We narrowed to 933 results for: KIN

...competent lentiviruses (RCL) especially if you are working on HIV positive human cell from the clinic. The...extra care has to be taken especially if you are working with proto-oncogenes or with human samples that... is now used as it shows rapid gene expression kinetics as compared to the Tet-off system. One drawback...one with either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express...transgene resulting in low inducibility and potentially making it difficult to interpret your results. This can...

...a startup and back to academia. Chapter 1: How working at a startup prepared Tom for an academic career...more complicated biological devices. Chapter 6: - Making research go faster through Open Access. Chapter...investigate synthetic biology at Boston University, working in one of the founding groups of the field under... Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with Michael...

...when did you start thinking about them? 18:30 - 19:54 : How to think about picking a post-doc project ... you leverage your community, think hard about picking a good mentor, and begin considering career options...Mentoring" We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them...

... including that it’s never too early to start networking! Learning about careers at Addgene Afterwards...popular, with the students excited to see a real working lab full of busy Addgenies preparing plasmid samples...impressed our guests, as did how much work is put into making sure scientists all over the world know that we...Advice from Our Bloggers Learn How to Improve Your Networking Skills  ...

...providing quality control and customer support, making plasmids easy for you to find and request in our... Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual plasmids ...Stabs are organized by shipment and moved to our packing area.Using the barcode system, the correct plasmids...necessitate an office move. We've got ~50 Addgenies working to ship plasmids, analyze QC sequence, answer scientists...

...Brownstein, an Addgene board member. When I was working in Human Resources at Harvard Medical School, Joanna...Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join their newly... her forever home. I feel blessed that I’m not working so that I’m available to spend time caring for ...for my mother. For fun, I enjoy pilates, jewelry making, reading, sailing, and gardening. Learn more about...

...expression cassettes (Shaw et al., 2023). Those looking to express high levels of their gene of interest...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest...requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless...mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was designed to work in....2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports...

... don’t want to spend months doing surgeries and making fiber optic cables only to hook up your lasers ...alter the light spectrum for activation and the kinetics of opsin activity/inactivity. Dr. Deisseroth has...(not expressing the opsin) are also available. Picking your opsin  You must consider whether you wish ...neuronal firing, the cell-type specificity, the kinetics of the opsin variant, and other particularities...the promoter of the cre recombinase while still taking advantage of the robust expression via the promoter...

...Tools for Making Scientific Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific...we're gearing up to start a new decade, we’re also looking back at the last year of scientific discovery. ...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest...

...Francisco International Airport listening to the Lion King soundtrack and writing this post, it is my pleasure...what new post contributed the most to this record breaking month and to find other posts that deserve a second...Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual Plasmid... Research Community Grad School Advice Part 1: Picking a Lab and a Project That’s it for now. As I indicated...

...at a lindy hop swing dance event,” Zheng says (networking can happen anywhere!). The pair quickly teamed... in November 2017. #Phage researchers! I am working with a team to get Burkholderia cepacia phages ...must recognize a specific part on the bacterium making each phage is very specific. Some infect only one...about scientific sharing Find blog posts about networking Additional resources on Addgene.org Find molecular...

...experiment. (And you’re certainly not alone – we’re all looking for high-quality antibodies and reagents that work...and sorting. Reference images can be viewed by clicking on the links in the "Agree" and "Disagree" columns...associated metadata adapted from the Affinity Reagent Working Group of the Human Biomolecular Atlas Program (...community when designing the IBEX Knowledge-Base, seeking to create an iterative process that is constantly...

...excited to finally share with you what we’ve been working on behind the scenes here at Addgene.  Addgene's...messaging, as well as simplifying the page, and making it all look clean and easy to use.  Easily search...global navigation and menus over the past year, making it much easier to get around our site.  Community...smile, but occasionally you might notice Blugene looking sad, especially if an experiment doesn’t go as ...

... search. This is especially true of scientists seeking a first job after the academic bench. Utilizing... the job posting instructs even if you have a networking connection that will be helping (see below). ...strengths in your resume and don't be shy about asking for help. The worst thing that can happen is someone... scientist networking...

...,” said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate the...For more information on choosing a backbone and making fusions, see this blog post from Joachim Goedhart...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many... many more! Baird has a helpful tip for anyone looking to peruse the collection without getting overwhelmed...

...faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow...set of tools therefore depends on what you are looking to study. Figure 1: A schematic showing the...receptors in the brain. One way to address this is by working with organisms with light accessible brains, such...

...your plasmids are distributed. Next month, we are making two changes to the policy that will make the process...the plasmids online for distribution instead of risking leaving them on hold indefinitely. Of course, the...citation and release the hold. Increasing impact Making the plasmids available as soon as your paper is... paper comes out later. Or, if a project isn’t working out as planned, but you still hope somebody will...

...paper they’re reading has been linked in a ReFigure making it easy for others to discover your insights on...researcher Christa Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure...PubMed Central, eLife, PLOS and Figshare but we are working on expanding our reach. We can’t save you the hard...career researcher and cofounded ReFigure with Joe Akin. She envisions a world where the impact of teaching...

...and you are smart, you can be a good manager. The kind of smarts and the type of skills that it takes to... from being included in organizational decision-making. It is imperative that employees are thanked for...manager keeps these factors in mind at all times when making decisions, assignments and polices. Sometimes the...priorities for advancement in science Have their own working schedules and may not necessarily be comfortable...

...persuing milestones and working hard. Being patient, being selective, and talking to peers and mentors will...with the current technology to identify a problem, asking what approach will I take and what can I learn,... Dr. George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists...