...approach are thought to be off-target effects and variable silencing efficiency. To address these shortcomings...screening method was limited in two substantial ways: variable silencing efficiency and labor-intensive processes...sufficient degree remain alive. This likely reduces variability in the RNAi output and trivializes downstream...

...The mycoplasmas enter the cell culture through various sources that are difficult to trace. These include..., PCR primers specific to the 16s RNA genes of various commonly infecting mycoplasmas are used. A broad...coughing in the lab. The human mouth contains a wide variety of mycoplasmas and these can enter the cell culture...

...and specificity. However, there are countless variables that impact an antibody’s affinity and specificity...very similar proteins. Additionally, they require variable expression of your target across samples - it ...it is hard to distinguish between specific, invariable expression and non-specific, background signal, ...

...vector for this step may save time as it reduces variability that may be encountered with DIO/FLEx vectors...off-target expression. Since recombination rates vary between every lot of AAV vector, a range of dilutions...rare for a methods section to include all of the variables that need to be accounted for (i.e. serotype, ...

...work. Alina Chan is a postdoctoral researcher at Harvard Medical School working on human artificial chromosomes...Science in Albany, NY, many tables were set up with various science demonstrations, and students could walk...engineering synthetic microbial communities at Harvard University to studying an evolutionary arms race...

...delivered and expressed in multiple somatic cells via various methods. Some of the more common delivery methods...cells [31] Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...training from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ ...

...lines, some of which are commercially available. Various proteomic and transcriptomic datasets can be used... confirm. As such, the approach used may need to vary according to the target, sample type, and available... J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, ...

...envelope plasmids are generalized and appropriate for varied cell types and systems. When planning your experiment...incompetent: Uses 3 separate plasmids encoding various HIV genes. Safer. Replication incompetent and always...This means that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses...9880. PubMed . Retroviruses. Coffin JM, Hughes SH, Varmus HE. Cold Spring Harbor Laboratory Press. Cold Spring...

...Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications (such as visualize...

...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...

...textbooks. I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the ...foundations of synthetic biology, DNA assembly, and various applications. I think these papers are good starting...

... feature step-by-step instructions to navigate various lab processes.  Our most recent protocol video ...feature interviews with real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their...

...for our audience of biological scientists from a variety of different fields. Exposure - With over 100,000... Network - Addgene will promote your blog on a variety of social media outlets meaning you’ll have the...

...spend some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his ...Seeding Labs Plasmid grant and is working on a variety of projects in Ghana.     Special thanks to our...

...a small start-up is very interesting. It’s very varied. There’s IP work to do, general corporate work,...is bringing experience and judgement to a wide variety of business decisions that are not “legal” at all...

...’s molecular biology work has focused on using various cloning methods and tools to construct chimeric...like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins. Dr. Duedu’s plans for these...

...throughput washing devices that are compatible with a variety of pipette tips. This is part of a cultural change...hazardous. Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...

...found. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...rush to give honest feedback to the manager for a variety of reasons. They may feel that giving feedback ...

...Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. ... expression of dCas9 and gRNAs whose expression varies depending on transgene design and delivery. They...

...prominence among all the noise. Scientists have a variety of metrics they can use to evaluate the impact ... this feature will draw attention to the great variety of plasmids in the repository. And last, but certainly...