We narrowed to 580 results for: SUB

1. Plasmids 101: Origin of Replication

   Type

   Blog Post

   ...results always pick a single colony and do not subculture directly from glycerol stocks, agar stabs, or...

2. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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   Blog Post

   ... effects of Addgene’s brief shut-down and the subsequent fluctuations in distribution. I don’t have...

3. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   ...species exist in the mix to serve as effective substrates for tagmentation-based library preparation procedures...

4. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   ... fluorescent protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids...

5. Viral Vectors 101: Transductions

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   Blog Post

   ...altered to make the virus specific for a type or subtype of cells. This allows an organism to be injected...

6. Antibodies 101: Chimeric Antibodies

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   Blog Post

   ...While in the majority of cases the differences are subtle, chimeric antibodies should be revalidated before...

7. Addgene Begins Distribution of Recombinant Antibodies

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   Blog Post

   ...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

8. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

9. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

   Type

   Blog Post

   ... (scFvs) and nanobodies. Addgene will produce a subset of this collection as ready-to-use-proteins to ...

10. New Viral Vectors - Winter 2025

    Type

    Blog Post

    ...Addgene's Data Hub can help, with over 100 user-submitted viral vector reports. And don't forget to use ...

11. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    ...to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for...

12. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    ...PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional regulator to modulate...

13. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post

    ...be ways to adapt the game to each a variety of subjects at many different education levels. If you modify...

14. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    ... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with...

15. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    ...mRNA to which they are bound. These regions are subsequently sequenced and aligned to the genome to determine...

16. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    ...which a relatively large number of samples are subjected to a DNA-barcoding step that uniquely labels DNA...

17. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    ...through a liquid to determine the concentration of substances in the liquid. Molecules absorb different wavelengths...

18. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type

    Blog Post

    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

19. Improving Transduction Efficiency with Exosome AAVs

    Type

    Blog Post

    ...with cell lysis to release AAV particles with subsequent purification steps. It’s also possible for AAV...

20. The MTA at Addgene

    Type

    Blog Post

    ...specified terms. This acts as the signature on any subsequent MTA for that plasmid facilitated by Addgene —...