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  1. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ... break site (Figure 2A). The single molecule resolution of digital PCR allows classification of individual...total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA from a few thousand...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary to improve...multiplexing afforded by PTGs will improve the utility of CRISPR/Cas9 in new avenues of research. The ...

  5. Addgene: The First Twenty Years

    Type
    Blog Post
    ...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ...

  6. Antibodies 101: Fab Fragments

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    Blog Post
    ...improve localization accuracy when used in super-resolution microscopy by reducing the distance between the...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https...

  7. RaPID Detection of RNA-protein Interactions

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    Blog Post
    ...Unfortunately, using proteins as bait has limited utility for identifying novel RNA-binding proteins that...proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-...

  8. dTAG - You're it!

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    Blog Post
    ..., broad utility for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules...

  9. To Each HIS Own

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    Blog Post
    ...that of the protein of interest. A high imidazole elution buffer disrupts the interaction of the protein ... disrupt contaminant-metal ion binding without eluting the target protein and needs to be empirically ...

  10. All in a Twist: dsRNA

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    Blog Post
    ...responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount of siRNA...just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease, and much more...

  13. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post
    ... N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant to epidermal growth...and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource Pages  Resources at Addgene...

  16. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ...Agrobacterium Ti virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative...Resistance Genes Antibiotic Resistance: An Old Solution but a New Problem Plasmids 101: What is a Plasmid...

  18. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...Conditional gene-targeting in mice: problems and solutions." Immunity 48.5 (2018): 835-836. PubMed PMID: ...

  20. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    ...that you will need large volumes of lentivirus routinely, you may opt for simply increasing the number ...ultracentrifuge, PEG will precipitate all proteins in a solution, not just the lentivirus; consequently, this may...
Showing: 401 - 420 of 737 results