We narrowed to 750 results for: Uty

1. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   ... the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs were then verified...plasmids have been deposited with Addgene for distribution to the scientific community with the plasmid...

2. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   ...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ...

3. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   ... Rep68 to initiate replication and a terminal resolution site that serves as the target site for Rep proteins...structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with biorender....

4. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   ...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...

5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   ...genetic manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily...

6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   ...each genotype with vulnerabilities) to design a therapeutic strategy that works. But, those darn cancers ... using attributes of cancer variants such as evolutionary conservation, or predicted deleteriousness of...

7. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ... break site (Figure 2A). The single molecule resolution of digital PCR allows classification of individual...total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA from a few thousand...

8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   ...this same antibody to any protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small...thing to do to put these out there. It’s a simple solution. It would be silly not to share it as soon as ...

9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ...

10. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    ...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009...

11. Antibodies 101: Fab Fragments

    Type

    Blog Post

    ...improve localization accuracy when used in super-resolution microscopy by reducing the distance between the...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https...

12. Addgene: The First Twenty Years

    Type

    Blog Post

    ...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ...

13. To Each HIS Own

    Type

    Blog Post

    ...that of the protein of interest. A high imidazole elution buffer disrupts the interaction of the protein ... disrupt contaminant-metal ion binding without eluting the target protein and needs to be empirically ...

14. All in a Twist: dsRNA

    Type

    Blog Post

    ...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more...

15. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    ...Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-...

16. dTAG - You're it!

    Type

    Blog Post

    ..., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...

17. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    ...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...PMCID: PMC4253859. 4. Hart, Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific...

18. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    ... Slaymaker et al. made a variety of alanine substitutions throughout the groove in 32 separate Cas9 mutants...worries about off target effects in applied and/or therapeutic settings. Will combining the different mutations...

19. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    ... N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant to epidermal growth...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

20. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    ...literature searching and reading a regular part of your routine. Set a goal to read a certain number of papers ...though you may need to be on a network with an institutional subscription), archived on PubMed Central, or...