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We narrowed to 740 results for: Sis;

Showing: 401 - 420 of 740 results

  1. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...antibiotic in the plate correspond to the antibiotic resistance marker present in your plasmid. You should also...is due to a PCR mistake or a mistake by the DNA analysis software. Now that you've read our plasmid troubleshooting...

  4. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with half the size...promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958...

  5. All in a Twist: dsRNA

    Type
    Blog Post
    ...centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to their abundance...considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that arise during transcription...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not...determine its genotype. However, this detailed analysis is not possible with IVF, as most of the cells...

  7. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...technical details of the problem you're solving, but consistency is key. Some namespace collisions you may encounter...treating these pieces of data in ways that are inconsistent with how they are being treated in the lab, ...

  9. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...presence? Learn a key new job skill? Finish your thesis? Knowing your desired outcomes will help you assess...For example Dora Farkas, founder of Finish Your Thesis (who has also blogged for us) offers a free report...

  10. Working with Nuclear Receptors

    Type
    Blog Post
    ...as in important physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity...coregulators in HeLa cells was the first comprehensive analysis of the interactome of these key regulatory molecules...

  11. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...always helpful to remember that if a PI asks for assistance in the grant application process, she has faith...deem your proposal successful. Typical proposals consist of 3 to 5 aims, with secondary milestones to meet...

  12. A Look at Addgene's QC Process

    Type
    Blog Post
    ...topics that may require additional attention during analysis. These include topics such as: locations in a ...on Addgene.org Educational Resources Sequence Analysis of a Plasmid Protocol ...

  13. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...All destination vectors contain an ampicillin resistance cassette and an origin of replication, which ...pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ...fluorescent timer emits blue light around the time of synthesis and, after a few hours, a conformational change...myeloid leukemia and saw that expression of H2B-FT consistently predicted the cell cycle speed across these ...

  17. Lab Automation at Addgene

    Type
    Blog Post
    ...fills all those vials? Well, it used to be a lab assistant, but with increased plasmid demand it was too ...fill 400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ... will be selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic... may be some low-level fluorescent protein or resistance marker expression following transfection, higher-level...
Showing: 401 - 420 of 740 results