We narrowed to 928 results for: mpl

...Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example Article Number...However, determining this exactly is difficult. For example, it is very plausible that science published in...research our adjustment controls for them.   So, for example, in 2010, 10 percent of the papers with Addgene...factors that could still be controlled for. As an example, plasmid-related areas of science could be growing...

...require authors to use specific PID formats. For example, several journals like eLife, Nature, and Cell ...as Identifiers.org and N2T.net. To help authors comply with journal policies, Addgene now provides an ...these PID formats are new to you, we’ll go through examples to highlight the difference between them using...identifier and RRID formats. Let’s go back to our example plasmid, lenti-Cas9-VQR-Blast. If you scroll down...multiple PIDs separated by semi-colons. For our example plasmid it will read “lenti-Cas9-VQR-Blast was ...

... bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR technology becomes increasingly...using it to investigate potential therapeutics for complex genetic diseases. So we’re revisiting this post...look at CRISPR’s potential for use in treating a complex genetic diseases, Alzhiemer’s disease (AD).  Troy...diseases with real phenotypic effect.” “It’s a very simple technology,” Doudna further explained. “That’s ...Gene Therapy: Applications, Limitations, and Implications for the Future.” Front. Oncol. https://doi.org...

...to target cells as a pooled library, which will amplify each TF to determine how its presence influences...processes. For help with library transformation and amplification, refer to the Zhang lab’s previously published...the specific dysregulation in the disease. For example, very few diseases manifest as a single mutation...expression dysregulation event. Diseases are often complex, with multiple layers of dysregulation from either...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library...

...equivalent of crossing off days on the calendar after completing your writing goal, which can serve as positive...graph of which days of the week you most often completed your habit is useful for figuring out when to ...makes suggestions on how to make your writing less complex. While I don’t think you should accept all of the...jargon in your writing. While it’s not possible to completely avoid jargon, this tool can help you avoid overusing...tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique...

...was easy to find people who were interested. For example, our journal club discussion on Kevin Esvelt’s ... safety training prior to getting started. For example, Timothy Stiles joined BosLab last year with a ... space to perform early-stage experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab... getting the community to collect various soil samples, extract DNA, sequence it, and analyze the data... characterize the microbiomes of the different samples. The goal is to not only learn something about ...

...particular reference sequence if necessary. The example plasmid on the right has a total size of 7.3kb,.... Avoid star activity. Some endonucleases (for example BamHI) are capable of cleaving sequences which ... The glycerol in the buffer will make sure your sample settles in the gel well and the dyes provide a ...you to interpret the bands that you get in your sample lanes. Choose your ladder based on the expected...approximate the DNA mass of comparably intense samples of similar size. Run the gel at 80-150V until you...

...for plasmid replication in mammalian cells, for example, and a toxin that kills bacteria may not have any...Selection methods, described below, are typically employed when creating stable cell lines and are not used...plasmid selection. Instead, reporter genes are often employed to easily monitor transfection efficiencies and...antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral EBV or SV40...HeLa, NIH3T3, CHO, COS-1, 293HEK, Jurkat T cells Complexes with DNA; causes strand scissions 100-400 ug/mL...

...just how much things have improved, check out the example below. Our old map is on the left while the SnapGene...on to learn more about the improvements we’ve implemented and how they’ll make it easier for you to find... next cloning experiment early on. Beyond these simple but powerful display improvements, when you click...or ORF to learn more information about it. For example, if you hover over the ampicillin resistance gene...software development teams for working tirelessly to implement these improvements. Let us know what you think...

...cancer precision medicine seems so simple! Take a patient’s tumor sample, use cutting edge genomic technologies... wild type, would be extremely helpful as a complementary approach. Also, in principle, these experiments...cancer phenotypes. These assays were designed to complement the one assay per gene approach that has been...genes. These should be used to create ever more complete “look-up-tables” of mutant function that could...

...to physically set up in the lab, RNAi is often completely ineffective or results in low penetrant phenotypes...low-cost, cell-free detection assay that can be completed without special equipment. What’s more, detection... to be easily concentrated. Viral RNA is then amplified isothermally, and detected through a toehold switch...encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer....modifying the sequence of the toehold switch. For example, Green has also developed toehold switches to detect...

...lab or pivot to a managerial role. For those contemplating the leap into management, the journey can be...redefine the value I could bring to potential employers. Over time, I discovered that I not only enjoyed...motivations, strengths, and learning styles. For example, during my time as a lab scientist, I worked with...foster a cohesive, productive environment. For example, in my current role managing a cross-functional...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals...

...stable host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a...processes. The food processing industry, for instance, employs psychrophilic enzymes for milk processing, fruit...Additionally, the high activity of these enzymes implies that even a minute quantity can significantly reduce...

...endless ways and everyone finds their own. Here, I simply outline what works for me, and I hope that it is...experiments can be found in each folder. This can be a simple .txt document. Record the name, date, and location... work on multiple projects in a given day. For example, instead of naming a file “output” name the file...track of your exact changes. Alternatively, you can simply make a note in your methods section about the edit...result was, so try not to do this. These are the simple rules that I try to follow in my own research. ...

... Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation of endogenous... assemble a synthetic transcription activation complex consisting of multiple distinct effector domains...with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier by the Zhang lab for...sam.genome-engineering.org/. Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu H, Ran...transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, ...

...strands take part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...directed toward AHA. Now the rolling circle amplification could only take place if the two antibodies ... observation of individual endogenous protein complexes in situ by proximity ligation." Nature methods...

...where one of the X chromosomes is partially or completely missing in women. Bicuspid aortic valve (an aortic...translate those signals into cellular responses. For example, GPCRs and their downstream G protein partners ...with an emphasis on oligomerization, signalling complex assembly, and localization to the nucleus,” says...at McGill University.  These protein-receptor complexes consist of many parts that work together on the...gene, which codes for G𝛃1 subunits of G protein complexes, lead to a rare type of developmental delay, and...

...based on size. Whether higher molecular weight complexes are detected will depend on the homodimer affinity...During the experiment, the solution is diluted, complicating the interpretation. Because of this, these methods...detecting homodimerization tendency. This assay employs a peptide derived from cytochrome p450, CytERM,...will be problem-free. In figure 1 we provide an example of the OSER assay with a number of fluorescent ...organisms that are engineered can be shared to simplify the comparison by others. Together, we can build...

... that actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting ...take up your insert. But exactly how does ccdB accomplish this? Let’s start with a brief history of the...system contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of...interest.   CcdB resistant E. coli strains complete the system But ccdB alone isn’t the only key to...protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3):735-45. PubMed...

... channels and pumps in developmental biology.  Examples of some ion-channel and ion-pump encoding genes...genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction proteins cx43...also modulating bioelectric states in vivo. For example, the Levin lab from Tufts University has used Arch...sensitive fluorescent voltage indicator with near-complete desensitization in continuous light. ArcLight ...extremely exciting emerging field, with many implications for regenerative medicine, evolution of body...