We narrowed to 566 results for: PAC

1. Zinc Finger Consortium: Zinc Finger Arrays

   Type

   Collection

   ...specific vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression...

2. 2023 and 2024: Reflections and Looking Ahead

   Type

   Blog Post

   ...ll be celebrating with stories of innovation and impact made possible by the sharing of tools for research...

3. pSiM24: Simplifying Plant Genetic Engineering

   Type

   Blog Post

   ..., smaller binary vectors with a larger cloning capacity would be very useful: enter pSIM24! To build a...

4. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

   Type

   Blog Post

   ...speechless (the club of people who have been in space is pretty small). Therefore, I was delighted to ...

5. 9 Tips to Achieve Success in Academia

   Type

   Blog Post

   .... “There are many ways to have a large positive impact in society. We need much, much more science and...

6. Some Like it Hot: Thermostable GeoCas9

   Type

   Blog Post

   ... AA PAM Sequence NGG CRAA (R = A or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal...

7. Tips for Improving Your Next Manuscript

   Type

   Blog Post

   ... then the data produced isn’t likely to have an impact because it will be difficult or (more likely) impossible...

8. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

   Type

   Blog Post

   ...these small tokens of thanks for the incredible impact our depositors have had on Addgene and the scientific...

9. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   ...: Use a PAM-out configuration Optimize your spacing D10A: nick sites separated by 37-68 bp H840A: ...

10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type

    Blog Post

    ...Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated ...

11. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    ...Targeting Further restricted to illuminated region Impacts all cells expressing receptors Invasiveness Mice...

12. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type

    Blog Post

    ...your academic journey, remember to leave a little space for the unexpected — it might just be the key to...

13. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type

    Blog Post

    ...and offers a high degree of control over time and space. Zebrafish have become a popular model organism ...

14. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type

    Blog Post

    ...Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence, making it ready...

15. Antibodies 101: Fc Effector Functions

    Type

    Blog Post

    ...bind. We also know that changing the Fc domain can impact an antibody's binding affinity (Janda et al., 2016...

16. Cellosaurus: A Cell Line Information Database

    Type

    Blog Post

    ...production, SARS-CoV-2 research, or adenovirus packaging cell lines dataset collections.  Depending on ...

17. CASTing Off for New Shores in Human Genome Editing

    Type

    Blog Post

    ...power of CRISPR combined with the large sequence capacity of transposons open whole new worlds to explore...

18. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type

    Blog Post

    ... the academic community has clearly made a huge impact on this field. Advances in genome engineering ...

19. Management for Scientists: What Makes a Good Manager Anyway?

    Type

    Blog Post

    ...approached as peers rather than subordinates Need mental space and dislike intrusions (this actually varies, a ...

20. Choosing Your Perfect Empty Backbone

    Type

    Blog Post

    ...however, remember that you will need accessory packaging vectors to generate the virus. Bacterial vectors...