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We narrowed to 1,091 results for: addgene

Showing: 401 - 420 of 1091 results

  1. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep...

  2. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell...blog, we’ll walk you through how a scientist at Addgene uses Snapgene to confirm the sequence of a plasmid...designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list...sequences coming soon!  Additional Resources on the Addgene Blog How to verify your plasmid Check out our ...featured topic page Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular...

  3. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020   Additional resources on the Addgene blog Read about...essential research into COVID-19 (like here at Addgene!), and new databases are supporting their access...guide for external collaborations Resources on Addgene.org Browse our COVID-19 plasmid collection ...

  4. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...website, Help Center, or email us at deposit@addgene.org. ...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...resources at Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other...as plasmid yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile...

  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...

  7. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...We’re happy to introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business.... I think these are things that I can bring to Addgene -- that comfort with saying, “if we do this, then...

  9. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific...

  10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols...needed to expand the HEK293 cells. Check out the Addgene AAV Production protocol for more details. Pro tips...because I don’t have enough AAV. Compared to the Addgene AAV Production protocol, I take a lazier approach... the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more details...PubMed PMID: 18265393. Additional resources on the Addgene blog Find out what makes AAV a versatile tool ...

  11. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping ...planning on lounging poolside or swimming laps, Addgene has pooled libraries for any summer project. While...  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users: in an effort...effort to expand your gene delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why...projection neurons. Created with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses... viral vectors ready to use in your lab. Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ...

  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's Video Antibody Purification...of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and... are contained in (Randolph et. al., 2018). At Addgene, we ensure the efficacy of our antibodies through...Resources and references More resources on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

  13. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from Addgene ...PubMed PMID: 18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs ...for Gene Expression in Mammals Resources on Addgene.org Find AAV Packaging and Transfer Plasmids Here...

  14. Deck the Lab 2022!

    Type
    Blog Post
    ...Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will ...or Instagram with #DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have...

  15. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ... Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the...future. Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of ...inspiring research. Additional Resources on the Addgene Blog Learn How We Raised Money for Rare Disease...Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer Pages Learn about Global...

  16. Editor's Choice, July 2016

    Type
    Blog Post
    ... them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the... Science Communications Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on ...to read all of our excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse...

  17. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ... and have the biggest impact. We should know — Addgene has built its foundation on this idea! And we believe...high specificity and brightness, now available at Addgene!   {% module_block module "widget_58e2d882-2fb6...experimental requirements. Best-in-class collection at Addgene The GTA contains data on thousands of AAV enhancer...enhancer plasmids, which are all available at Addgene. This allows scientists to choose enhancers that may... or target a very specific cell type. However, Addgene has partnered with the AIBS to showcase the “best-in-class...Science. There are also more resources available at Addgene, including the Allen Institute for Cell Science...doi.org/10.1002/bies.202300044 Resources on the Addgene blog Plasmids 101: Gene, enhancer, and promoter...

  18. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with... recombinant phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie ...Protoc. doi 10 (2008). Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read... Tags for Protein Purification Resources on Addgene.org Find Synthetic Biology Plasmids Find Bacterial...

  19. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...conference format. Additional resources on the Addgene blog Read all of our blog posts about conferences...scientific conferences have followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended...conferences (TAGC and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility...

  20. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome One of those...the award from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the...iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes and other ...analyses.  “We believe that with this award from Addgene, we’ll be able to make big progress in elucidating...
Showing: 401 - 420 of 1091 results