Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,145 results for: Gene

Showing: 401 - 420 of 1145 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...-invasive systemic gene delivery in non-human primate (NHP) brains.  Systemic gene delivery capsids previously...Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific ...Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain....plasmid here! Chen, X., et al. (2023). Functional gene delivery to and across brain vasculature of systemic...past decade, primary cells remain difficult to genetically edit due to toxicity from electroporation or ... under CC-BY license.   The Miyawaki lab generated a series of StayGold mutants and identified a ...mentioning that Kiryl Piatkevich and colleagues generated yet another monomeric StayGold variant (Piatkevich...

  2. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...these expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...matters is the number of hits to the same sequence (gene). In a clean sample, there are typically fewer than... However, a large number of hits to one sequence/gene - we set our threshold at >100 hits - immediately...example, AAV-68544 contained 220 hits to the CHRM4 gene. When contamination is suspected, we proceed to...Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum...purity. But Addgene is also conducting more rigorous quality control, including next generation sequencing... for transgene expression. Once packaged in AAV, this pre-recombined DNA could lead to transgene expression...

  3. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the nucleus...easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have ...The MoClo Plant Kit makes it really easy to clone genes for expression in plant cells. Sylvester and I chose... Now there are many, so if you want to make a 10-gene construct, you don’t have to worry about repeating...constructs to allow people to, for example, knock out a gene with simultaneous complementation, with a knockout...Marillonnet share with the scientific community via Addgene, the struggles of plant scientists and how they...Everybody's favorite parts for plant synthetic biology Addgene: Tell me about the Golden Gate Modular Cloning ...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...from that of the gene of interest, or expressed from the same transcript as the gene of interest, but ...visualize when and where the gene is expressed. Click here to view Addgene’s collection of empty backbones... a wide number of functions, including: tagging genes for elucidating their expression or localization...promoter of interest can be used to effectively monitor gene expression from that promoter in a given cell type...more about optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking...and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics: Scientists can use...implantation and generation of transgenic mice. Purification: GFP can be used as a general epitope tag for...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...dealing with a defective gene, Xm, the sequence of which is identical to the correct gene, Xw, except for a ...Adenine base editors (ABE) What if you have another gene Y, which has a defective allele Ym and you need ...first created a defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). ...transformed it into E. coli harboring the defective CamR gene. Screening for surviving colonies and subsequent...system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables... slim chance of generating indels with base editors, keep in mind that the generation of indel sometimes...biology Resources on Addgene.org Find all CRISPR plasmids and resources at Addgene Find CRISPR base editors...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017...fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first eukaryote (S. cerevisiae... new at all, but it’s not as well-known in the general research community. Phillips Petroleum Company...release the K. phaffii expression system to the general research community in the 1990s when they abandoned...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

  7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ... for gene expression, turning transcription on and off by binding proximal to their target genes. Since...Since gene expression patterns determine everything from stem cell differentiation fate to tumor suppression...from the library to further investigate specific genes and TF isoforms. Due to the size of the collection...use to generate the relevant disease model. Accurate modeling of a disease depends on both genetic and expression... not yet available, just ask an Addgene scientist at help@addgene.org! References and Resources  References...scientific community. Recently, the Zhang lab generated a TF library (aka the MORF Collection) and an ...isoforms transcribed from these sites. The Zhang lab generated a comprehensive library of each unique TF open...

  8. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ... popular tools for genetic screens, enabling genome-wide interrogation of the genes required for cellular...that constitutively express Cas9 and screen for genes that affect Th2 differentiation and activation, ...inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the researchers explore many...-LPs and accelerate the discovery of additional genes and small-molecule probes to aid in the understanding...transduction, now enabling forward genetic screens in cells with few genetic tools. Find the mouse CRISPR ...plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed PMID: 31451697. Genetically encoded ...responsible for a phenotype can be identified by next-generation sequencing of the genomic DNA. However, some ...

  9. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...ampicillin resistance gene, you’ll find the full name of the gene, the name of its gene product, and a description... plasmid features such as antibiotic resistance genes, selectable markers, promoters, origins, tags, and... Overview of SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid...powered by GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software...the features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies ...scientists) for suggesting these changes, and the SnapGene and Addgene software development teams for working tirelessly...example below. Our old map is on the left while the SnapGene powered map is on the right (click here to see...

  10. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube! In this series...

  11. The Addgene Antibody Data Hub

    Type
    Blog Post
    ... page of the Addgene Data Hub.   Antibody data reports can be searched and sorted by gene/target, name... on addgene.org Addgene's Antibody Collection Addgene's AAV Data Hub More resources on the Addgene blog... request for your experiment!  Addgene’s Antibody Data Hub Addgene’s Antibody Data Hub can help with ...open-access Data Hub, researchers interested in the Addgene antibody collection can look at user-deposited ...experimental conditions in which the data was generated, you can scroll down the page to the materials...each data submission we receive is checked by Addgene's curation team for accuracy and completeness. The... citation. If you’re considering requesting an Addgene antibody, the Data Hub can help you decide if an...

  12. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each and every one...This year marks Addgene's 20th anniversary! We've been celebrating throughout the year, and on Sunday... to have many of our remote workers travel to Addgene's headquarters in Watertown, Massachusetts, for ...enjoying the party. There are so many teams that make Addgene functional, handling everything from facilities...  Throughout the party, we shared memories of Addgene's past. Framed photos and slideshows shared some...including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable collection of memorabilia...our co-ops to our directors, we can't celebrate Addgene without recognizing our incredible depositors and...

  13. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple ... the lab as well. For a more in-depth look at Addgene’s current lab-oriented environmental efforts check... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

  14. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...social media. #AddgeneCRISPRweek on Twitter From May 13 (today!) to May 17, the Addgene Twitter feed will...CRISPR overview and history. Tuesday will focus on generating knockouts using CRISPR and Wednesday will highlight...CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not...

  15. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...Addgene is excited to announce the launch of our partnership with the Institute for Protein Innovation... of integrin antibodies, will be available on Addgene’s website this fall. IPI antibodies will come ... antibody sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access...developed to support use of their antibody tools. Addgene also offers a growing collection of educational...

  16. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    ...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

  17. How Addgene Creates Content

    Type
    Blog Post
    ...everything else we do! The goal of Addgene’s educational content Addgene truly believes that sharing speeds...you ever wondered how we choose topics for the Addgene blog? Or why we make certain videos? In this post...You probably won’t be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs...topics. What better space to do that than in the Addgene blog?  Fig. 1: One of our favorite content...content collections is our Help Center, help.addgene.org, where you can easily search articles answering ...sure things are clear, accurate, and in line with Addgene style.        Fig. 2: Reference image (...already comes from you and the questions you ask Addgene and your research community! We work to provide...

  18. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... also modify other properties such as endogenous gene expression, which can lead to changes in normal ...neuronal firing. What is chemogenetics? Chemogenetics relies on genetically introducing engineered receptors... overview of the chemogenetics system. Why use chemogenetics Because chemogenetic receptors are activated...neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide Find viral preps...equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics are instead that it is...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose...most common target for chemogenetic manipulation are neurons where chemogenetic receptors can be used ...

  19. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These... We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate ...vital during this period of constrained funding. Addgene is uniquely positioned to help research institutions...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...scientific progress depends on our collective response. Addgene stands ready to collaborate with partners across...work and what support would be most valuable from Addgene during this time. Your input will directly shape...scientific community. Let us know by emailing help@addgene.org. We look forward to hearing from you!...

  20. Addgene: The First Twenty Years

    Type
    Blog Post
    ...allow people to add a gene to a repository others could request from — Addgene!  Addgene was established as...information across the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, ... which continue to grow today. “I generally refer people to Addgene for the basics of molecular biology...celebrating Addgene's 10 year anniversary.   Expanding our work In March 2015, Addgene reached the...a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the Society...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing service to biology...
Showing: 401 - 420 of 1145 results