...responsibility, repeated reminders of blunders, setting target up to fail, shifting goal posts without ...has no idea who is dating whom Expanding your skillset Scientists are lifelong learners. I believe we...

...use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired immune system in bacteria...Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing...

...replicated easily and efficiently in a laboratory setting. Stable - Plasmids are stable long-term either ... in many species and can be useful for a diverse set of applications - Plasmids can drive gene expression...Genetic Code The genetic code can be defined as a set of rules for translating the information encoded ...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...

... discovery of RNA guided transposons in bacteria sets the stage for combining the super powers of CRISPR... transposons encode reporter genes, mutagenic cassettes, or barcodes. When transposons are delivered to...

... done things that I never even imagined before I set foot in the lab. They’re also more than eager to ...

...different-sized multimers, you may even see different sets of bands on the gel corresponding to each type of...

...its expression, and is flanked (“floxed”) by two sets of incompatible recognition sites (e.g., loxP and...

...initiates different immune cascades. In the research setting, antibodies of different isotypes can be used together...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

...is orthogonal to their scientific research skill set. You'll need to motivate your trainees, communicate...

...solidified. Pro-Tip If you are in a hurry, the gel will set more quickly if you place the gel tray at 4 °C earlier...increases the density of your DNA sample causing it settle to the bottom of the gel well, instead of diffusing...

...tube for 5 min at room temperature on the highest setting. Note: You should see clearly separated layers:...

...anise?” This is something I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted...

... easier to automate and use in high-throughput settings, but when it comes to low-throughput applications...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...reactions with relatively little work after the initial setup. (Addgene actually uses some of these — if you’re...

... Mello labs also identified several experimental set ups that could improve CRISPR efficiency in C. elegans...introduced. Cre-mediated excision of the selective cassette minimizes the sequence added, leaving a 34 bp ..., however the development of the self-excising cassette (Dickinson et al., 2015) made this step as simple...Engineering with a Self-Excising Drug Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534...genetics.120.303564 Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-CRISPR...

...March for Science in Albany, NY, many tables were set up with various science demonstrations, and students...

...microscopist at your institution when heading into a new set of experiments. Many thanks to our guest blogger...number of optical and chemical ‘tricks’ to turn subsets of fluorescent molecules on and off. This can provide...

...addresses these questions, which are critical in setting up a FRET-based assay or screen (the Addgene blog...

...optimize your activation protocol for your particular set up. Applications of photoactivatable fluorescent ...and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast...

...Gersbach Lab multiplexing plasmids: This plasmid set allows you to express 2-4 gRNAs, with four being ...including Standard PolIII-gRNA cassette, Csy4-cleavable cassette, and PTG cassette.    Find gRNA multiplexing...smaller than constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors...aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" ...