We narrowed to 537 results for: invs

1. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting...

2. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ...open up a new direction for the project. I was invited to participate on a panel about “Social Media for...

3. AAV Q&A with Tim Miles

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   Blog Post

   ...While we have not tried it, it might be worth investigating for both Q6 & Q7.For epithelial cells of the...

4. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   ...protomoting or repressing protein expression. Methylases involved with these processes (for example Dam and Dcm ...

5. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   ...project. README: The README.txt document is invaluable. In the top directory of every experiment, immediately...

6. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...

7. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ...the initial cut, the next steps in the process involve repairing chromosomal DSBs. It is important to ...

8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

   ...also available at Addgene, enabling systematic investigation of the cellular consequences of repressing or...

9. How to Make Friends and Meet People at a Scientific Conference

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   Blog Post

   ...colleagues you know will attend the conference. Invite others to join you. A fun, memorable meal is a ...

10. Neurodegeneration Plasmid Collection

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    Collection

    ...several sources (Chan Zuckerberg Initiative, MedGen, ClinVar) and is meant to be representative but not exhaustive...

11. Addgene: The First Twenty Years

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    Blog Post

    ...hear it all the time, but Addgene is a totally invaluable resource. I really appreciate the work you do...

12. Antibodies 101: Conjugation

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    Blog Post

    ...However, there are certainly other approaches that involve modifying the antibody sequence or that rely on...

13. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...problem solving that I did in the lab has been invaluable in my more recent positions outside of the lab...

14. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ... in which the hM4Di was fused to mCherry. To investigate the localization of the hM4Di, we used primary...

15. Visualizing Protein Turnover In Situ

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    Blog Post

    ...synthesized proteins in situ. I was privileged to be invited to learn from them at the MPI in Frankfurt am Main...

16. Summer SciComm: Preprints

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    Blog Post

    ...details that you couldn’t fit on your poster, or invite attendees to read and comment online! Want the ...

17. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post

    ...healthcare system was $35.2 billion (or $140 per $1 invested in the trial). The goal of gendered innovations...

18. The Future of Research Symposium Boston 2015

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    Blog Post

    ...workforce, in particular about postdocs. Among other involvements, he helped to establish grants through the Sloan...

19. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ...recognition sites that flank their DNA fragment in an inverse orientation. This allows for multiple DNA components...

20. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post

    ... give feedback on the application. Make sure to invest time and consider the reviewer's comments. Whether...