... and open source software, hardware, wetware, technology development, etc). Second, the potential hire...job satisfaction (especially for scientists and technical people) is linked to engagement and access to ...you would like to head for a career in pharma or biotech, this is one case where the topic of your research...

...fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness is an important...brightness that does not fold well or cannot be detected in a microscopy setup has zero practical brightness...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...

...Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated... is one of the latest developments in CRISPR technologies.  It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...

...acids in the domain). Yan et al. from Arbor Biotechnologies found that RspCas13d accessory protein RspWYL1...functional in multiple species (read Arbor Biotechnologies' press release about their Cas13d deposit here...Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources on the Addgene Blog:...

... sharing, there will always be an interest in protecting intellectual property. Figuring out that balance...year and we have a very dominant presence in the biotech sector in both patent law and general life science...Young, LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance...

... creativity in employing a variety of cloning techniques and troubleshooting methodologies drew me to ...antibodies are commonly used in research laboratories to detect the different subclasses, but there are also secondary...use of subclass specific secondary antibodies to detect multiple targets with different mAbs in the same...

...biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques used, but...immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions...

...excitation by a laser, which is then read by a detector panel in the machine. Fluorophores are light sensitive...different signaling molecule, that all need to be detected in the same assay. This is one of the most complex...signal "spilling over" into another channel's detection range. Though you can compensate for this when...

... IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly... using monoclonal antibodies Read about other techniques that you can use antibodies for in our Intro ...

...features with gamma-retrovirus including genome architecture, host integration, and its association with ...Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713...An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390...

...gaps and add to them as techniques evolve. Blog posts get out of date as techniques and knowledge changes... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...

...heterotrimeric G protein complex that can be used to detect G protein activation via bioluminescence resonance...response in vivo (Paek et al., 2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation...are then identified with mass spec. Using this technique termed “GPCR-APEX” the Kruse lab was able track...

...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...PI labs and institutions. There are many more technical details that make our new search engine unique...

...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ... require access to next generation sequencing technology. These libraries are lentiviral in nature, ...

...to essentially any DNA sequence desired. This technology allows you to create a pair of designer proteins...on traditional homologous recombination. That technology had been used for decades to generate knock-out...That’s the revolutionary aspect: TALENs take a technology that was difficult for all but the most experienced...

... by the reliance on pH-based biosensors used to detect the maturation of neutral vesicles to more acidic...For further reading on fluorescent microscopy techniques, the first author of this article, Dr. Douglas...with classical gene overexpression and knockout techniques. Find the plasmids from Aranda-Díaz et al. Aranda-Díaz...

...Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural activity using a red fluorescent genetically...encoded voltage indicators (GEVIs) are proteins that detect and output the voltage changes in neural activity..., has increasingly been recognized for its biotechnological applications including conversion of CO2 into...

... an antibody that is directly conjugated to a detection substrate, such as a fluorophore, or a conjugated...do you know about the vendor and the vendor’s technical support? An antibody from a vendor that you use...frequently, with a responsive and knowledgeable technical support team, is a safer bet than one from a large...

...experiments that require multiple antibodies that each detect a different antigen. To pre-adsorb the antibodies...section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational...Overlooked benefits of using polyclonal antibodies. BioTechniques 65:127–136. https://doi.org/10.2144/btn-2018...

...it, his lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical... collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making ...all heading? Voigt: The top products out of biotechnology today are things like butanol - very simple ...