...plasmids for metabolic enzymes and pathways.... Plasmid Collections Synthetic Biology Metabolism Synthetic ...Synthetic Biology: Metabolism SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome...Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains...collection of synthetic biology plasmids related to metabolic pathways and components. Examples Include: Biofuels...Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword or sort ...

...MAP2K; Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase MLST8 ...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related...TIAM TIAM1 TIAM2 T-cell lymphoma invasion and metastasis TP53 Tumor protein p53 TSC TSC1 TSC2 Tuberous...

...TCCAAGCGCGAAAACCCCggatggtgaggagcaggTACTGGCCCGGCAGCGA MET TAL2326 & TAL2327 TTATTATTACATGGCTTTgccttactgaggcttcATCTTGTCCTCTGGTCCA...Joung Lab recently used their high-throughput FLASH method to construct a large series of engineered TALENs...

... M6PR-bio-His 52022 MET-bio-His 52024 SORT1-bio-His 52025 CD36-bio-His 52026 ARMET-bio-His 52027 CCL5-...

...Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods Multiplex Assays ...Antibody Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows... through the indirect detection method. In the direct detection method, the signaling molecule is conjugated...Antibody Applications - Quantification Methods Quantification methods, such as western blots or ELISAs, are...output measured. This method can be performed using the indirect detection method via a conjugated secondary...sample. Controls for quantification methods Quantification methods should be run with: a positive control...included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP) and chromatin...

... Temperature Genetically encoded ratiometric fluorescent thermometer A highly-sensitive genetically encoded...sensors for metabolites like fructose-1,6-bisphosphate , cyclic di-GMP , or S-adenosyl methionine . Reporters...Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small Molecules Neurotransmitters Cell Physiology...; these are noted with the viral service icon . Metal Ions Target Description Article PI Calcium jGCaMP8...neuronal populations and microcompartments. Nat Methods. 2019 Jul;16(7):649-657. Douglas Kim , GENIE Project... with large dynamics and high sensitivity. Nat Methods. 2023 Jun;20(6):918-924. Yubin Zhou Calcium Twitch...bioRxiv 2023.10.12.562058. Robert Campbell Calcium Ratiometric calcium biosensors from nested reporters of green...

...Lindquist 15589 MET FLAG46QDProCFPp303 HTT FLAG, CFP MET1 Huntington's Susan Lindquist 15590 MET FLAG72QDProCFPp303...FLAG, CFP MET1 Huntington's Susan Lindquist 15591 MET FLAG103QDProCFPp303 HTT FLAG, CFP MET1 Huntington's...FTD Ahmet Yildiz 159719 pOmnibac_ZZ_linker_ybbR_Tau MTBD MAPT ZZ, ybbR polH Parkinson's, FTD Ahmet Yildiz...Carlos Igual 89925 Profiline PFN1 T7 ALS Guillaume Romet-Lemonne 92200 pINDUCER20-delta-Syn SNCA Myc TRE2...

..., cell viability, and tumor metastasis. Currently, the most popular method for conducting genome-wide ... to conventional methods for genomic purification, CRISPR-based purification methods are more straightforward...1.1). Phage-assisted continuous evolution (PACE) methods resulted in xCas9 3.7 which has 7 mutations found...inducing double-strand breaks by modifying the methylation state of cytosines in a gene’s promoter or by...by inducing histone acetylation or demethylation. CRISPR epigenetic tools are specific for particular ... libraries containing Cas9 are the most popular method for CRISPR screening, they are not suitable for...hybridization (FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living ...

...difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides...combinations A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 May;8(5):409...an overview of several different reprogramming methods with the goal of helping readers choose a strategy...strategy suited to their research. Once you know which method you would like to use, browse Addgene's collection...cell into another via transdifferentiation. This method of cell lineage conversion can be faster, bypasses... stem cells (iPSCs). Sort the table by delivery method, species, or PI and then select the article link...link to find more information. Delivery Method Species Description Article PI Lentivirus Human Expression...

...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...pOpen-NcoIM M.NcoI DNA methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red...

...Activation Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini...Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann 3rd ~10...Knockout Mouse Yusa 3rd 5 90,230 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy...169082 Knockout Mouse Turner N/A 10 13,500 Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library 123361...Inhibition Yeast Tavazoie N/A 6-12 51,681 Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~...Knockout Mouse X.S. Liu 3rd 5 5 10 49,252 Human Metabolic Gene CRISPRa sgRNA Library 187080 Activation Human... Human Birsoy 3rd Varies 32,460 Mouse Metabolic Gene CRISPRa sgRNA Library 187081 Activation Mouse Birsoy...

... expression in rEcoli XpS in the Methods section link below (Methods for iSPI, rEcoli XPS, pSerOTS-C1*...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al, Nat. Biotech. 2018). This phosphosite...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library...resource described in (Gassaway et al., Nature Methods 2022) is adapted from the synthetic human phosphoproteome...phosphorylated positions for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite...phosphosite localization algorithms, or any method confounded by phosphosite ambiguity. Rinehart Lab Improved...Paulo JA, Rinehart J, Huttlin EL, Gygi SP. Nat Methods 2022 Nov;19(11):1371-1375. doi: 10.1038/s41592-...

... and 6.2 recommend an efficient method for DNA extraction, any method for genomic DNA isolation may be...and genetic elements. Here we present a simple methodology for CRISPR design, cloning, and delivery for ... Anneal in a thermocycler using the following parameters: 37 °C for 30 min; 95 °C for 5 min and then ramp...samples in a thermocycler using the following parameters: Cycles 1-20 (37 °C for 5 min, 20 °C for 5 min...system. NOTE: Alternatively use another transfection method such as cationic liposome-based transfection. Optimize...proliferated once macroscopically visible with ~2 mm diameter. Primer Validation and Screening for CRISPR/Cas9...samples in a thermocycler using the following parameters: 95 °C for 15 min, 35 cycles of (95 °C for 30...

...criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2 ). MECP2 Rett syndrome...is not clear, however, the ability to bind to methylated DNA and recruit known co-repressors or other ...NID demonstrating the importance of binding to methylated DNA and the recruitment of the NCoR complex for...below: Base Editing RNA Editing CRISPR-based DNA methylation editing system - Shawn Liu and Rudolf Jaenisch...clinical trials (Link opens in a new window) HRV Metrics Toolbox - algorithms for data analysis to calculate...caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet . 23, 185–188....new window) PMID: 16905679 Cuddapah et al. 2014. Methyl-CpG-binding protein 2 (MECP2) mutation type is ...

...Hang 163915 pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial TAG...200226 pMega-MaFRSA PylRS M. alvus meta-trifluoromethylphenylalanine Bacterial TAG Alanna Schepartz 31186...David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl...ATG David Tirrell 89189 pMaRSC methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl...TAA Wenshe Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine synthetase M. jannaschii family...p-iodo-L-phenylalanine Bacterial TAG Dieter Söll 51401 pAM1 NLL-MetRS E. coli azi-donorleucine (Anl) Bacterial ATG David...

...Expression mAmetrine 406 526 26 6 0.8 hr Monomer (A206K) pBad-mAmetrine - Bacterial Expression mAmetrine-N1 -... these proteins, see: Cranfill et al. , Nature Methods 2016 Jul;13(7):557-62. FPbase : A database for ... - Mammalian Expression mAmetrine-C1 - Mammalian Expression LSSmOrange 437 572 23 5.7 2.3 hr Monomer pLSSmOrange-C1...Jump to Top References: Cranfill et al. : Nature Methods July 2016, Vol. 13(7):557-62 Olympus Confocal :...December 2007, pp. 4247-4260 Shaner et al. : Nature Methods, Vol.5 No. 6, June 2008, pp. 545-51 Shcherbakova.... 24 No. 5, pp.577-81 Shcherbo et al. : Nature Methods, September 2007, Vol. 4 No. 9, pp. 741-6 Shemiakina...2005, Vol. 10 No. 1, p. 14003 Chu et al. : Nature Methods, May 2014 Vol. 11 No. 5, pp.572-8 Yu et al. : Nature...

...lysate method. Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios...Overview of Addgene's viral production methods and quality control for lentivirus and AAV. Viral...Production Viruses are generated using standard methods that have been optimized for each specific virus...Details about our production protocols, titering methods, and quality control are described below. AAV Production...higher than those achieved using the standard qPCR method. Titering by the University of Pennsylvania Vector...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

...including histone acetylation/demethylation, and cytosine methylation/demethylation....p300 histone demethylation by LSD1 cytosine methylation by DNMT3A or MQ1 cytosine demethylation by Tet1 These...

...TALE fusions with the histone demethylase LSD1 (Lysine specific demethylase) made using these plasmids along...with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation at the target...Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam... Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC...(assembled using the REAL, REAL-Fast, or FLASH methods) for expression as TALE-LSD1 fusions. It was recently...

...FLASH assembly methods can be found on the TALengineering website . All three of these methods yield TAL effector...arrays assembled using the REAL, REAL-Fast or FLASH methods. TALengineering... (assembled using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as TALE transcriptional...artificial transcription factors ( Maeder et al., Nat Methods 2013 ). All expression vectors listed below have... of the assembly are different among the three methods, the final products are the same). The publicly...