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  2. To Each HIS Own

    Type
    Blog Post
    ...Bornhorst 2000). To prevent this, wash steps often include low concentrations of imidazole. The concentration... C-terminus and how many histidine residues to include.  Placement As far as placement goes, there is ...

  3. Summer SciComm: Preprints

    Type
    Blog Post
    ...journal for formal peer review. Some organizations, including some journals, also support open peer review or...community significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in ...

  5. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...ahead of most companies in creating a welcoming, inclusive workplace for everyone. We had Unbiasing Training...movement. We are carefully attentive to genuine inclusion, but not worried because we are ahead of the curve...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...published, a bioRxiv preprint that questions the conclusion of inter-homologue recombination was released...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this paper...

  7. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...photobleaching rate is not linearly related to power, the conclusions reached at high power may not translate to real...cells at a power used for live-cell imaging. To conclude, the most photostable fluorescent protein can ...

  9. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...your expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save...plasmid from its predecessor. Important features may include cloning junctions (where most sequence assembly...

  10. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...sites around the USA undertaking BEST programs, including local schools in Boston: the Boston University...market. Her many notable contributions in this area include publishing the book ‘How Economics Shapes Science...

  11. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...for a successful grant application. It typically includes overview information, key dates, funding description...deliver outsider opinions, and reveal items to include for the next application. Although some comments...

  14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. These are ...are wells on the same ELISA plate that include known concentrations of your protein of interest. The optical...

  15. CRISPR in the Clinic

    Type
    Blog Post
    ...destroy. One treatment currently in clinical trials includes Cas3 (the shredder member of the Cas family) as... Other treatments currently in clinical trials include, but are not limited to, ones targeting muscular...

  16. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test... negative control to a sample with the primary included to confirm that the signal is from the primary...

  17. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

  19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...order to see an effect. Hemizygous mice can be included in breeding strategies identical to those used...with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan, or infertility...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    ...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...published in a paper, but positive outcomes can include poster presentations, talks about your work, or...
Showing: 401 - 420 of 793 results