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We narrowed to 1,018 results for: LEA

Showing: 401 - 420 of 1018 results

  1. Running for Rare Disease, Running for FOP, Running for AJ

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    Blog Post
    ...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue to...being with the company for about six months, I learned there was a Genzyme-based running team called Running...Second, Addgene will be hosting the AJ’s Boston League team (Contributing many new heroes to the team ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease ...

  2. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post
    ...engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the...reagents so freely with the academic community has clearly made a huge impact on this field. Advances in ...described a chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target... service questions for nearly 7 years, and my colleagues and I have an internal motto for scientists who..., and I found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book...

  3. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...ever like to write about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...increasing the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described... rapid quantification of base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk...show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying...

  4. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    Blog Post
    ...Foundation, ABRC’s outreach program kicked off with the release of 20 education kits designed for use in K-12 and...ABRC database and ordering platform soon to be released. For individuals interested in ordering from ABRC...has many benefits. Engaging students in active learning through hands-on experiments allows them to develop... out opportunities to engage people in science learning. Whether visiting classrooms, hosting field trips....     Additional Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic Biology into ...

  5. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post
    ...Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies... relatively low efficiency of biotinylation, at least in vitro. Our solution was to exploit the best of...proteins that lacked TEV. Although we were unable to cleave TEV within the protein we tested, it may be possible...plasmid in NEB T7 SHuffle bacteria (C3029J). We are clearly NEB “fanboys” as the bacteria significantly enhance...

  6. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    Blog Post
    ...Figure 3: When a bison wanders into the campsite, leave your samples behind! Paul and Anastasia (Potanina...hyperthermostable Cas9 This year it was wonderful to learn that one project led by postdoc Dr. Stephanie Schmidt... IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein...for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps...useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR ...

  7. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post
    ...structure-guided direct evolution, Nathan Shaner and colleagues from The Scintillon Institute, Allele Biotechnology...Clavularia sp. protein CFP484. Hui-wang Ai and colleagues from the University of Alberta and University... in a 2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1 can be used...would like to share with the scientific community, please deposit the plasmids at Addgene! References Ai... to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection...

  8. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post
    ...Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral...incorporate into their own labs. This week we are pleased to announce the submission of the results of our... cells (Vandenberghe, 2010). Rather than being released into the cell culture supernatant, the viral particles..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...

  9. Custom CRISPR Screens & the Green Listed Software

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    Blog Post
    ...contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for Molecular...design gRNAs for all the purposes discussed above. Please see videos and text on the website for more detailed...have been “green listed” by several algorithms. Please read the “Detailed Information” text linked to ...and libraries using strategies related to other nucleases as well as inhibitory and activating approaches...PMC5292764. Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips...for a 1st CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries...

  10. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post
    ...attempted to study cancer from every possible angle to leave no stone unturned, but cancer has been producing...between tumors and the immune system We’ll begin by learning how the immune system naturally interacts with...Through interactions with the immune system, tumors “learn” how to escape it. In this process, cancer cells...tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment help create tolerance...enter the clinic, physicians and researchers are learning much about how treatment regimens can be improved...PMC3433802. Additional Resources on the Addgene Blog Learn About Our Cancer Collection Pages Plasmid Grant ...

  11. Antibodies 101: Introduction to Antibodies

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    Blog Post
    ...with a microbe, to trigger immune cascades that clear these infections. Anything that generates an antibody...immune system is referred to as an antigen. You can learn more about antibodies in our Antibodies 101 animation...isolate cells expressing this protein. Read on to learn more about antibodies and how to use them in the...produce is not necessarily what you are getting. Learn more about monoclonal antibodies here. Recombinant...simultaneously as well as compare between samples. Learn more about flow cytometry. Cell and tissue labeling...antibodies, the Developmental Studies Hybridoma Bank Learn more about nanobodies Watch our Antibodies 101 animation...

  12. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...excitation and emission spectra. Both spectra possess clear maxima where excitation and emission are the most... 500 nm and a global maximum at around 560 nm, leading to different levels of brightness, depending on...check out our blog post on color compensation to learn more about them. Alright, let's build your first...cell culture, the transduction process itself also leads to some cell death. As a result, there is a high...   Conclusion You made it! In this post, you learned about the principles behind fluorophores and their...this process creates visible colors. Next, you learned about how fluorophores can be classified into different...

  13. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post
    ...than 75% photobleaching after just 1 minute of exposure. MG-2I-dL5** experienced minor bleaching after longer...ablate cells in culture and in vivo.  Read on to learn more about this killer illumination technique! ...real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization and expressed from ...Sign Up to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on ...

  14. Pushing the Limits of DNA Assembly

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    Blog Post
    ...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...guide the joining reaction. BioBrick Assembly will leave scar regions at the site of fragment joining, but...still creating scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our Plasmids ...      Additional Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other...

  15. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ... of the mRNA, leaving only a red FP bound to the reporter. Because it contains a nuclear localization ...cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the segments of mRNA to which...occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated...Addgene. Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background...

  16. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post
    ...updates to iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here...give researchers the ability to monitor glutamate release from neurons and other brain cells in vivo. Glutamate...protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from the...differentiating between synaptic vs. extrasynaptic glutamate release. Building on the previous generation of the SF-...Resources Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors A Practical Approach...

  17. 25 Work From Home Tips for Scientists

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    Blog Post
    ...ride is a great activity for both exercise and clearing your mind. And because the outdoors is so huge...events. Download Addgene’s Science Career guide to learn more about career paths and things you can do to...Plan out meals and snacks ahead of time.  Find learning activities for kids. For example, Skype a Scientist...collected into figures for papers or presentations. Learn a new skill for analyzing and visualizing your data...introductions and literature reviews could make the months leading up to your defense less stressful. Think about...

  18. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post
    ...complex processes can’t be overstated, we can also learn a lot from Nature’s simpler solutions to transmitting..., and they can rupture E. coli spheroplasts to release encapsulated proteins. Video by ACS. For a more...appealing target for bioengineering, but it wasn’t clear whether they could 1) be decorated with proteins...Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi...

  19. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post
    ...libraries Learn about other members of the Addgene Advisory Board Additional Resources Learn a little...post-doc, Doench worked on using RNAi screens to learn more about cancer biology in Ed Harlow’s group at...and “with CRISPR, just the results are so much cleaner. The time frame from doing your primary screen ...Institute here Read other blog posts about CRISPR Learn more about the Broad Institute and the Platform ...

  20. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post
    ...illumination. This ability to reversibly bind and release a protein of interest in response to light allows...reaches the nucleus where gene expression changes lead to cell proliferation and differentiation. The lab...739201.  Additional resources on the Addgene blog Learn about using nanobodies to control protein activity...Read about RANbodies, reporter nanobody fusions Learn more about using the secondary nanobody toolbox ...Addgene.org Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics...
Showing: 401 - 420 of 1018 results