We narrowed to 928 results for: Mpl;

...the Gal4/UAS system. This incredibly useful, yet simple system allows you to systematically study gene ...downstream of a tissue-specific promoter sounds simpler, this modular system saves you from having to create... making a fly line in the next blog post). For example, the hedgehog gene can be overexpressed in several...under the control of UAS enhancer sequences. For example, if you discovered five new genes and wanted to...control purposes that there are dosage effects. For example, overexpression of two genes by one Gal4 driver...

...Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example Article Number...However, determining this exactly is difficult. For example, it is very plausible that science published in...research our adjustment controls for them.   So, for example, in 2010, 10 percent of the papers with Addgene...factors that could still be controlled for. As an example, plasmid-related areas of science could be growing...

...cellular level. The reason for this is cost and complexity – anyone who works in the lab knows how expensive...expensive tiny amounts of reagents are, how complicated (and again, expensive) the different machines are...students to use in the classroom because the students simply need to add water to express a variety of proteins... engaging for students. In our BioBits kit for example, we’ve designed FD-CF experiments that result in...Addgene.org Browse our protocol videos to learn simple lab techniques Check out our molecular biology ...

...was easy to find people who were interested. For example, our journal club discussion on Kevin Esvelt’s ... safety training prior to getting started. For example, Timothy Stiles joined BosLab last year with a ... space to perform early-stage experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab... getting the community to collect various soil samples, extract DNA, sequence it, and analyze the data... characterize the microbiomes of the different samples. The goal is to not only learn something about ...

...to target cells as a pooled library, which will amplify each TF to determine how its presence influences...processes. For help with library transformation and amplification, refer to the Zhang lab’s previously published...the specific dysregulation in the disease. For example, very few diseases manifest as a single mutation...expression dysregulation event. Diseases are often complex, with multiple layers of dysregulation from either...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library...

...make socially distanced work more creative. An example could be making lab meetings more interactive and...and brainstorm! Image from Joseph Mucira.   Implement new tools for creative interaction During Zoom...disappears from the video, and prepares a coffee, or simply moves away from the desk to switch off a few minutes...GA, O’Reilly CM, Lofton ME, Catalán N (2018) Ten simple rules for collaboratively writing a multi-authored...journal.pcbi.1006508 Vicens Q, Bourne PE (2007) Ten Simple Rules for a Successful Collaboration. PLoS Comput...

...particular reference sequence if necessary. The example plasmid on the right has a total size of 7.3kb,.... Avoid star activity. Some endonucleases (for example BamHI) are capable of cleaving sequences which ... The glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a ...you to interpret the bands that you get in your sample lanes. Choose your ladder based on the expected...approximate the DNA mass of comparably intense samples of similar size. Run the gel at 80-150V until you...

...for plasmid replication in mammalian cells, for example, and a toxin that kills bacteria may not have any...Selection methods, described below, are typically employed when creating stable cell lines and are not used...plasmid selection. Instead, reporter genes are often employed to easily monitor transfection efficiencies and...antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral EBV or SV40...HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes strand scissions 100-400 ug/mL...

...stable host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a...processes. The food processing industry, for instance, employs psychrophilic enzymes for milk processing, fruit...Additionally, the high activity of these enzymes implies that even a minute quantity can significantly reduce...

...lab or pivot to a managerial role. For those contemplating the leap into management, the journey can be...redefine the value I could bring to potential employers. Over time, I discovered that I not only enjoyed...motivations, strengths, and learning styles. For example, during my time as a lab scientist, I worked with...foster a cohesive, productive environment. For example, in my current role managing a cross-functional...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals...

...to physically set up in the lab, RNAi is often completely ineffective or results in low penetrant phenotypes...low-cost, cell-free detection assay that can be completed without special equipment. What’s more, detection... to be easily concentrated. Viral RNA is then amplified isothermally, and detected through a toehold switch...encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer....modifying the sequence of the toehold switch. For example, Green has also developed toehold switches to detect...

...just how much things have improved, check out the example below. Our old map is on the left while the SnapGene...on to learn more about the improvements we’ve implemented and how they’ll make it easier for you to find... next cloning experiment early on. Beyond these simple but powerful display improvements, when you click...or ORF to learn more information about it. For example, if you hover over the ampicillin resistance gene...software development teams for working tirelessly to implement these improvements. Let us know what you think...

...cancer precision medicine seems so simple! Take a patient’s tumor sample, use cutting edge genomic technologies... wild type, would be extremely helpful as a complementary approach. Also, in principle, these experiments...cancer phenotypes. These assays were designed to complement the one assay per gene approach that has been...genes. These should be used to create ever more complete “look-up-tables” of mutant function that could...

...endless ways and everyone finds their own. Here, I simply outline what works for me, and I hope that it is...experiments can be found in each folder. This can be a simple .txt document. Record the name, date, and location... work on multiple projects in a given day. For example, instead of naming a file “output” name the file...track of your exact changes. Alternatively, you can simply make a note in your methods section about the edit...result was, so try not to do this. These are the simple rules that I try to follow in my own research. ...

... Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation of endogenous... assemble a synthetic transcription activation complex consisting of multiple distinct effector domains...with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier by the Zhang lab for...sam.genome-engineering.org/. Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu H, Ran...transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, ...

...where one of the X chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic...translate those signals into cellular responses. For example, GPCRs and their downstream G protein partners ...with an emphasis on oligomerization, signalling complex assembly, and localization to the nucleus,” says...at McGill University.  These protein-receptor complexes consist of many parts that work together on the...gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of developmental delay, and...

...achieve, but are simple to pick up and work well the first time. But biology is complicated — so many different...reactive groups of Click Chemistry are almost completely absent from living systems. What are the possibilities...functionality to existing genes, while being accessible simply by dialing up the right nucleic acid sequence (...homology-directed repair efficiency by linking the repair template VirD2 HUH-tag For creating DNA-protein conjugates...

...problem.  Step Three: Decide on a strategy and implement it.  Step Four: Assess how effective your strategy...the cause. To test the most likely cause, you implement an experiment designed to fix the problem. Then...modifications, and ultimately decided on the changes to implement. As with any skill, there will always be room ...Know your strengths! Research experience presents ample opportunities to practice problem solving skills...problem solving skills can help convince a future employer that you are well equipped to work through challenges...

...based on size. Whether higher molecular weight complexes are detected will depend on the homodimer affinity...During the experiment, the solution is diluted, complicating the interpretation. Because of this, these methods...detecting homodimerization tendency. This assay employs a peptide derived from cytochrome p450, CytERM,...will be problem-free. In figure 1 we provide an example of the OSER assay with a number of fluorescent ...organisms that are engineered can be shared to simplify the comparison by others. Together, we can build...

... that actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting ...take up your insert. But exactly how does ccdB accomplish this? Let’s start with a brief history of the...system contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of...interest.   CcdB resistant E. coli strains complete the system But ccdB alone isn’t the only key to...protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3):735-45. PubMed...