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Showing: 401 - 420 of 758 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...marker like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we...recombination using a repair template. These templates can include loxP sites to create conditional floxed alleles...

  3. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...are needed to optimize yield. You might want to include an extra wash step or use a different strain. For.... Look out for carbohydrates Certain strains, including HB101 and derivatives, release large amounts of...

  4. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...terrific getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs...regions” filter unchecked so that these regions are included in the search to maximize the alignment length...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...

  6. An Introduction to Adenovirus

    Type
    Blog Post
    ...– it makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and...which have fragments of the AdV genome in them, including the E1 region). The space in the viral particle...

  7. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...will simply express from whatever promoters were included in the vector (or are nearby in the host genome...

  8. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...the primer sequences. Multiple plasmids can be included in a single PCR, for example for subcloning genes...vector. The new codon and homologous region can be included at the 5’ end of either primer. Subcloning - To...

  10. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have been reported...al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated purification of a specific genomic...

  11. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... to a new plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix...in the detection of microbes. Some applications include measuring viable probiotics and detecting circulating...

  13. Plasmids 101: Protein tags

    Type
    Blog Post
    ...accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, ...combinations, though frequently those combinations still include at least one element from the original TAP tag....

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...target the ubiquitin E3 ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein...mScarlet-I3 that were codon-optimized for C. elegans and included artificial introns to improve expression. When...

  16. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...method can be used for an array of applications including clinical diagnostics (e.g. pathogen or virus detection...Cas12a is initiated. Surrounding trans-ssDNA, including the ssDNA-FQ reporter are subsequently degraded...

  17. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... works on our site. For Addgenies, development includes everything from tracking plasmids from the moment...then go back to this area later and tidy it up. Conclusion While none of us are life science majors, we ...

  19. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...show your enthusiasm for getting the position and include a brief summary of why you are a good applicant...taught for five years at [name of the university]” Include keywords from the job description to help the hiring...

  20. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...to the total number of viral capsids (which can include empty capsids that are devoid of a viral genome... et al., 2010). In addition to the AAV RSM, we include a second AAV reference sample of known titer, which...
Showing: 401 - 420 of 758 results