We narrowed to 749 results for: rev

...detecting genome-wide double strand breaks (BLESS), revealed that these mutants do not cause off-target effects...to their enhanced specificity. Additional work revealed that mutations in the mobile Rec3 domain facilitate...

...melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A....number with links to the plasmid pages for your review. We’ll also mail you a deposit kit with instructions...

...peptide, the CRY2clust domain enables fast and reversible oligomerization when fused to a protein of interest...within seconds after a pulse of blue light, and is reversible upon termination of light stimulation. CRY2clust...

...to light as antibodies became commercialized, revealing the unreliability of antibodies. There are three...reproduced and projects that cannot be completed. A 2015 review analyzing and explaining the antibody crisis states...

...about choosing AAV or lentiviral vectors in our previous blog posts.  Viral vector injection Viral vectors...be involved in a certain type of behavior (for a review see Stoica et al., 2013). Vector delivery may also...

...otherwise be lost in lab notebooks forever. microPublications are peer-reviewed and assigned a DOI that can ...

...enter (and thus infect) cells, this safety feature prevents the virus from replicating and spreading in non-target...Types of Viruses Viral Vector Elements Tips for Preventing Viral Recombination  ...

...Step 3: Reviewing the plasmid summary on the Fragmid website.   Step 3 is simply a review of the ...

...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can.... Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases on ...

...based methods for viral titration consider using a reverse transcriptase mutant in a separate control reaction...over-inflate results. A packing vector deficient in reverse transcriptase will produce low levels of virus ...

... histones, the more compacted it becomes. This prevents the cellular machinery from physically interacting... further reading and viewing The Epigenetics Revolution: How Modern Biology is Rewriting Our Understanding...

...need to be signed, or manuscripts that need to be reviewed, bring them to the meeting. This will make it ...items. This will give your supervisor a chance to review what you have discussed and add suggestions if ...

...to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture...proteins at endogenous loci, shedding light on previously unknown protein functions. References Schwartz...

...from 4) - Dunn et al. 2011 provides an in-depth review of quantitative colocalization analysis methods...wavelengths, respectively, are selected (6). These previous blog posts help with the decision of which FP ...

...Trends in Pharmacological Sciences, a Cell Press reviews journal. Stem cells are special types of cells...Trends in Pharmacological Sciences, a Cell Press reviews journal. She joined Cell Press to pursue a career...

...insert), mostly due to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The... less. The list goes on... In this post, we’ve reviewed many of the most popular cloning methods out there...

...before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should...

... yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used species... in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

...to, like ones that are hidden in the molecular crevices proteins on the cell surface. Figure 1: ...the enriched pool of sybody candidates are then reverse transcribed into cDNA fragments that are efficiently...

...by encapsulating a single enzyme. Compared with previous research, which required protein compartments ...inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression...