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We narrowed to 850 results for: met

Showing: 401 - 420 of 850 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete...Quantitative assessment of fluorescent proteins." Nature methods 13.7 (2016): 557. PubMed PMID: 27240257. PubMed... expressed inositide binding protein domains." Methods 46.3 (2008): 167-176. PubMed PMID: 18930153. PubMed...proteins to study mRNA trafficking in living cells." Methods in cell biology 85 (2008): 273-292. PubMed PMID...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...cassette. This leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes...leave any undesired scars in the chromosome. These methods roughly fall into two categories: those that rely... a sucrase that converts sucrose into a toxic metabolite. Cells that have undergone a double crossover...identify double crossovers. This can be an efficient method to generate precise, unmarked mutants, but it frequently...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...helpful. I set out to make something practical and accessible. If there's something you come across that is...The second question is whether there is chalk. Sometimes. It can also be a slide deck, markers, etc. Chalk... What is the big question? This should be something that anyone could relate to, that people would...

  4. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...approach combines the overlapping and trans-splicing methods. The AAV encoding part A of the transgene contains...of the transgene have been seen with the hybrid method. While these fragments are likely non-functional...Frischmeyer et al., 2002). How to pick a split AAV method While the hybrid dual vector system has a slight...Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140...

  5. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy... has recently been used to study a matrix metalloproteinase: López-Pelegrín, Mar, et al. "A novel mechanism...mechanism of latency in matrix metalloproteinases." Journal of Biological Chemistry 290.8 (2015): 4728-...Christopher Marx's lab has engineered and validated a new method called FREQ-Seq. This strategy allows scientists...

  6. Finding Your Perfect Job After University

    Type
    Blog Post
    ...failure and problem solving. I believe that is something lacking during degree courses; they never really...the company was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on...situation, communication between departments was sometimes difficult and the solution attempted was more ...solved with good management practices, but can sometimes go unnoticed at large organizations. Another downside...

  7. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...Surprisingly, the team noticed that this editor sometimes introduced C → G edits at certain positions within...multi-step cellular repair pathway that is known for sometimes causing double-strand breaks (DSBs). The Fei-Long...common intermediate of CGBE activity, and they are sometimes processed by error-prone non-homologous end joining...context preference predictable by machine learning methods. Nature Communications, 12(1), 4902. https://doi.org...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... database. Previously developed genome editing methods using nucleases and base editors have the potential...less constrained by PAM availability than other methods. The PAM-to-edit distance can be over 30 base pairs...can be used with any of the PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc. PE6: Specialized.... Although the addition of the RNA pseudoknot sometimes altered epegRNA expression compared to pegRNAs...

  9. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...and methods section. This section will vary slightly depending on the application — flow cytometry, western...  Figure 4: A screenshot of the materials and methods section for the data report shown in Figure 3. ...

  10. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...mechanism of matrix metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’...genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903. PubMed...

  11. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...the fusions had some negative effect on these parameters. IT5 and IT6 displayed good binding efficiency...epitopes for innocuous protein tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte...protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed PMID: 23435261. Resources...

  12. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...timelines Be a mentor in a formal program Start something (a journal, a science lunch club, a biotech club...division of labor Serve on a committee and do something big like plan a conference Recruit many supporters...departments want to be able to tell that you know about something and are passionate about it.   Maintain a content-useful...

  14. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... protein, PSmOrange. Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids...photostable photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids ...

  15. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...were pleasantly surprised by the efficiency of the method. One observation was an inverse relationship between...sites within a single gene using paired DSBs. This method allows for deletion screening by conventional PCR... and Bauer lab's related paper describes their methods for CRISPR design, cloning, and delivery, as well...

  16. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...by its lower resolution. The combination of this method with superresolution microscopy may improve the... a concern. Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum...organization. This fluorescent CRISPR/Cas9-based method is a great step forward in live cell imaging of...

  17. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...selecting for the loss of a gene product - usually something toxic. This gene is found on the original plasmid...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...levansucrase that converts sucrose into a toxic metabolite in gram-negative bacteria. Plating on sucrose...

  18. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...periodically beat their tails left and right in a symmetrical motion to move forward. This behavior is not ...Kolmer-Agduhr cells was sufficient to trigger the symmetrical tail movement unique to spontaneous slow forward... photostimulation in order to drive the full symmetrical response.  The function of Kolmer-Agduhr cells...

  19. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...residues (or more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His...His fusion proteins to be purified by single step metal affinity chromatography. Purification of His fusion...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

  20. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...Sequence tool.   DNA sequence manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed...backbone you need for different types of cloning methods, such as restriction enzyme cloning, Gibson assembly...Ligation Calculator: This tool works for any cloning method. You can select from five different insert:backbone...
Showing: 401 - 420 of 850 results