We narrowed to 779 results for: mos
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TypeBlog Post...with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics... be very informative. Spoiler alert: HeLa is the most common contaminator line. For those wanting to ...
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SunTag and Fluorescent Imaging
TypeBlog Post...display protein aggregation, it’s recommended for most imaging applications. What can you do with SunTag... experiments in their paper, and so can you! In almost any case you would use a traditional FP fusion,... -
Tips for Improving Your Next Manuscript
TypeBlog Post...Montgomery, or Louis Miller led these sessions. The most beneficial aspect of this course was breaking up...works. 4. Practice writing everyday Overall, the most important instruction the classes taught me was ... -
Plasmids 101: What is a plasmid?
TypeBlog Post...advancing the field. What is a plasmid? At their most basic level, plasmids are small circular pieces ...that replicate independently from the host's chromosomal DNA. They are mainly found in bacteria, but also... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...levels of an organization and is also one of the most common criteria required for a promotion. Yet little...fulfilling the requirements of the current task. Most of us start as doers, some become managers and some... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...Monday night represented engineering first and foremost, from the custom software used to design complex...Book" for this month's Addgene Book Club. I read most of the book on my way to the meeting, and its history... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/Cas moieties carry out these...Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org/wiki/File:Crispr.png ... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post... on Twitter if you missed our live updates! My most popular tweet from the conference is shown above...nucleases, but with a session on DNA repair. What stuck most in my mind from that session were words from Eric... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...interface with every possible option, we place the most important controls front and center, and make specialized...features. This algorithm enables one of SnapGene’s most popular aspects - its ability to annotate a raw ... -
Summer SciComm Series: Master of SciComm
TypeBlog Post... on the Leith river. Photo courtesy of Leonardo Ramos. I enrolled into a summer school course at ...confluence of science and the world it hopes to change. Most researchers are aware of the importance of science... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...widely used in synthetic biology, biotechnology, and almost every microbiology lab,” says Di Ventura. It relies...still reached the level of expression needed for most applications. Figure 1: With blue light... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...the time. It is 1.5 to 3 times brighter than the most commonly used GFPs and YFPs. Its excitation maxima...EGFP does. Find mWasabi plasmids here! One of the most notable things about these proteins is that they... -
Summer SciComm: Science Storytelling
TypeBlog Post...that they then tuck the science into. There are almost limitless storytelling options, and they all can...these storytelling devices, we sometimes forget the most important thing about science: It’s cool. Like bowties... -
Plasmids 101: Origin of Replication
TypeBlog Post... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...that create a toxic product. The E. coli strain Most E. coli strains can be used to propagate plasmids... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog Post...Breakdown of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids...Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career Posts... -
Another Pathway into Cells: iTOP
TypeBlog Post...introduce a gene of interest into primary cells, so most primary cell lines require viral infection. A new...a new idea, but it comes with a few caveats. The most common protein-only transduction strategy is to ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...had to think creatively so as not to develop the most obvious strategy which also could have been proposed... In the end, the students had fun while applying most of the concepts required by the course and some ... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...PMCID: PMC3574576. 7. S.-L. Lai, T. Lee, Genetic mosaic with dual binary transcriptional systems in Drosophila... for transgene expression, lineage tracing, and mosaic analysis., Cell 141, 536–48 (2010). PubMed PMID... -
25 Work From Home Tips for Scientists
TypeBlog Post...bench scientist who began working remotely recently? Most of us at Addgene began working from home last week... negative data or publish brief, novel findings. Most Micropublications contain one figure. If you aren... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...Hernández Sánchez from Crowdfight. In March 2020, while most of the world was locked down, a South African company...competitiveness of cutting-edge research, is still one of the most valuable features of modern science in our view....