We narrowed to 771 results for: Vars
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  TypeBlog Post...textbooks. I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the ...foundations of synthetic biology, DNA assembly, and various applications. I think these papers are good starting...
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  New Educational Resource: The Addgene Videos PageTypeBlog Post... feature step-by-step instructions to navigate various lab processes. Our most recent protocol video ...feature interviews with real Addgenies who have a variety of roles at Addgene. You’ll hear all about their...
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  Call for Guest BloggersTypeBlog Post...for our audience of biological scientists from a variety of different fields. Exposure - With over 100,000... Network - Addgene will promote your blog on a variety of social media outlets meaning you’ll have the...
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  Interview with Bart Newland, Addgene’s Newest Board MemberTypeBlog Post...a small start-up is very interesting. It’s very varied. There’s IP work to do, general corporate work,...is bringing experience and judgement to a wide variety of business decisions that are not “legal” at all...
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  Kwabena Duedu on Public Health, Biofuels, and Doing Science in GhanaTypeBlog Post...spend some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his ...Seeding Labs Plasmid grant and is working on a variety of projects in Ghana. Special thanks to our...
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  Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding LabsTypeBlog Post...’s molecular biology work has focused on using various cloning methods and tools to construct chimeric...like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins. Dr. Duedu’s plans for these...
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  Bringing Sustainable Practices to the Lab: InnovationsTypeBlog Post...throughput washing devices that are compatible with a variety of pipette tips. This is part of a cultural change...hazardous. Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...
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  Management for Scientists: Seeking FeedbackTypeBlog Post...found. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...rush to give honest feedback to the manager for a variety of reasons. They may feel that giving feedback ...
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  What's New in CRISPR - March 2020TypeBlog Post...Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. ... expression of dCas9 and gRNAs whose expression varies depending on transgene design and delivery. They...
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  Increasing Your Work's Visibility with Addgene: Citation, Search, and CollectionsTypeBlog Post...prominence among all the noise. Scientists have a variety of metrics they can use to evaluate the impact ... this feature will draw attention to the great variety of plasmids in the repository. And last, but certainly...
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  Mapping the 4D nucleome with CRISPR/Cas9TypeBlog Post..., and to understanding how nuclear organization varies across the life of a cell, or how organization ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...
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  Cre-ating New Methods for Site-specific Recombination in DrosophilaTypeBlog Post...drawbacks. First, the efficiency of Cre recombination varies for different constructs and cell types. Second...shown to be functional in cultured Chinese hamster ovary (CHO) cells, as well as in cortical neurons in layer...
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  New Viral Vectors - Fall 2024TypeBlog Post...Cre recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via ...Hub What is a Virus (and a Viral Vector)? AAV Variables that Matter...
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  Fluorescent Proteins 101: Fluorescent BiosensorsTypeBlog Post...biosensors, but keep in mind that there is a lot of variety in how biosensors work, and you should always check...process (e.g. enzyme activity) over time or under various conditions Localization within cells Fluorescence...
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  SunTag and Fluorescent ImagingTypeBlog Post... localization and expression may change across various conditions. However, this system is far from perfect...you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so...
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  Viral Vectors 101: Parts of the AAV Packaging PlasmidTypeBlog Post...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...
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  Genetically-encoded Sparse Cell Labeling - A SPARC of InnovationTypeBlog Post...length of the truncated attP site. The three SPARC variants, based on the length of the attP site, allow for...p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell types. Because the PhiC31 recombinase...
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  Hot Plasmids and Viral Preps - July 2021TypeBlog Post...in moderate thermophiles. These evolved SpCas9 variants have improved activity on NAG PAMS and reduced... Various Addgenies...
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  Five Questions to Ask If You're Deciding Between a Master's or PhD ProgramTypeBlog Post...Do you prefer being a generalist? Working on a variety of projects and in different fields of research...Everyday was the same and I was bored. I wanted more variety and more power to influence the direction of my...
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  Antibodies 101: Fc Effector FunctionsTypeBlog Post...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...