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  1. The time and cost required to make a plasmid

    Type
    Blog Post
    ...written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make...if budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’...

  2. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...leads with excellence. They are one of the main reasons I have so few worries and can often focus on the... reflect this diversity, remarkably so for a 90 person company. We were and remain ahead of most companies...

  3. Addgene: The First Twenty Years

    Type
    Blog Post
    ...molecular biology and cloning, since they have reasonable introductions to lots of molecular biology,” ... Rachel Leeson...

  4. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison between a full IgG antibody and common antigen...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative ...  Rachel Leeson...

  8. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...cracks. In this post we’ll highlight some of the lessons we’ve learned over the years with the hope that...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're working...

  9. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...cloning methods. Exonuclease-based methods like Gibson assembly require 20-40 bp of homology at the ends...using an alternative method like Gateway cloning or Gibson assembly. Another important consideration is the...

  10. Working with Nuclear Receptors

    Type
    Blog Post
    ...making them available to all researchers for a reasonable fee. We at NURSA recognize the importance of ...at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular...

  11. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...gene and cell-based therapies in the new era of personalized medicine (2017).   1st Gen AdVs are stripped...gene and cell-based therapies in the new era of personalized medicine." Genes & diseases 4.2 (2017): 43-63...

  12. Rabies and Neuronal Tracing

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    Blog Post
    ...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body’... 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic...

  13. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...20463753. PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ...and inhibitory synaptic proteins with FingRs By Jason Nasse Since the pioneering days of Golgi and Cajal...library from Addgene New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  16. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... Maya Peters Kostman in May 2020 and updated by Jason Nasse in July 2023.  References and Resources References...Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research...

  17. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibodies recognize some rat primary antibodies (Erickson 1993). It is always better to choose primary antibodies...Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). ...

  18. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...consists of highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged...degradation.      New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  19. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...and Casɸ-3. This system is notable for several reasons. Casɸ is only ~70kDa, with a molecular weight roughly...the library here New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  20. Tips for Technical Support Calls

    Type
    Blog Post
    ...! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet searches are done via...potentially faulty product. Please remember that the person at the other end of that email you’re writing is...
Showing: 401 - 420 of 721 results