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TypeBlog Post...classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be...isn’t always the winner!). Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted...
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Viral Vectors 101: Viral Vector Elements
TypeBlog Post...For safety and production reasons, multiple plasmids with many components are used to package lab grown...will discuss them together. Gene expression The reason for most viral experiments is to deliver a genetic... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...mesophilic systems are the psychrophilic enzymes. The reason behind this isn’t really hard to guess. Think about...for all the Indian iGEM teams to interact with personnel from the industry and brainstorm with each other... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...started working in a new lab and I was the only person in the lab. When I saw a request for an Addgene...scientific conference, whether you are attending in person or monitoring that conference via social media.... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...fluorescent protein can be identified by a head-to-head comparison of several fluorescent proteins of the same spectral.... In figure 1 we provide an example of such a comparison between cyan fluorescent proteins using widefield... -
Addgene: The First Twenty Years
TypeBlog Post...molecular biology and cloning, since they have reasonable introductions to lots of molecular biology,” ... Rachel Leeson... -
Antibodies 101: Fab Fragments
TypeBlog Post...world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa. Figure 2: Comparison between a full IgG antibody and common antigen... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...not at all obvious, intuitive, or logical to a layperson. When you’re communicating ideas that you think... Rachel Leeson... -
Antibodies 101: Affinity Reagents
TypeBlog Post...entirely new affinity reagent. Fig. 1: A comparison of an antibody (left) to several alternative ... Rachel Leeson... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...talk. That moment taught me an incredibly valuable lesson: Your presentation will be better if you take time... Rachel Leeson... -
The time and cost required to make a plasmid
TypeBlog Post...written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make...if budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...interest and its localization in the membrane. The lesson we learned from these studies is that the tag protein...Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (1988) Purification of ... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post...leads with excellence. They are one of the main reasons I have so few worries and can often focus on the... reflect this diversity, remarkably so for a 90 person company. We were and remain ahead of most companies... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...the first author of this article, Dr. Douglas Richardson, recently contributed a relevant post to the ...Addgene blog. Find the SRpHi Biosensors here. Richardson et al. Nat Communications. 2017. PubMed PMID:... -
Developing Lab Management Software for Biology
TypeBlog Post...cracks. In this post we’ll highlight some of the lessons we’ve learned over the years with the hope that...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're working... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...cloning methods. Exonuclease-based methods like Gibson assembly require 20-40 bp of homology at the ends...using an alternative method like Gateway cloning or Gibson assembly. Another important consideration is the... -
Working with Nuclear Receptors
TypeBlog Post...making them available to all researchers for a reasonable fee. We at NURSA recognize the importance of ...at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...and Casɸ-3. This system is notable for several reasons. Casɸ is only ~70kDa, with a molecular weight roughly...the library here New from the viral service By Jason Nasse We regularly add new viral aliquots from our... -
Hot Plasmids - October 2020
TypeBlog Post...and inhibitory synaptic proteins with FingRs By Jason Nasse Since the pioneering days of Golgi and Cajal...library from Addgene New from the viral service By Jason Nasse We regularly add new viral aliquots from our...