interest...

...small, avoidable mistake. Or worse, when you're trapped in a pattern of small errors that significantly...of ways you could change your variables - your approach, your workflow, or your physical setup - to prevent...workflow can have a huge impact. Test different approaches, like whether it's better for you to use cold...resistant to. Just like any other experimental approach, use small, incremental changes to understand ...

... with an intuitive user interface.   The team approached the issue from multiple angles: the overall user...of information so we were able to fine tune our approach much more than if we were dealing with general...” variations and synonyms of the same term that appear in our data into a single filter value. For example...our search engine to be so specialized that it appears to “understand” biology.  We hope you will give...

...review system. Peer review in traditional journals happens before the manuscript is accepted for publication...at the same time. Take your time picking the appropriate journal and explain in your cover letter why ...why you think your manuscript is appropriate for that journal. Argue with an editor or reviewer over their...ignored their comments, they will not be inclined to approve your paper. Rush your submission or revision. A...

...with the same genetic background and do an apples-to-apples comparison of all those mutations. If you ...take four plasmids out of the library in the appropriate combination and do a restriction enzyme digest...different genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare...

...systematically mutated it. Using a robotic picking approach to select cells with variant characteristics they...” vector cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest ...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...

...high-throughput screening are vastly useful for mapping the combinatorial genetics that underlie complex...research questions. CombiGEM has been successfully applied to functionally characterize combinatorial gene...realization of a variety of perturbations and applications in functional genomics, cell reprogramming, ...He is particularly interested in developing and applying new technologies to interrogate and understand...

...several methodologies currently available for approaching it. These include the traditional restriction...introduction.html demonstrating the principal behind the “swapping out” of ccdB with the investigator’s gene of interest...which can then be used for downstream cloning applications. While these two strains ultimately perform ...important to ensure that you choose the most appropriate method for your own experiments. Please check...

...previous post in this series described a practical approach to selecting a bright fluorescent protein. In ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets in a water/oil emulsion, (ii) ...

...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, Twitter...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)...and preprints are a great way to do that. We are happy to receive deposits of plasmids that have been described...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...researchers to better understand the processes that happen inside the cell,” says Pedre Pérez. Figure...construction of the dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due...

...these tough issues is essential for building a rapport with these communities, which is in turn crucial...latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...scientist. After all, scientific research does not happen in a vacuum that is isolated from the rest of society...and who will be affected by the knowledge and applications generated from this research. At both a practical...

...Additional red sensors based on mRuby (jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods...to increased intracellular calcium. It has been applied to mark active populations of neurons in vivo to...expression and downstream monitoring. This targeted approach allows scientists to focus on specific cell populations...Vectors 101: Biosensors Viral Vectors 101: Viral Applications Viral Vectors 101: An Introduction to AAV ...

... the end goal, it is best to work with FDA/EMA approved drugs so that your results are viable for translation... be mitigated by using low doses and running appropriate controls (Jendryka et al., 2019), they have resulted...brain and some, such as varenicline, are already approved for clinical use. Varenicline has been further...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

...mutated; elements that are difficult to sequence; approaches to confirm plasmid sequences without a reference...program called Geneious. Here, the raw reads are mapped against a reference sequence, if one was provided... determine if there are any notable QC issues. Mapping the raw NGS reads to a reference sequence can also...confirm their presence with confidence. If this approach does not resolve the identified differences, the...

...vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high...AdVs is what makes them ideal candidates for applications in oncolysis and vaccination!) In the 65 years...Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new...

...need to be given some time to adapt to a testing apparatus. General stress levels of the test animal may ...the humans’ audible spectrum. The experimental apparatus should be cleaned thoroughly between animals as...excitation or inhibition upon light (or drug) application.  Genetic manipulation can also reduce expression...after injection. Therefore, it’s crucial to appropriately time the injection about two weeks before the...

...differently – happens when the antibody is produced from a new set of animals), and wrong application (different...the best, or even which one will work for your application? Like many scientists, I have spent hours sifting...differently over time), compatible with the desired applications (such as immunohistochemistry (IHC) or western...

...organizers and moderator should have a well-planned approach to making the event interesting and engaging. ...information and remind them about the event as it approaches. Room set-up and logistics To make a really...and forth within the panel. Go with it when this happens, but get things back on track if the direction ...talk and the speaker goes over 1 hour, watch what happens to the attention of the audience – phones come ...

...order to correct for this, compensation has to be applied to “subtract” this spillover and correctly distinguish...designing your panel. If you have two highly overlapping cellular markers, it is important to pair them...have a similarity index of 0.87; therefore, two overlapping cellular markers should not be paired with these...index of 0, which would be a better choice for overlapping markers. Compensation/reference control(s) Compensation...