We narrowed to 731 results for: Sis;

... starting point for a new generation of ABEs), consists of 3 components:  a deaminase (TadA, originally...edited the fetal hemoglobin promoter and caused persistent expression of fetal hemoglobin to a greater level...

...trypsin digest, PPIs are identified via mass spec analysis. Biotinylated proteins are considered possible...remain stable (or to be stabilized) during cell lysis and lysate fractionation. Important considerations...

...fills all those vials? Well, it used to be a lab assistant, but with increased plasmid demand it was too ...fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand...

...Explorer, where you can also find the certificate of analysis provided with each cell line. The editing strategy...

...topics that may require additional attention during analysis. These include topics such as: locations in a ...on Addgene.org Educational Resources Sequence Analysis of a Plasmid Protocol ...

...All destination vectors contain an ampicillin resistance cassette and an origin of replication, which ...pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...

...community involved in sampling or bioinformatics analysis or upload your data to Zooniverse. The Natural..., Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a...

... will be selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic... may be some low-level fluorescent protein or resistance marker expression following transfection, higher-level...

...rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-... Hur JK, Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

...rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination of the transcript...: PMC102582. Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion...

...will be erratic, opsin activation/inactivation inconsistent, and behavioral results impossible to interpret...sequentially increasing fineness helps to maintain a consistent stream of light. Test your implant by connecting...

...transferable skills such as leadership skills can only assist you and make you more qualified for future career...blogger Juliet Moncaster! Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine...

...compartment was used to optimize D-glucaric acid biosynthesis. This MPyV system encapsulated a rate-limiting... a compact Cas9 ortholog from Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable...

... popular cloning methodologies, with a special emphasis on these five categories. Please note that many...backbone containing a topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated insert. When...

...to participate in genetic recombination during meiosis. Also, flies are not viable if they are homozygous...that males do not recombine chromosomes during meiosis. This can sometimes be a handy feature when setting...

...Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent protein ... Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically on...

... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...Dolan, A. E., Liao, M., & Ke, A. (2018). Structure basis for RNA-guided DNA degradation by Cascade and Cas3...

... easier, and more comprehensive thanks to the consistent development and improvement of CRISPR-based technologies...& Platt, R. J. (2023). Transcriptional linkage analysis with in vivo AAV-Perturb-seq. Nature, 622(7982...

...the virions out. We recommend using a Tris-based lysis buffer and sonication.  The obtained virions from...can then remove the density gradient material by dialysis/buffer exchange and further concentrate the virions...

...you can adjust compensation values later in your analysis, unlike voltages.  Figure 1A shows a single-color...always change your compensation values during the analysis portion of your experiment. So, as long as you...