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We narrowed to 710 results for: BASE;

Showing: 401 - 420 of 710 results

  1. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in this blog..."The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression...

  2. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...money. They also usually have a good event-planning base and will know how to reserve space, time events,...I think many of the students learned something. Based on the initial guidelines I received from the teachers...

  3. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...allowing for in vivo biotinylation through a plasmid-based biotin ligase. Biotinylated dCas9, with bound DNA...genomic region is another example of the many tools based on targeting of a genomic locus through a specific...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...consistent development and improvement of CRISPR-based technologies over the past decade. CRISPR screens... fact supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...arises here. Notwithstanding access restrictions based on permissions, however, in some cases it would ...provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged...

  9. Tips for arabidopsis transformation

    Type
    Blog Post
    ...additionally allow for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and...inflorescence (the flowering portion of the plant) near the base of the stem. This buys you a few days until new ...

  10. Viral Production at Addgene

    Type
    Blog Post
    ...determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity Purity of...Schematic of the barcode matching system and PCR-based confirmation of transfer plasmids used in Addgene...

  11. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...less photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases...specific antibody binding sites and primary antibody-based detection, smFPs create much brighter signals for...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...1990s, researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus...actively dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, ...

  13. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...conformational changes, cleavage activity using FRET-based biosensors, and interactions between DNA and proteins...limited to a single measurement. This method is based on the fact that the donor is quenched when FRET...

  14. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target genes...Promoter specificity Aside from choosing a promoter based on type of RNA transcript, you will also need to...

  16. Editor's Choice, July 2016

    Type
    Blog Post
    ...Tool for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo...

  19. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial antibiotic...commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include information about...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... PAM requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities...toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo ...acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells...
Showing: 401 - 420 of 710 results