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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: Sis;

Showing: 401 - 420 of 738 results

  1. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...systematically and logically is as useful for finishing your thesis as it is for helping your team reach an important...thinking, decision making, creativity, or data analysis. Conveniently, these are all skills you are already...

  2. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...technical details of the problem you're solving, but consistency is key. Some namespace collisions you may encounter...treating these pieces of data in ways that are inconsistent with how they are being treated in the lab, ...

  3. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not...determine its genotype. However, this detailed analysis is not possible with IVF, as most of the cells...

  4. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...

  5. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...antibiotic in the plate correspond to the antibiotic resistance marker present in your plasmid. You should also...is due to a PCR mistake or a mistake by the DNA analysis software. Now that you've read our plasmid troubleshooting...

  9. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...presence? Learn a key new job skill? Finish your thesis? Knowing your desired outcomes will help you assess...For example Dora Farkas, founder of Finish Your Thesis (who has also blogged for us) offers a free report...

  10. Working with Nuclear Receptors

    Type
    Blog Post
    ...as in important physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity...coregulators in HeLa cells was the first comprehensive analysis of the interactome of these key regulatory molecules...

  11. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...always helpful to remember that if a PI asks for assistance in the grant application process, she has faith...deem your proposal successful. Typical proposals consist of 3 to 5 aims, with secondary milestones to meet...

  12. A Look at Addgene's QC Process

    Type
    Blog Post
    ...topics that may require additional attention during analysis. These include topics such as: locations in a ...on Addgene.org Educational Resources Sequence Analysis of a Plasmid Protocol ...

  13. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...All destination vectors contain an ampicillin resistance cassette and an origin of replication, which ...pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...

  14. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ... will be selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic... may be some low-level fluorescent protein or resistance marker expression following transfection, higher-level...

  15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...rates, determined using sophisticated genome-wide analysis. At many of its computationally predicted off-... Hur JK, Been KW, Yoon SH, Kim JS. Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

  16. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination of the transcript...: PMC102582. Additional Resources A detailed analysis of yeast terminators of varying strength. A discussion...
Showing: 401 - 420 of 738 results