We narrowed to 622 results for: SET

...bidirectional USA-Europe professional collaborations, setting up bases and contacts with Spanish laboratories...Universities in USA and Europe (Harvard University, Massachusetts Institute of Technology, Boston University, ...

...microscope stage) Reagents None needed Procedure Set up your microscope by removing any protective cover...

...antibody is used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the...another great option to enhance resolution. In this setup, gels have an increasing range of acrylamide content...

...RNA expression of your gene of interest across a set of human tissues.  From this data, you see that there...and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want to use an antibody...

...each individual target is stained correctly Image a set of samples where you label only one target in each...

...responsibility, repeated reminders of blunders, setting target up to fail, shifting goal posts without ...has no idea who is dating whom Expanding your skillset Scientists are lifelong learners. I believe we...

...use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired immune system in bacteria...Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing...

...initiates different immune cascades. In the research setting, antibodies of different isotypes can be used together...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

...large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467..._style":true,"button_font":{"color":"#FFFFFF","font_set":"DEFAULT","size_unit":"px","font":"helvetica"...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using genome-wide...

...different-sized multimers, you may even see different sets of bands on the gel corresponding to each type of...

... discovery of RNA guided transposons in bacteria sets the stage for combining the super powers of CRISPR... transposons encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to...

... done things that I never even imagined before I set foot in the lab. They’re also more than eager to ...

...its expression, and is flanked (“floxed”) by two sets of incompatible recognition sites (e.g., loxP and...

...is orthogonal to their scientific research skill set. You'll need to motivate your trainees, communicate...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...

...solidified. Pro-Tip If you are in a hurry, the gel will set more quickly if you place the gel tray at 4 °C earlier...increases the density of your DNA sample causing it settle to the bottom of the gel well, instead of diffusing...

...tube for 5 min at room temperature on the highest setting. Note: You should see clearly separated layers:...

... Mello labs also identified several experimental set ups that could improve CRISPR efficiency in C. elegans...introduced. Cre-mediated excision of the selective cassette minimizes the sequence added, leaving a 34 bp ..., however the development of the self-excising cassette (Dickinson et al., 2015) made this step as simple...Engineering with a Self-Excising Drug Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-CRISPR...

... easier to automate and use in high-throughput settings, but when it comes to low-throughput applications...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...reactions with relatively little work after the initial setup. (Addgene actually uses some of these — if you’re...

...microscopist at your institution when heading into a new set of experiments. Many thanks to our guest blogger...number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and off. This can provide...