Targeted Mutagenesis with EvolvR
Type
Blog Post
...be used to map protein-protein interactions, investigate non-coding segments of the genome, or engineer...
We narrowed to 536 results for: invs
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15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...open up a new direction for the project. I was invited to participate on a panel about “Social Media for... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...protomoting or repressing protein expression. Methylases involved with these processes (for example Dam and Dcm ... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...project. README: The README.txt document is invaluable. In the top directory of every experiment, immediately... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post...the initial cut, the next steps in the process involve repairing chromosomal DSBs. It is important to ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...also available at Addgene, enabling systematic investigation of the cellular consequences of repressing or... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog Post...colleagues you know will attend the conference. Invite others to join you. A fun, memorable meal is a ... -
Summer SciComm: Preprints
TypeBlog Post...details that you couldn’t fit on your poster, or invite attendees to read and comment online! Want the ... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...problem solving that I did in the lab has been invaluable in my more recent positions outside of the lab... -
Antibodies 101: Conjugation
TypeBlog Post...However, there are certainly other approaches that involve modifying the antibody sequence or that rely on... -
Click Biology: How to Click and Play to Explore Biology
TypeBlog Post... post was written by Professor Mark Howarth, investigator at the University of Cambridge. His research... -
Addgene: The First Twenty Years
TypeBlog Post...hear it all the time, but Addgene is a totally invaluable resource. I really appreciate the work you do... -
Visualizing Protein Turnover In Situ
TypeBlog Post...synthesized proteins in situ. I was privileged to be invited to learn from them at the MPI in Frankfurt am Main... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post... in which the hM4Di was fused to mCherry. To investigate the localization of the hM4Di, we used primary... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...healthcare system was $35.2 billion (or $140 per $1 invested in the trial). The goal of gendered innovations... -
The Future of Research Symposium Boston 2015
TypeBlog Post...workforce, in particular about postdocs. Among other involvements, he helped to establish grants through the Sloan... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...recognition sites that flank their DNA fragment in an inverse orientation. This allows for multiple DNA components... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post... give feedback on the application. Make sure to invest time and consider the reviewer's comments. Whether...