We narrowed to 532 results for: abo.3

...and best characterized of these is the Src homology 3 (SH3) domain family, known to be involved in cytoskeletal... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used...

...PMID: 21364278. PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene ...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

...10) and grow them in a small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

...ssRNA engaging one strand of a dsDNA molecule; the 3-stranded structure produced is known as an R-loop....power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

...synthetic biology tools for cyanobacteria. Figure 3: Assembly of plasmids using the Cyanogate Kit and ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

...PubMed PMID: 2839833. PubMed Central PMCID: PMC281709. 3. Tissue– and site–specific recombination in transgenic...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

...spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other species can recognize...our CRISPR 101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook Resources...

...transfection. The other (mKate2) is regulated in the 3’ UTR by four miRNA target sites and thereby serves... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...

...sequences more efficient and less risky.   Fig. 3: Development of a cleavage-free dCas9-based gene editor... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. ...

...protein expression and purification. Figure 3: The Penn State Ladder run on SDS-Page gels. A: individually... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...

...and complete screening in just 3 days? Thanks to Tyler Jacks’ lab, this 3-day goal is is now attainable...construction of a so-called “genomically recoded organism” (3) but its portability to other bacterial strains remains... the assembly of TALE repeats into a single-tube, 3 day process by utilizing pre-assembled trimers to ...but be sure to let us know if you'd like to write about your plasmids in a future blog post. No time to ...pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long Liu at Oxford University, we are...you plan to use GMAP for your experiments, think about depositing your new plasmids with Addgene to help...and context-dependent human fitness genes, the laboratory of Jason Moffat at the University of Toronto ...

...enter the cells so they can go to work. Figure 3: Inducing the double strand break and introducing ...the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining .... For a C-terminal tag, as shown in the example above, we inserted a linker and GFP tag at the end of ... and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into a plasmid backbone... Plasmids, plasmids everywhere To find out more about gene edited hiPS cell lines generated using the ...line. The editing strategy and design described above can be used to create similar donor plasmids for...microhomology-mediated end-joining (MMEJ). To find out more about gene edited hiPS cell lines generated using these...

...ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding affinity... time, when someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD...mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-crystalize...for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two Fab fragments...2019). Structural diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans...

...Always ask for references and follow up with 3-4 to hear about those clients’ experiences. Good coaches will...Individual Development Plan Learn a Little about The Academic Labor Environment from the Future of Research...research.  It helps to spend some time thinking about what your goals are for the coaching relationship...with someone you connect with. Talk to the coach about their “style” and make sure this is a style that...coaching group is Still Point Coaching. One nice thing about a group is that sometimes this offers the option...

...either as this also results in a generic invitation. 3) Connect with people you have really talked to The...include a note about where you met IRL (in real life) and perhaps what you talked about (or how good the... this rule, but not that many). I’m asked a lot about requesting connections to complete strangers for...have been able to tell when some start-ups were about to be bought by seeing certain groups of people ...using social media as a scientists Read blog posts about networking ...

...against the antigen bound to the plate. Figure 3: Overview of using Direct ELISA to quantitatively ...blog Learn about the advantages and disadvantages of using monoclonal antibodies. Read about other techniques... require two different antibodies. As mentioned above, it can also be used to characterize antibodies ...concentration of proteins in an ELISA well As mentioned above, the measurement of the concentration of proteins...

...endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015). 10.1016/j.cell.2015.09.038 Harrington... is smaller than the traditional SpCas9 enzyme (about a third of the size) and does not require a tracrRNA...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ... the lack of collateral cleavage). You can read about Cas7-11 in our blog post on its development and ...

...make up the disulfide bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally...Check out our post on Biotinylation to learn more about biotin and its use in molecular biology. Other Conjugates...perfectly blissful, productive lives without thinking about these chemical reactions. After all, there is an...ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...

...PMID: 24529477. PubMed Central PMCID: PMC4139937. 3. Anders, Carolin, et al. "Structural basis of PAM-...With this new found structural knowledge, in collaboration with the Joung Lab, they used targeted mutagenesis...edits in mammalian cells and may decrease worries about off target effects in applied and/or therapeutic...at Addgene. Resources on the Addgene Blog Read about Cas9 Homolog Cpf1 Read How Non-Homologous End Joining...

... The McMurray lab found that low concentrations (≤3 mM) of GdnHCl can functionally rescue multiple Arg-mutant...glycosylation (CDG) are a group of rare inherited metabolic diseases with severe and life-threatening consequences...understood.  In line with this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG...discovery led them to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential...