We narrowed to 802 results for: Clu
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TypeBlog Post...pillars for antibody validation. The 5 pillars include genetic strategies that test expression in knock-out...conditions. Be prepared to troubleshoot and be sure to include proper positive and negative controls as you get...
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Addgene: The First Twenty Years
TypeBlog Post...we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think...growing library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide... -
RNA Extraction Without A Kit
TypeBlog Post...proteins that are found in and on everything, including your skin. Here are some general tips for working...degrade RNA. Keep a clean work area, which may include spraying your bench down with a product to get ... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...recognized for its biotechnological applications including conversion of CO2 into water and electrical energy... in cyanobacteria. This kit contains 96 parts including terminators and promoters, CRISPRi parts, selectable... -
To Each HIS Own
TypeBlog Post...Bornhorst 2000). To prevent this, wash steps often include low concentrations of imidazole. The concentration... C-terminus and how many histidine residues to include. Placement As far as placement goes, there is ... -
Human Germline Editing Using CRISPR
TypeBlog Post...published, a bioRxiv preprint that questions the conclusion of inter-homologue recombination was released...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this paper... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...photobleaching rate is not linearly related to power, the conclusions reached at high power may not translate to real...cells at a power used for live-cell imaging. To conclude, the most photostable fluorescent protein can ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...proteins potently inhibit type II CRISPR systems including the popular S. pyogenes Cas9 (Rauch et al., 2017... the resulting optogenetic plasmid tools which includes plasmids encoding a fusion between cPYP and tgRFP... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...ahead of most companies in creating a welcoming, inclusive workplace for everyone. We had Unbiasing Training...movement. We are carefully attentive to genuine inclusion, but not worried because we are ahead of the curve... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...combinatorial expression of other genetic elements including transcription factors (Cheng et al., 2014) and... with effective gRNAs are publicly available, including those found at Addgene! Figure 1: Strategy... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...your expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save...plasmid from its predecessor. Important features may include cloning junctions (where most sequence assembly... -
The Future of Research Symposium Boston 2015
TypeBlog Post...sites around the USA undertaking BEST programs, including local schools in Boston: the Boston University...market. Her many notable contributions in this area include publishing the book ‘How Economics Shapes Science... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post...for a successful grant application. It typically includes overview information, key dates, funding description...deliver outsider opinions, and reveal items to include for the next application. Although some comments... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test... negative control to a sample with the primary included to confirm that the signal is from the primary... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...local gene expression, confounding any potential conclusions about the role of methylation (Hrit et al. 2018... many effects independent of dCas9 targeting, including modifying other sites in the genome and directly... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. These are ...are wells on the same ELISA plate that include known concentrations of your protein of interest. The optical... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...plasmids! This category includes genes that help bacteria invade hosts, including attaching to host cells... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...of the Retroviridae or Retrovirus family, which includes the gamma-retrovirus and lentivirus genera, have... field of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, ... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...Arg-mutant proteins in living baker’s yeast cells, including Arg-mutant yeast homologs of human proteins that...frequently found in genetic testing for syndromes that include developmental delay, result in loss of function... -
CRISPR in the Clinic
TypeBlog Post...destroy. One treatment currently in clinical trials includes Cas3 (the shredder member of the Cas family) as... Other treatments currently in clinical trials include, but are not limited to, ones targeting muscular...