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We narrowed to 846 results for: tat

Showing: 401 - 420 of 846 results

  1. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ..., allows us to display high-quality maps and annotations without us having to invest too much of our internal...partner, connect the relevant teams, coordinate implementation, and now must focus on managing the established...I personally felt that I had to overcome some hesitation to ‘approach strangers’ and learn how to communicate...maintaining useful notes that remind me about the current state of a partnership. Every collaboration and partnership...

  2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...These off-target sequences may be unintentionally mutated along with the desired target locus. The PAM sequence..., we will cover three ways to circumvent this limitation: 1) the use of novel S. pyogenes Cas9 variants...PAM binding specificity: SpCas9 variant Mutations (relative to SpCas9) PAM sequence D1135E...human genome. Another variant, xCas9 3.7 has 7 mutations found in the REC2, REC3, and PAM interacting domains...

  3. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...determine the presence and quantity of DNA, RNA or a mutation of interest. Together SHERLOCK and DETECTR demonstrate... specificity.  SHERLOCKv2 SHERLOCK had a few limitations when it was first introduced in 2017 so the lab... pre-amplification step allowing for greater quantitation without compromising sensitivity. To detect...SHERLOCK could also detect low-frequency cancer mutations from cell-free DNA fragments as well as health-related...

  4. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...have since been initiated and today thousands of mutations in genes affecting diverse biological processes—not...deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish using zinc-...depending on whether a gene is deactivated via mutation or morpholino. This observation unlocked novel... been tracked using microscopy and advanced computational approaches (Keller et al., 2008). This microscopy-based...

  5. Bioinformatics at Addgene

    Type
    Blog Post
    ...data pipeline is to perform several sequential computational tasks for which existing script(s) have been...  We started at the usual recommended place: documentation. We oriented ourselves with the basic concepts... the file name. We started small with a print statement and tested that the desired data was available...we manually inspected the output with a print statement at the end of the script or by opening the files...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated pellets...i.e., genome-containing) AAV particles within representative vector preparations was quantified with electron...for viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids...

  7. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ... same (forward) or in the opposite (reverse) orientation? The answers to questions like these will determine...sequences, called modules or parts. Assembly is dictated by four-base overhangs that are paired with different... the sequence, normally through the synonymous mutation of a codon. You can domesticate the sites yourself...colony PCR electrophoresis), is free of undesired mutations, and that you have the correct four-base overhangs...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...TBD domains, part of the HB domain, and several mutations to improve solubility and stability (Figure 2)...through eight stages of embryonic development. Representative images of single focal planes through the middle...in natural DNA repair pathways and spontaneous mutations.  Here, the researchers were able to target multiple...abscessus either simultaneously or stepwise, achieving mutation efficiencies of up to about 9% of colonies (102...

  9. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ... constant variables that enable the correct interpretation of the effect of the independent variable you...controlled for in order to eliminate any alternative interpretation of the results. Typically, plasmid-based experiments...non-targeting control is critical to the correct interpretation of the results because it provides an important...etc, but outside constraints may impose some limitations and you should be cognizant of these when interpreting...

  10. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...sense OR antisense transcript depending on its orientation to your gene. T7lac High levels of gene expression...sense OR antisense transcript depending on its orientation to your gene. araBAD General expression Promoter... promoter with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus...sense OR antisense transcript depending on its orientation to your gene Although this list is a great...

  11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-CoV-2 has... fluorescence can be observed under either no excitation light, UV light or LED blue light.  Highlights...to our guest bloggers Shravanti Surest from Iowa State University. Shravanti Suresh is a PhD candidate ...CRISPR/Cas immune systems in the Sashital Lab, Iowa State University. She is actively interested in science...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...glutaraldehyde crosslinking: representative micrograph (C), particle orientation distribution and Fourier-...natively isolated complexes. To address these limitations, the Funabiki Lab developed a technique called...of LEAs may bias the distribution of particle orientations, this could be overcome with methods such as...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...recombination target sequences, denoted by triangles] facilitate tissue or cell type specificity. An on/off genetic...administered intravenously. To address these limitations, labs have developed techniques like CREATE (...broad):  enhanced coverage for inhibitory versus excitatory neurons Mice, rats Yes (LY6A) Chan et al...AAV.CAP-B10 CNS: balanced coverage of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et... its tropism is relatively more biased toward excitatory neurons than AAV-PHP.eB (Fig. 4c, Jang et al....administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque ...

  14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...first generate your desired mutation in mouse ES cells, select for the mutation and inject the ES cells into... models. For simple knockout alleles or point mutations, the 8-10 month process of conventional gene targeting...PAM; if not, you can add a silent PAM or guide mutation. Designing an Easi-CRISPR conditional allele The...

  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...hearing about researchers' favorite features and limitations of existing backbones, we're excited to share...5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts the identification...gene promoter using TurboCas, streptavidin immunoprecipitation (IP), and tandem mass tag (TMT) mass spectrometry...interneurons — those marked by parvalbumin (PV), somatostatin (SST), vasoactive intestinal peptide (VIP), ...

  16. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...which can be switched from a default OFF to an ON state, and how you might use these in your research. Today...which can be switched from a default ON to an OFF state, as well as repressible binary systems. In recent...domain VP16. cTA binds to promoters containing putative operator sequences (CuO) (linked in groups of ...QUAS promoter is less leaky than UAS in the basal state, and co-expression of the inducer QF can increase...

  17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...nucleotide sequences with high specificity and quantitative accuracy and even include profiling single nucleotide...Electrochemiluminescent assay for detection of extremely rare mutations based on ligase reaction and bead enrichment."...direct identification of allelic composition of mutated DNA in a mixed population of excess wild‐type DNA...probe for the detection of low-abundance point mutations." Analytical chemistry 86.1 (2013): 900-906. PubMed...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...of the population as a whole, scientists gain statistical power in their observations. However, while flow...Techniques is Best for Me?  An overview of the instrumentation A flow cytometer combines three systems to ...fusions by binding to the protein in its native state.  Antibodies-based flow cytometry can use both direct...cytometry: retrospective, fundamentals and recent instrumentation. Cytotechnology 64:109–130 . https://doi.org...

  20. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ... V2.0 from it's predecessor, which had a point mutation in the puromycin resistance gene that made it ...less effective in certain cell lines. This point mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro...than creating double stand breaks and may better facilitate homology directed repair. You can find all of...
Showing: 401 - 420 of 846 results