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We narrowed to 618 results for: Set

Showing: 401 - 420 of 618 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using genome-wide...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...antibody is used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the...another great option to enhance resolution. In this setup, gels have an increasing range of acrylamide content...

  5. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired immune system in bacteria...Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing...

  6. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... discovery of RNA guided transposons in bacteria sets the stage for combining the super powers of CRISPR... transposons encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to...

  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...initiates different immune cascades. In the research setting, antibodies of different isotypes can be used together...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

  13. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...solidified. Pro-Tip If you are in a hurry, the gel will set more quickly if you place the gel tray at 4 °C earlier...increases the density of your DNA sample causing it settle to the bottom of the gel well, instead of diffusing...

  15. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... easier to automate and use in high-throughput settings, but when it comes to low-throughput applications...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...reactions with relatively little work after the initial setup. (Addgene actually uses some of these — if you’re...

  16. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ... Mello labs also identified several experimental set ups that could improve CRISPR efficiency in C. elegans...introduced. Cre-mediated excision of the selective cassette minimizes the sequence added, leaving a 34 bp ..., however the development of the self-excising cassette (Dickinson et al., 2015) made this step as simple...Engineering with a Self-Excising Drug Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-CRISPR...

  19. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...microscopist at your institution when heading into a new set of experiments. Many thanks to our guest blogger...number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and off. This can provide...
Showing: 401 - 420 of 618 results