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Addgene

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We narrowed to 14 results for: Son

Showing: 1 - 14 of 14 results

  1. Gibson Assembly Protocol

    Type
    Protocol
    ... Protocols Gibson Cloning Gibson Assembly Cloning You may also...to include in the Gibson reaction are different than with normal PCR primers (Gibson et al. , 2010). Number...new window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang...nmeth.1318 (Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). ...OpenWetWare: Gibson Assembly (Link opens in a new window) OpenWetWare: Janet Matsen’s guide to Gibson Assembly...Get started with Gibson Assembly Cloning! Protocols...Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute...

  2. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.... Protocols Personal Protective...Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs...

  3. AAV Production in HEK293 Cells

    Type
    Protocol
    ... of sonication to avoid overheating of the sample. Mix well between rounds of sonication. Sonicate until...container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T...completely. Combine all resuspended cell pellets and sonicate 5 x 1 sec pulses with at least 5 minutes on ice...

  5. Lentivirus ddPCR Titration

    Type
    Protocol
    ... two copies of RPP30 in diploid cells, thus the reason for multiplying by 2. $$V = 2*{copies\ RRE \over...Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...

  6. Weighing Reagents Protocol

    Type
    Protocol
    ...of how to weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the...

  7. Video Library

    Type
    Protocol
    ...key strategies of aseptic technique, including personal protective equipment, setting up a clean workspace...

  8. Centrifugation

    Type
    Protocol
    ...Centrifuge Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the...

  13. Western Blot

    Type
    Protocol
    ...most proteins but more stringent buffers and a sonication step may be required for hard to extract proteins...
Showing: 1 - 14 of 14 results