We narrowed to 729 results for: STO

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether at a scientific...

2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...reaffirming.” Now, she’s an Associate Scientist at Bristol Myers Squibb and also writes for the Addgene blog...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal...

3. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   ...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...be the focus of your poster - the data and your story. If you choose a single-color background but want...

4. Engaging with science and society at pgEd

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   ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical... talk about science in a language that can be understood by those who are not experts in particular scientific...

5. Hot Plasmids - October 2020

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   ...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for  observation in live cells....

6. Plasmids 101: Terminators and PolyA signals

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   ... we'll provide an overview on how transcription stops, or termination. Read on to learn more! Download...basic processes for eukaryotic termination are understood and it has been noted that each eukaryotic RNA...

7. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...genetics and anatomy, which are not yet fully understood. We attempt to deconstruct and understand this... into it! If you’re in the Victoria area, please stop by. And if not, I suspect you can find a makerspace...

8. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   ... 5) Listen to your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor...Look at the problem from their viewpoint, and brainstorm about solutions that will meet both of your needs...

9. Hot Plasmids: Summer 2025

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   ... skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have shown that AAV11...purification, and titration. Can't wait? Browse our In-Stock AAV inventory or use our Packaged on Request service...

10. Degrading DNA with Cascade-Cas3

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    ... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins...

11. Production of Virus in Insect Versus Mammalian Cells

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    ...particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the...

12. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...-3.13. To test xCas9 3.0-3.13, Hu et al. first restored catalytic residues D10 and H840. To ensure that...Plasmids for Mammalian Systems Learn about CRISPR History ...

13. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...see our plasmid collection from Sue Jaspersen (Stowers Institute) and Mark Prescott (Monash University...ImageJ plugins JACoP and Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software...

14. Polymerase Chain Reaction Overview and Applications

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    ...late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq DNA polymerase... low during the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction...

15. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...Each mRNA molecule in the library doesn’t encode a stop codon, which causes the ribosome to pause at the... Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann...

16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...levels. Chromoproteins in nature Compared to the history of fluorescent proteins in molecular biology research...research, chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored...

17. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...HDR. If the max CFD score is less than 0.03, it stops mutating. (This happens often because the HDR inserted...will check the CFD score. If it is below 0.03, it stops mutating. (Note: the most common synonym in the ...

18. The Scientific Conference Poster Session: Tips for Success

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    ...to tailor your presentation so that it can be understood by your audience. Use your poster as an aid: ... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...

19. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...restriction sites allowing you to easily create customized overhangs to assemble up to 6 different fragments...PCR process (the precise mechanism isn’t well understood). Finally, TOPO cloning is another good option...

20. 3D Printing Meets CRISPR Cas9

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    ...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...