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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 718 results for: STO

Showing: 401 - 420 of 718 results

  1. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...genetics and anatomy, which are not yet fully understood. We attempt to deconstruct and understand this... into it! If you’re in the Victoria area, please stop by. And if not, I suspect you can find a makerspace...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the...

  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...to tailor your presentation so that it can be understood by your audience. Use your poster as an aid: ... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...

  11. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq DNA polymerase... low during the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction...

  13. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

  14. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid, and...

  16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ... cassette into a given locus, just upstream of a stop codon. Briefly, the PITCh vector should be constructed...the GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro ...

  17. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...would be useful for their own research, and helps requestors use the plasmid in their own labs. We highly ...survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such as some...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...236.
 Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken...

  19. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...Addgene's new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our...
Showing: 401 - 420 of 718 results