We narrowed to 844 results for: Tat

...have since been initiated and today thousands of mutations in genes affecting diverse biological processes—not...deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish using zinc-...depending on whether a gene is deactivated via mutation or morpholino. This observation unlocked novel... been tracked using microscopy and advanced computational approaches (Keller et al., 2008). This microscopy-based...

...  ICE uses Sanger sequencing data to produce quantitative, NGS-quality analysis of CRISPR editing - enabling...For a multiplex sample, ICE includes visual representations of all detected edit types in the sample. The...complete, a new screen appears with a graphical representation of the results and a list of the analyzed samples...in your edited population and their relative representation in the edited pool. The black vertical dotted...

..., allows us to display high-quality maps and annotations without us having to invest too much of our internal...partner, connect the relevant teams, coordinate implementation, and now must focus on managing the established...I personally felt that I had to overcome some hesitation to ‘approach strangers’ and learn how to communicate...maintaining useful notes that remind me about the current state of a partnership. Every collaboration and partnership...

...preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated pellets...i.e., genome-containing) AAV particles within representative vector preparations was quantified with electron...for viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids...

... constant variables that enable the correct interpretation of the effect of the independent variable you...controlled for in order to eliminate any alternative interpretation of the results. Typically, plasmid-based experiments...non-targeting control is critical to the correct interpretation of the results because it provides an important...etc, but outside constraints may impose some limitations and you should be cognizant of these when interpreting...

...sense OR antisense transcript depending on its orientation to your gene. T7lac High levels of gene expression...sense OR antisense transcript depending on its orientation to your gene. araBAD General expression Promoter... promoter with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus...sense OR antisense transcript depending on its orientation to your gene Although this list is a great...

...post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-CoV-2 has... fluorescence can be observed under either no excitation light, UV light or LED blue light.  Highlights...to our guest bloggers Shravanti Surest from Iowa State University. Shravanti Suresh is a PhD candidate ...CRISPR/Cas immune systems in the Sashital Lab, Iowa State University. She is actively interested in science...

...glutaraldehyde crosslinking: representative micrograph (C), particle orientation distribution and Fourier-...natively isolated complexes. To address these limitations, the Funabiki Lab developed a technique called...of LEAs may bias the distribution of particle orientations, this could be overcome with methods such as...

...recombination target sequences, denoted by triangles] facilitate tissue or cell type specificity. An on/off genetic...administered intravenously. To address these limitations, labs have developed techniques like CREATE (...broad):  enhanced coverage for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) Chan et al...AAV.CAP-B10 CNS: balanced coverage of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et... its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB (Fig. 4c, Jang et al....administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque ...

...hearing about researchers' favorite features and limitations of existing backbones, we're excited to share...5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts the identification...gene promoter using TurboCas, streptavidin immunoprecipitation (IP), and tandem mass tag (TMT) mass spectrometry...interneurons — those marked by parvalbumin (PV), somatostatin (SST), vasoactive intestinal peptide (VIP), ...

...which can be switched from a default OFF to an ON state, and how you might use these in your research. Today...which can be switched from a default ON to an OFF state, as well as repressible binary systems. In recent...domain VP16. cTA binds to promoters containing putative operator sequences (CuO) (linked in groups of ...QUAS promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase...

...first generate your desired mutation in mouse ES cells, select for the mutation and inject the ES cells into... models. For simple knockout alleles or point mutations, the 8-10 month process of conventional gene targeting...PAM; if not, you can add a silent PAM or guide mutation. Designing an Easi-CRISPR conditional allele The...

...nucleotide sequences with high specificity and quantitative accuracy and even include profiling single nucleotide...Electrochemiluminescent assay for detection of extremely rare mutations based on ligase reaction and bead enrichment."...direct identification of allelic composition of mutated DNA in a mixed population of excess wild‐type DNA...probe for the detection of low-abundance point mutations." Analytical chemistry 86.1 (2013): 900-906. PubMed...

...of the population as a whole, scientists gain statistical power in their observations. However, while flow...Techniques is Best for Me?  An overview of the instrumentation A flow cytometer combines three systems to ...fusions by binding to the protein in its native state.  Antibodies-based flow cytometry can use both direct...cytometry: retrospective, fundamentals and recent instrumentation. Cytotechnology 64:109–130 . https://doi.org...

...the product either via the Sodium Acetate/isopropanol precipitation method or using a silica spin column...column available from several vendors. The precipitation method has an unfortunate side effect of messing...

... V2.0 from it's predecessor, which had a point mutation in the puromycin resistance gene that made it ...less effective in certain cell lines. This point mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro...than creating double stand breaks and may better facilitate homology directed repair. You can find all of...

...is limited by definition, the kinome is a well-annotated collection of genes that can provide a useful ...useful for imaging or gene expression assays necessitating the screening of individual plasmids separately... gRNAs that score as hits in pooled screens, facilitating more rapid follow-up. We hope the biological...

...Also in June, the G8 Science Ministers issued a statement after a meeting in London on the global science...available to others got a nine percent bump in citations for their trouble, even when all kinds of other... as much about open access as it does about the state of peer review. 8)     That same issue of Science...

...eyestalk and transform them into a pulsating display imitating a moving caterpillar. The worm controls the snail...discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are lethal, associated with defective..."Tongue replacement in a marine fish (Lutjanus guttatus) by a parasitic isopod (Crustacea: Isopoda)." ...

...plasmid sharing during COVID-19 Realizing this limitation, we worked closely with the legal partners and...As infection rates were increasing in the United States, and many universities and Technology Transfer ...Proteins, and plasmids from several labs at Florida State University became openly available. Find plasmids...