We narrowed to 437 results for: ena

1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ...compared to wild type BirA. TurboID and miniTurbo both enable biotin labeling in 10 minutes without any issues...

2. Site Directed Mutagenesis by PCR

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   ...reduction: a method for analyzing the role of renaturation temperature in the polymerase chain reaction...

3. Using Phosphoserine to Study Protein Phosphorylation

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   ...discovery of a phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation...

4. Evolution of Lab Techniques

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   Blog Post

   ...Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia." New England Journal...

5. Thoughts on the Future of Research 2015 Boston Symposium

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   ...conversations like those at the FOR symposium and will enact their solutions as soon as they become actionable...

6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   ...Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species...

7. CRISPR 101: Off-Target Effects

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   Blog Post

   ...nothing to worry about all. Let’s consider several scenarios below for a CRISPR experiment which involves making...

8. Plasmids 101: Dimers and Multimers

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   ...Levene SD. Analysis of DNA topoisomers, knots, and catenanes by agarose gel electrophoresis. Methods Mol Biol...

9. Plasmids 101: Repressible Promoters

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   ...ethanol accumulates, it binds to the repressor, enabling it to bind the promoter and repress pADH1 activity...

10. PiggyBac-ing Through the Genome Editing Field

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    Blog Post

    ...technology, however, is that the transposase also enables the excision of the transposon in a completely ...

11. Finding Your Perfect Job After University

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    Blog Post

    ...and how I felt about the different positions to enable them to decide for themselves.  Here is my experience...

12. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ...emphasize in order to be selected in foreign labs.” Elena Grosso (Mentee IMP 2014-15, University of Valencia...

13. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ...should be made available for cell culturing and maintenance of the cell lines. Providing such an environment...

14. Important Considerations When Using AAVs

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    .... The Future What new types of research may be enabled by the use of AAVs? 1) Combined functional and ...

15. Quick Guide to All Things Lentivirus

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    ... the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence of Dox. In this...

16. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...systematically test the potential causes of these phenomena by analyzing the plasmid and viral DNA sequences...

17. A Guide to Getting Started in Undergrad Research

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    Blog Post

    ...: managerial duties ex: order materials, lab maintenance Administrative assistant: assists PI with admin...

18. 9 tips for a successful postdoctoral experience

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    Blog Post

    ...? What are your controls? Will these controls enable you to interpret your results? Will the experiment...

19. Sequencing Options for CRISPR Genotyping

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    Blog Post

    ...PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling of off-target cleavage by...

20. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post

    ... Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian...