We narrowed to 794 results for: tec

...excitation by a laser, which is then read by a detector panel in the machine. Fluorophores are light sensitive...different signaling molecule, that all need to be detected in the same assay. This is one of the most complex...signal "spilling over" into another channel's detection range. Though you can compensate for this when...

... IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly... using monoclonal antibodies Read about other techniques that you can use antibodies for in our Intro ...

...heterotrimeric G protein complex that can be used to detect G protein activation via bioluminescence resonance...response in vivo (Paek et al., 2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation...are then identified with mass spec. Using this technique termed “GPCR-APEX” the Kruse lab was able track...

...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...PI labs and institutions. There are many more technical details that make our new search engine unique...

...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ... require access to next generation sequencing technology. These libraries are lentiviral in nature, ...

...to essentially any DNA sequence desired. This technology allows you to create a pair of designer proteins...on traditional homologous recombination. That technology had been used for decades to generate knock-out...That’s the revolutionary aspect: TALENs take a technology that was difficult for all but the most experienced...

...Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural activity using a red fluorescent genetically...encoded voltage indicators (GEVIs) are proteins that detect and output the voltage changes in neural activity..., has increasingly been recognized for its biotechnological applications including conversion of CO2 into...

... by the reliance on pH-based biosensors used to detect the maturation of neutral vesicles to more acidic...For further reading on fluorescent microscopy techniques, the first author of this article, Dr. Douglas...with classical gene overexpression and knockout techniques. Find the plasmids from Aranda-Díaz et al. Aranda-Díaz...

... an antibody that is directly conjugated to a detection substrate, such as a fluorophore, or a conjugated...do you know about the vendor and the vendor’s technical support? An antibody from a vendor that you use...frequently, with a responsive and knowledgeable technical support team, is a safer bet than one from a large...

...experiments that require multiple antibodies that each detect a different antigen. To pre-adsorb the antibodies...section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational...Overlooked benefits of using polyclonal antibodies. BioTechniques 65:127–136. https://doi.org/10.2144/btn-2018...

...it, his lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical... collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making ...all heading? Voigt: The top products out of biotechnology today are things like butanol - very simple ...

...updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins...be engineered to “armor” therapeutic phages to protect them from pathogenic bacteria’s CRISPR-Cas system... applications: natural brakes for CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038...

... school when she was hired full time as a Lab Technician. “I was so tired of being in school so I just... is not true at all. Racheal Komuhendo, a Lab Technician, dreamed of becoming a doctor. After taking a...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...

...described above, and not any particular cloning technique. Though rarer, you can find MoClo that relies ...assembled into many combinations. Even with current technologies, it can be extremely hard to predict how a single... if you want to learn more about this cloning technique! Many thanks to Rob Hurt for his help on this ...

...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field... obstacles for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges...taken advantage of what our current delivery technologies are suited for. For example, lipid nanoparticle...

...antibodies and found that across suppliers there was a detection bias of IgG2a > IgG2b > IgG1 (Manning 2012).  ... the anti-mouse secondary antibodies used for detection bind to non-target immunoglobulins naturally present.... Chapter 15 Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods...

...of our mouse modeling blog series, we covered techniques that can be used to introduce genetic modifications...conditional knockin or knockout mice. While these techniques are each useful for introducing one modification...) and using PCR with the appropriate primers to detect your targeting vector or gene of interest. 2. Breed...

...very easy — our tech transfer team will communicate with your organization’s technology transfer office... ship the samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download...

... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...powerful development in neural tracing. Using these technologies in combination with molecular tools (e.g., optogenetic...

...biological tasks, both of which are factors in the technical challenges they can present. Many researchers ...hail Marys that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors... quantification as measured with branched-DNA technology." Journal of clinical microbiology 37.6 (1999...