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We narrowed to 73 results for: PAC

Showing: 1 - 20 of 73 results

  1. AAV Packaged on Request

    Type
    Collection
    ... Viral Vector Packaging Service Packaged on Request AAV Packaged on Request Overview Details...Discover our AAV Packaged on Request service with all-in-one pricing for viral vector preps, which includes... in-stock capabilities. To meet that need, our Packaged on Request service lets scientists choose from...from over 4,000 transfer plasmids to be packaged in their choice of seven serotypes, resulting in over 28,000... 28,000 possible choices! By using AAV Packaged on Request, scientists can start experiments as soon as...purify, and titer viral vectors. Overview Our new Packaged on Request service is recommended for research-grade...serotypes and four volumes. Details Scale and Titer Packaged on Request can be ordered in four sizes. For all...

  2. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of... of Service Addgene's Packaged on Request Service is subject to Addgene’s Terms of Purchase and the applicable...LULLs). Titer For AAV preps produced through our Packaged on Request service, Addgene guarantees a titer...original order. Turnaround Time Production of a Packaged on Request material is generally ready for shipment...

  3. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ... Depositor Collections IGVF Consortium Impact of Genomic Variation on Function (IGVF) Consortium... a new window) (Link opens in a new window) The Impact of Genomic Variation on Function (IGVF) Consortium...variation affects genome function, which in turn impacts phenotype. The National Human Genome Research Institute...These joint efforts will produce a catalog of the impact of genomic variants on genome function and phenotypes...systematically perturbs the genome to assess the impact of genomic variation on genome function and phenotype...

  4. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...ID Name 13421 pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424 KJBAC1 strain 21869 pKJ1267 (aka pAC-alphaGal4) 21870... consists of 5 individually packaged plasmids and 3 individually packaged strains, which are shipped as...

  5. Retrovirus Plasmids

    Type
    Collection
    ...version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart 20672 MSCV-IRES-GFP MSCV For transgene...flanked by LTRs Packaging plasmid — contains packaging genes Envelope plasmid — contains packaging gene env ...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional Resources...highlights popular gamma-retroviral transfer, packaging, and envelope plasmids. Read our Gamma-retroviral...Sabatini Return to top Popular Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC ...8454 pCMV-VSV-G VSV-G envelope protein. Use with packaging plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...imperfect miRNA sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions...Optical reporter of presynaptic ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression of Renilla... 83280 pscAAV-CAG-RLuc Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...-Puro 72255 Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-... following protocol detailing the process for packaging these transfer vectors and generating new viral... WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene...

  8. CRISPR References and Information

    Type
    Collection
    ...expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92 , pAC93 , pAC94... Fly: gRNA cloning pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF,...pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148 , pAC149 ; Gateway destination...2014) (Link opens in a new window) . protoSpaceJAM protoSpaceJAM is an all-around platform for CRISPR ...destination: pAC90 PDF, 1.1 MB Katic Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274...PDF, 129 KB Marraffini Bacteria: pCRISPR new spacer cloning pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian...PDF, 255 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP...

  9. Bacterial Expression Systems

    Type
    Collection
    ... (vitamin B12) Escherichia coli Remy Loris 47663 pAC-EsaR-I70V Plac 3OC6HSL (Quorum sensing molecule) ... direct recombinant proteins to the periplasmic space between the inner and outer membranes. Targeting... 113634 pSCrhaB2 PrhaB Rhamnose Burkholderia cenocepacia Miguel Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI yes, cut S. pyogenes...pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Ebert pL-CRISPR.EFS.PAC 57828...pyogenes Duchek pAC155-pCR8-sgExpression 49045 Mammalian BbsI none S. pyogenes Jaenisch pAC153-dual-dCas9VP96...vector 51132 Mammalian BsaI none S. pyogenes Huang pAC154-dual-dCas9VP160-sgExpression 48240 Mammalian BbsI...pyogenes tagRFP Ebert pFGC-pcoCas9 52256 Plant AscI, PacI, SbfI yes, cut S. pyogenes Sheen pT7-gRNA 46759 ...pDCC5 59984 Fly BbsI yes, nick S. pyogenes Duchek pAC5-dual-dCas9VP48-sgTetO 48237 Mammalian BbsI yes, ...Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC 57829 Mammalian/Lentiviral BsmBI yes, cut S...

  11. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ..., viral packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids...encoded in the packaging plasmid will determine the AAV vector serotype. Some packaging plasmids also ...the helper plasmid. Packaging Plasmids Available with Addgene's Viral Vector Packaging Service This table...table lists the packaging plasmids (Rep/Cap plasmids) used by Addgene's Viral Vector Packaging Service to ...AAV packaging plasmid, expressing Rep2 and Cap1 James M. Wilson 232199 pAAV2/2 AAV2 AAV packaging plasmid...AAV packaging plasmid, expressing Rep2 and Cap7 James M. Wilson 112864 pAAV2/8 AAV8 AAV packaging plasmid...Gradinaru All Available AAV Packaging Plasmids This table lists all packaging plasmids (Rep/Cap plasmids...

  12. Genetic Code Expansion

    Type
    Collection
    ...Huiwang Ai 73544 pEvol-pAcFRS.2.t1 pAcFRS.2.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren... Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren...141173 pAcBac1-3nitroY-A7-RS A7 3nY tRNA synthetase M. barkeri Mammalian Ryan Mehl 141174 pAcBac1-haloTyrRS...acid Bacerial, Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli... amino acids Mammalian TAG Peter Schultz 50832 pAcBac1.tR4-MbPyl PylRS M. barkeri variety of unnatural...barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84...barkeri acridone Bacterial TAG Ryan Mehl 174101 pAcBac1.tR4-AcdRS82 Acridone 82 tRNA synthetase M. barkeri...

  13. Viral Vector Guides and Plasmids

    Type
    Collection
    ... transgene, packaging capacity, and size. Vector Type Expression Genome Packaging Capacity Virus Size ...host genome. AAVs are limited by their small packaging capacity. Gamma-retroviral Vector Guide | Gamma-retroviral...Adenoviral Plasmids Adenoviral vectors have high packaging capacity, high transduction efficiency, and high but...Interested in Addgene's viral services? See Addgene's Packaged on Request service for made-to-order AAV vectors...engineered such that they can be delivered to packaging cell lines and use their cellular machinery to...the viral genome to assemble the viral capsids (packaging and envelope plasmids). When transfected into ...Resource Guide . Resources Addgene’s Viral Vector Packaging Service Addgene's Viral Protocols Addgene Blog...

  14. CRISPR Guide

    Type
    Collection
    ...referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before...for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic target to be modified....target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)... an active, DNA-binding configuration, with the spacer region of the gRNA left free to interact with the...Cas9 will only cleave a given locus if the gRNA spacer sequence shares sufficient homology with the target...throughout the genome, called off-targets, that can impact your experiment. There are many online tools available...Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading and discrimination...

  15. Lentivirus Plasmids

    Type
    Collection
    ...shRNA of interest flanked by LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...Lentiviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset... third-generation Envelope plasmid — contains packaging gene env This collection highlights popular second...second- and third-generation lentiviral packaging, envelope, and transfer plasmids available at Addgene.... Feng Zhang Return to top Popular Lentiviral Packaging Plasmids ID Plasmid Generation Description PI ...generation transfer plasmids along with additional packaging components. Didier Trono 12260 psPAX2 2nd Contains...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...contains many unique protospacer sequences that have homology to foreign DNA. Protospacers are separated by...Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially...contain a species-specific sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds...sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly after where the... development has occurred at a truly astonishing pace, with work directed towards increasing on-target...fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very low off-target activity due to rationally...SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have distinct PAM ...

  17. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...COVID-19 collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping...leukemia virus, and vesicular stomatitis virus G) are packaged as replication-defective virus particles with ...SARS-CoV-2 and other coronaviruses using appropriate packaging plasmids. Use the search bar to find a particular...-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that...

  18. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ... Viral Vector Packaging Service AAV University of Florida Serotype Testing...Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype Testing... directly to the brain parenchyma or subretinal space Serotypes The following serotypes were developed...acknowledge Shannon Boye and cite Boye, et al. 2016. Impact of Heparan Sulfate Binding on Transduction of Retina...32304666 Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  19. AAV Viral Preps

    Type
    Collection
    ... (AAV) available from Addgene's viral vectory packaging service. Browse constructs currently available... Viral Vector Packaging Service AAV Browse In-Stock AAV Adeno-associated virus (AAV...can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose a transfer ...transfer plasmid from our repository and request packaging in one of seven serotypes (AAV1, AAV2, AAV5, AAV6,... For more information, visit our Viral Vector Packaging Service overview. Function Chemogenetics DREADDs...

  20. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... Viral Vector Packaging Service Addgene offers flexible packaging options so you can easily...AAV Browse Lentivirus Packaged on Request Choose from 30,000+ options, packaged just for you in 6-8 weeks...weeks. Learn about Packaged on Request Production and Quality Control Production Our production process...
Showing: 1 - 20 of 73 results