We narrowed to 755 results for: rest
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TypeBlog Post...decision. In the open-access Data Hub, researchers interested in the Addgene antibody collection can look at... selecting an antibody! We encourage everyone interested in the Addgene antibody collection to use and...
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A Scientist's Guide to Ebola
TypeBlog Post...Even that grim estimate might prove to be an underestimate. Given all the excitement and speculation, we...ongoing outbreak from the CDC and WHO. - For anyone interested in EBOV’s past, present, and future, don’t miss... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...commercial kits you will adopt for the extraction. Restriction enzyme based approaches can require several hundreds...cloning grade DNA for direct cloning of your gene of interest into a plasmid backbone. Transfection grade... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog Post...Citing Article" - such as cloning their gene of interest into a previously deposited vector - then you'...locate other papers that have used your plasmid of interest, which may help you identify relevant protocols... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...trainees at all. For grad students and postdocs interested in taking matters into their own hands, there...I am happy to work with any group of trainees interested in designing career exploration programs or peer... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post... from your sequencing runs. When you run a restriction digest on a gel you always include proper controls...primer at least 50bp upstream of the sequence of interest. 5. Use a silica spin column for purification ... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post...to be used as research tools is particularly interesting. Although humans have known about bioluminescent...deciding which one is right for you, so if you’re interested in learning more about fluorescent proteins, ... -
Your Top Requested Plasmid in 2016!
TypeBlog Post...this plasmid has a U6 promoter followed by BbsI restriction sites into which you can clone your 20 nucleotide...can use to express both SpCas9 and your gRNA of interest in a variety of mammalian cell lines. Alternative... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The most requested...next Top 10 list. 10 years of plasmid sharing Interested in more facts about Addgene's 10 years of plasmid... -
Let There Be LITE Plasmids
TypeBlog Post...can be applied non-invasively, its use is not restricted to cultured cells but extends to freely moving...plasmids have clear application to anyone with an interest in neuroscience, their use is by no means limited... -
Editor's Choice, August 2016
TypeBlog Post...discussed further in this informative post. Interestingly, this technology may make some uses of Site ... Software Engineer Kris Shamloo wrote a very interesting post about how we develop our in house lab management... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...screen for pathways that confer any phenotype of interest, not just drug resistance,” he said. “It could...contacted by investigators all over the world who are interested to use our libraries to do things - sometimes... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...characteristics and ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...of AAV plasmids will have technical or legal restrictions that make them ineligible for use in this service...consideration will have a banner on their material page. Interested scientists can expand the banner to select their... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...Of course, if you’re interested in using IBEX, you’re probably also very interested in having high-quality... -
What's New in CRISPR - September 2019
TypeBlog Post...double-stranded DNA break at the genomic loci of interest and on either side of the cargo (ex: fluorescent...the double-stranded break in the genomic loci of interest. As the universal sgRNA is not predicted to target... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...specifically isolate DNA that is bound by a protein of interest. First, nuclei from tissue or cell culture are...with a primary antibody against the protein of interest. The Protein A fused MNase is then added and Protein... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...works to meet the individual needs of schools interested in using eScience Labs as part of their curriculum...Learn more about alternative science careers here Interested in creative science education tools? Check out... -
Antibodies 101: Affinity Tags
TypeBlog Post...added to the N or C terminus of your protein of interest. Many of these tags also have commercially available...makes it amenable to being left on your protein of interest, especially if downstream antibody detection is... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...conditions without necessarily modifying the gene of interest in any way. For example, if green fluorescent ...situations. Tissue-specific genes – the genes of interest may not be expressed in the tissue/cell line you...