We narrowed to 538 results for: sequence

1. Antibodies 101: Introduction to Antibodies

   Type

   Blog Post

   ...searching for. Antibodies may recognize a linearized sequence of amino acids, or only in their 3D conformation...recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity and sensitivity...

2. Hot Plasmids - October 2022

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   Blog Post

   ...saturating mutagenesis on 21 positions in the protein sequence of the predecessor GEVIs (called ASAP 1 and 2)...

3. Addgene Begins Distribution of Recombinant Antibodies

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   Blog Post

   ...community-building. The transparency begins with sharing sequence information for the antibodies in our catalog,...

4. Plasmids 101: Gene, enhancer, and promoter trapping

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   Blog Post

   ...Unifying the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org...

5. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   Blog Post

   ..., recombination sites. These sites are target sequences of PhiC31. In the SPARC system, attP sites are...

6. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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   Blog Post

   ...the names imply, PAM-out designs have the PAM sequences on the extremes of the targeted region, whereas...

7. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   Blog Post

   ...resolution imaging. mNeonGreen also has little sequence identity to other common fluorescent proteins ...

8. Understanding GPCR Signaling Using TRUPATH

   Type

   Blog Post

   ...protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016...

9. Some Like it Hot: Thermostable GeoCas9

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   Blog Post

   ...SpCas9 GeoCas9 Size 1368 AA 1087 AA PAM Sequence NGG CRAA (R = A or G) Spacer Length 20 nt...

10. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...the molecule directly into the protein’s peptide sequence. Thus, all synthesized target protein molecules...until they bind a 6-residue tetracysteine tag (sequence: CCPGCC) that can be inserted into the target ...

11. Adenovirus Plasmids

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    Collection

    ...recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule can then...

12. Antibodies 101: Epitope Tags

    Type

    Blog Post

    ...cells as Caspase3/7 both cleave after the DVPD sequence. Check out Addgene’s monoclonal antibodies (mouse...

13. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    ...complete spectral information, accession numbers, sequences and lineages, FRET efficiencies, additional references...

14. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post

    ...which binds to a small (< 20 amino acid) AviTag sequence in a protein and adds biotin to a lysine residue...

15. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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    Blog Post

    ...description, how the plasmid was generated, validated sequence information, and more. The plasmid page also helps...

16. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    ...and only the fluorescent protein and 3xFLAG-tag sequences are inserted into the genome. Figure 1:...

17. Advice for Scientists Starting a Lab

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    Blog Post

    ...interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results to companies.” Take...

18. Antibody Plasmid Collection

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    Collection

    ...ability to optimize the antibody’s antigen binding sequence to improve binding and reproducibility. Some scientists...

19. Zinc Finger Consortium: Zinc Finger Arrays

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    Collection

    ...vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression...

20. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post

    ...anchoring domains – GPI and SGZ. Fig: 4 Sequence schematics for PDGFR, GPI, and SGZ display constructs...