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  2. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...presence? Learn a key new job skill? Finish your thesis? Knowing your desired outcomes will help you assess...For example Dora Farkas, founder of Finish Your Thesis (who has also blogged for us) offers a free report...

  3. Working with Nuclear Receptors

    Type
    Blog Post
    ...as in important physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity...coregulators in HeLa cells was the first comprehensive analysis of the interactome of these key regulatory molecules...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...always helpful to remember that if a PI asks for assistance in the grant application process, she has faith...deem your proposal successful. Typical proposals consist of 3 to 5 aims, with secondary milestones to meet...

  7. Lab Automation at Addgene

    Type
    Blog Post
    ...fills all those vials? Well, it used to be a lab assistant, but with increased plasmid demand it was too ...fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    ...fluorescent timer emits blue light around the time of synthesis and, after a few hours, a conformational change...myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across these ...

  10. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...All destination vectors contain an ampicillin resistance cassette and an origin of replication, which ...pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...

  11. A Look at Addgene's QC Process

    Type
    Blog Post
    ...topics that may require additional attention during analysis. These include topics such as: locations in a ...on Addgene.org Educational Resources Sequence Analysis of a Plasmid Protocol ...

  13. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ... will be selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic... may be some low-level fluorescent protein or resistance marker expression following transfection, higher-level...

  14. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-... Hur JK, Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

  15. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination of the transcript...: PMC102582. Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion...

  16. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...will be erratic, opsin activation/inactivation inconsistent, and behavioral results impossible to interpret...sequentially increasing fineness helps to maintain a consistent stream of light. Test your implant by connecting...

  19. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...the cell pellet, I lyse the cells with this AAV lysis buffer and freeze/thaw the lystate four times in...-on time and 2 hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol...
Showing: 401 - 420 of 729 results