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We narrowed to 800 results for: tec

Showing: 401 - 420 of 800 results

  1. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...features with gamma-retrovirus including genome architecture, host integration, and its association with ...Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713...An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390...

  2. How Addgene Creates Content

    Type
    Blog Post
    ...gaps and add to them as techniques evolve. Blog posts get out of date as techniques and knowledge changes... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...

  4. Antibodies 101: Isotypes

    Type
    Blog Post
    ... IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly... using monoclonal antibodies Read about other techniques that you can use antibodies for in our Intro ...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...heterotrimeric G protein complex that can be used to detect G protein activation via bioluminescence resonance...response in vivo (Paek et al., 2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation...are then identified with mass spec. Using this technique termed “GPCR-APEX” the Kruse lab was able track...

  6. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...PI labs and institutions. There are many more technical details that make our new search engine unique...

  7. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ... creativity in employing a variety of cloning techniques and troubleshooting methodologies drew me to ...antibodies are commonly used in research laboratories to detect the different subclasses, but there are also secondary...use of subclass specific secondary antibodies to detect multiple targets with different mAbs in the same...

  8. Antibodies 101: ChIP

    Type
    Blog Post
    ...biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques used, but...immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions...

  9. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...excitation by a laser, which is then read by a detector panel in the machine. Fluorophores are light sensitive...different signaling molecule, that all need to be detected in the same assay. This is one of the most complex...signal "spilling over" into another channel's detection range. Though you can compensate for this when...

  10. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness is an important...brightness that does not fold well or cannot be detected in a microscopy setup has zero practical brightness...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...

  11. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated... is one of the latest developments in CRISPR technologies.  It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...

  12. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ... require access to next generation sequencing technology. These libraries are lentiviral in nature, ...

  13. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...to essentially any DNA sequence desired. This technology allows you to create a pair of designer proteins...on traditional homologous recombination. That technology had been used for decades to generate knock-out...That’s the revolutionary aspect: TALENs take a technology that was difficult for all but the most experienced...

  15. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...experiments that require multiple antibodies that each detect a different antigen. To pre-adsorb the antibodies...section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational...Overlooked benefits of using polyclonal antibodies. BioTechniques 65:127–136. https://doi.org/10.2144/btn-2018...

  16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural activity using a red fluorescent genetically...encoded voltage indicators (GEVIs) are proteins that detect and output the voltage changes in neural activity..., has increasingly been recognized for its biotechnological applications including conversion of CO2 into...

  17. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... an antibody that is directly conjugated to a detection substrate, such as a fluorophore, or a conjugated...do you know about the vendor and the vendor’s technical support? An antibody from a vendor that you use...frequently, with a responsive and knowledgeable technical support team, is a safer bet than one from a large...

  18. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...it, his lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical... collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making ...all heading? Voigt: The top products out of biotechnology today are things like butanol - very simple ...

  19. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...powerful development in neural tracing. Using these technologies in combination with molecular tools (e.g., optogenetic...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...described above, and not any particular cloning technique. Though rarer, you can find MoClo that relies ...assembled into many combinations. Even with current technologies, it can be extremely hard to predict how a single... if you want to learn more about this cloning technique! Many thanks to Rob Hurt for his help on this ...
Showing: 401 - 420 of 800 results