...transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the...plasmids that express the reprogramming factors of interest. The advantages of this method are that it’s relatively...KLF4 LIN28 Check out these plasmids if you’re interested in Tet-inducible lentiviral expression of OSKM...

...Diagnostic Restriction Digest Diagnostic Restriction Digest You may also like... Restriction Digest of ...another. A diagnostic restriction enzyme digest takes advantage of the fact that restriction enzymes cleave ... your plasmid of interest (see below for recommended amounts) Appropriate restriction enzyme (see manufacturer's...Orientation by Restriction Digest If you clone an insert into a vector by a single restriction enzyme, you...DNA Agarose Gel Electrophoresis Introduction Restriction enzymes are naturally occurring bacterial endonucleases...enzymes and the unique sites they recognize, restriction digests have become the most widely used method...cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid insert and vector...

...2 - Getting high school students and teachers interested in JEI (the importance of word of mouth and personal...required for cytokinesis in JEI. Developing an interested in biology, working in a lab for the first time...

...Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as...genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe University of Agriculture...

...and country. This information can be of personal interest to the depositor, but it can also be valuable ...annual reports. While the raw data is, of course, interesting to parse through, aggregation of this information...

...move from microbiology to law? How did you get interested in pursuing law? BN: Life science was always ... general counsel at a small start-up is very interesting. It’s very varied. There’s IP work to do, general...

...specificity allows scientists to target proteins of interest for labeling, quantification, purification, chromatin...the spleen of mice injected with the antigen of interest. The immortalized cells, called hybridomas, are...

...decision. In the open-access Data Hub, researchers interested in the Addgene antibody collection can look at... selecting an antibody! We encourage everyone interested in the Addgene antibody collection to use and...

...Even that grim estimate might prove to be an underestimate. Given all the excitement and speculation, we...ongoing outbreak from the CDC and WHO. - For anyone interested in EBOV’s past, present, and future, don’t miss...

...commercial kits you will adopt for the extraction. Restriction enzyme based approaches can require several hundreds...cloning grade DNA for direct cloning of your gene of interest into a plasmid backbone. Transfection grade...

...Citing Article" - such as cloning their gene of interest into a previously deposited vector - then you'...locate other papers that have used your plasmid of interest, which may help you identify relevant protocols...

...trainees at all. For grad students and postdocs interested in taking matters into their own hands, there...I am happy to work with any group of trainees interested in designing career exploration programs or peer...

... from your sequencing runs.   When you run a restriction digest on a gel you always include proper controls...primer at least 50bp upstream of the sequence of interest. 5. Use a silica spin column for purification ...

...this plasmid has a U6 promoter followed by BbsI restriction sites into which you can clone your 20 nucleotide...can use to express both SpCas9 and your gRNA of interest in a variety of mammalian cell lines. Alternative...

...to be used as research tools is particularly interesting. Although humans have known about bioluminescent...deciding which one is right for you, so if you’re interested in learning more about fluorescent proteins, ...

...Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The most requested...next Top 10 list. 10 years of plasmid sharing  Interested in more facts about Addgene's 10 years of plasmid...

...can be applied non-invasively, its use is not restricted to cultured cells but extends to freely moving...plasmids have clear application to anyone with an interest in neuroscience, their use is by no means limited...

...discussed further in this informative post. Interestingly, this technology may make some uses of Site ... Software Engineer Kris Shamloo wrote a very interesting post about how we develop our in house lab management...

...screen for pathways that confer any phenotype of interest, not just drug resistance,” he said. “It could...contacted by investigators all over the world who are interested to use our libraries to do things - sometimes...

...characteristics and ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific...