We narrowed to 785 results for: LIS;

...lentiCRISPR v2.(If you’ve peeked through the rest of the list at this point, you may have noticed that many other...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...

...Finally, as previously mentioned, enlist the help of your network to polish the final draft prior to submission...

...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...

...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...community to fulfill their mission. While they’ve shortlisted several protocols, including their phosphoserine...

...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which...on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy with AAV Figure...

...gene of poorly classified function and a well established cellular signaling gene, thus providing a starting...publications, we used the cancer plasmid collections listed above to demonstrate how gene expression profiling...

...plasmid carrying the required gene.  The table below lists some of the most commonly used selection markers...said element. This link provides a more extensive list of yeast auxotrophic markers and includes the associated...

...group of mentees to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical School... the internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings...

...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, Ramanathan...

...affinity reagents.    Types of Affinity Reagents  (Listed in no particular order.) Fab fragments are created...distinction of being the only affinity reagent on this list derived from nature. Single-domain antibodies are...

...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

...incorrect; take it instead as valuable feedback.  Listen. Think about the questions you’ve received, read...read the comments, seek feedback from others, and listen quietly as people discuss their experiences. If...

...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

...point, decide early on what to do in a namespace collision. It may even be worth it to have extra columns...solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence, insert, ...

...consider—we have loaded those we are aware of in our checklist for tissue and cell research. Using cells from...of this literature in our health and medicine checklist. A common worry for researchers wanting to consider...

...experiments The first thing you will need is to get the list of effective gRNA sequences targeting your genes...pool. (Adapted from Wong et al., 2016). With a list of gRNA targeting sequences, you can then readily...

... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt...(2014).   We talked with Voigt about this collision that’s taking place between CRISPR technology ...

... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse... transgenics. A few of the most common uses are listed below. Cre-dependent gene expression – placing...

...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...achieving each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls...