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We narrowed to 72 results for: Mpl;

Showing: 1 - 20 of 72 results

  1. Antibody Guide

    Type
    Collection
    ...fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain fragment...application Collect and lyse samples to make proteins available. Tissue samples may need additional processing...proteins from a complex mix. Overview of application Process the tissue or cell culture samples to extract ...capture protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced...sequenced and amplified for analysis. Overview of application Process tissue or cell culture samples to extract...and lyse. Cross-link the samples with formaldehyde or UV light. Shear the samples using sonication to break...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...

  2. CRISPR Guide

    Type
    Collection
    ...genomic edits by copying from a DNA donor template . The donor template contains the desired sequence and is...linear dsDNA templates work better for large edits or insertions. When designing the donor template, incorporating...experiments and must be amplified before use (Figure 8C), following the amplification protocol specified by...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... change the genomic target of the Cas enzyme by simply changing the target sequence present in the gRNA...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...nuclease form a complex with each individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out ...Anti-CRISPR genes employ varied mechanisms to block CRISPR. Some prevent the CRISPR-Cas complex from binding...a protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy...out the DNA sequence complementary to the crRNA. In Type II CRISPR systems, a potential target sequence ...after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target...strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break. CRISPR systems...classes based on the structure of the effector complex that cleaves the genomic DNA. Type II CRISPR was...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...receptor GPR38, MTLR1 MPL myeloproliferative leukemia virus oncogene C-MPL, CD110, MPLV, TPOR MSTN myostatin...histocompatibility complex, class I, A FLJ26655, HLAA HLA-B major histocompatibility complex, class I, B AS...histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major histocompatibility complex, class II...histocompatibility complex, class II, DO beta DOB HLA-DPA1 major histocompatibility complex, class II, DP ...histocompatibility complex, class II, DP beta 1 DPB1, HLA-DP1B HLA-DQA1 major histocompatibility complex, class II...histocompatibility complex, class II, DQ alpha 2 HLA-DXA HLA-DQB1 major histocompatibility complex, class II, ...histocompatibility complex, class II, DR alpha HLA-DRA1 HLA-DRB1 major histocompatibility complex, class II, ...

  5. Plan Your Experiment

    Type
    Collection
    ... donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing...edits using CRISPR/Cas9 in mammalian cells as an example, but many of these principles also apply to using... process of interest. Do you want to: Generate complete and permanent loss of gene expression or function...Remember that HDR also requires donor DNA to serve as template for the repair. Once you settle on your gRNA, ...Editing : pegRNAs must serve as both a guide and a template. In general, edits must be downstream (3’) of ...edit site yield higher editing efficiencies. The template sequences can also have a large impact on editing...perfect world, your gRNA sequence would be an exact complement to your target sequence with no homologous sites...

  6. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... adenovirus or herpes simplex virus, to replicate in the host cell and complete the lytic cycle. To avoid...expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S...expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1...expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10...expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22...expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1... Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2...

  7. Zhang Lab CRISPR Page

    Type
    Collection
    ...SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous... : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with...vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral...SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (... In these mice the CRISPR-Cas9 system can be implemented by delivering Cre and sgRNA to a Cre-dependent...

  8. Serotype Testing AAV

    Type
    Collection
    ...for the 20 µL sized testing sample is denoted with a .T suffix (for example, AAV1.T). The catalog ID for...for the 100 µL sized sample does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing...different serotypes. In addition to the standard 100 µL sample size, the vectors listed below are also available...production page . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog...include virus associated DNA (vdna), which is a sample of the purified DNA from the original plasmid that...

  9. mTOR Pathway

    Type
    Collection
    ... kinase, is found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit...links to each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation... RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in brain S6K Also known...Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma...homolog Rictor RPTOR independent companion of MTOR, complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return...

  10. Viral Production

    Type
    Collection
    ... genomic DNA. This DNA is then used as a template to amplify a portion of the intended lentiviral insert...of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations are subjected to polyacrylamide...proteins as a fraction of total protein present in the sample is also determined and used to determine purity...shown relative to the total protein present in the sample. Sterility Viral vector preparations are added ...rigorous barcode matching system that follows the sample from the DNA tube all the way to the cleared viral...

  11. CRISPR Plasmids - Tagging

    Type
    Collection
    ...in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and...101 post for more information about HDR/repair template design. How to use CRISPR to tag your gene of ...endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to clone the...encodes both a guide RNA transcript and a repair template for an individual tagging event. The kit contains...Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous...

  12. Antibody Production

    Type
    Collection
    ...total protein content of the sample is determined. Only antibody samples with >90% purity are distributed...using a NanoDrop spectrophotometer. If needed, the sample is adjusted by diluting or concentrating to a final...Coomassie staining. For Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside...transfection. This residual plasmid is extracted and amplified with primers targeting the heavy chain and/or ...

  13. AAV Packaged on Request

    Type
    Collection
    ...including legal approval and an implementation letter DNA amplification Viral vector production Density...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such... Quality is our priority. During this time, we amplify DNA, produce the prep, and perform comprehensive...researchers with troubleshooting and results that complement published data, capturing the insights that often...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ... VP16 transcriptional activation domain). For simplicity, other promoter elements and transcription machinery... TetR with the activation domain of the herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This... downstream gene via its activation domain than simply removing a repressor. Tet-On has since become one...expression with flexibility for your experiments. For example, you could integrate a transactivator under a tissue-specific... as a dual vector design, but it is easier to implement and ensures that all transfected or transduced...induction, or amount of background expression. For example, only a relatively low level of doxycycline can...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    .... With its rapid development (embryogenesis is complete in ~3 days), short generation time (~3 months)...expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources can be found...visualize and track clones of cells over time. Examples of useful tools for studying cells that share ... Alex Schier Lab Other Tools of Interest A few examples of popular tools to use in zebrafish, ranging ...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... that has a different emission wavelength. For example, if the marker protein is fused to EGFP, you may...with their own unique function. The nucleus, for example, houses the cell’s genetic material. The mitochondria...Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection...

  17. CRISPR References and Information

    Type
    Collection
    ... you'll need Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond...intuitive interpretation of results produced by amplicon sequencing. The updated CRISPresso2 can analyze...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ...for NmCas9 and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49...CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Expression mPlum 590 649 4 4.5 100 min Monomer pQC mPlum IX - Mammalian Expression mPlum-N1 - Mammalian...Mammalian Expression mPlum-C1 - Mammalian Expression mPlum-pBAD - Bacterial Expression mRaspberry 598 625 13...? Visit our Empty Backbones page or search our complete collection and filter by vector type, popularity...

  19. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References The development of powerful...populations defined by multiple parameters. Figure 1: Examples of intersectional cell population definitions ...order to enable three-feature cell targeting. C) Example of viral targeting of cells expressing Cre AND ...producing a functional molecular tool (C,F). Implementation The following resources may be of interest ...interest for groups interesting in implementing intersectional experimental design. The Deisseroth Lab maintains...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...Fifrefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...-RLuc Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328...deep-learning approaches. Split Luciferase Complementation assays can report on protein-protein interactions... : Constructs for split firefly luciferase complementation assays in plants. Bioluminescence Resonance...
Showing: 1 - 20 of 72 results