We narrowed to 623 results for: Set

... this skill that you could strengthen. The right set of transferable skills, coupled with your technical...know you are bringing this asset to your job, you can become a valuable asset yourself.     Fig... point of view and can’t understand why they’re upset, conflict resolution is likely not one of your strengths...

...bidirectional USA-Europe professional collaborations, setting up bases and contacts with Spanish laboratories...Universities in USA and Europe (Harvard University, Massachusetts Institute of Technology, Boston University, ...

...microscope stage) Reagents None needed Procedure Set up your microscope by removing any protective cover...

...responsibility, repeated reminders of blunders, setting target up to fail, shifting goal posts without ...has no idea who is dating whom Expanding your skillset Scientists are lifelong learners. I believe we...

...each individual target is stained correctly Image a set of samples where you label only one target in each...

...RNA expression of your gene of interest across a set of human tissues.  From this data, you see that there...and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want to use an antibody...

...antibody is used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the...another great option to enhance resolution. In this setup, gels have an increasing range of acrylamide content...

...use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired immune system in bacteria...Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...

...is orthogonal to their scientific research skill set. You'll need to motivate your trainees, communicate...

...its expression, and is flanked (“floxed”) by two sets of incompatible recognition sites (e.g., loxP and...

...different-sized multimers, you may even see different sets of bands on the gel corresponding to each type of...

... discovery of RNA guided transposons in bacteria sets the stage for combining the super powers of CRISPR... transposons encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to...

... done things that I never even imagined before I set foot in the lab. They’re also more than eager to ...

...initiates different immune cascades. In the research setting, antibodies of different isotypes can be used together...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

...large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467..._style":true,"button_font":{"color":"#FFFFFF","font_set":"DEFAULT","size_unit":"px","font":"helvetica"...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using genome-wide...

...solidified. Pro-Tip If you are in a hurry, the gel will set more quickly if you place the gel tray at 4 °C earlier...increases the density of your DNA sample causing it settle to the bottom of the gel well, instead of diffusing...

...tube for 5 min at room temperature on the highest setting. Note: You should see clearly separated layers:...

...March for Science in Albany, NY, many tables were set up with various science demonstrations, and students...

...microscopist at your institution when heading into a new set of experiments. Many thanks to our guest blogger...number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and off. This can provide...