...meetings as opposed to phone calls most closely replicates how you’d interact with team members in person...helps everyone feel included in the project and accelerates the pace at which problems can be addressed and...the times that work best for them. This also accelerates the pace of research by circumventing the slower...techniques are being performed the same way at different sites. I did a lab exchange as part of the collaboration...

...complex, a model may be most effective. Once drug candidates are isolated, they can be validated for specificity...even be used to generate patient mutations and then test both individual and combinatorial drugs on for effectiveness...and validate the hit compound. The standard is to test the drug on models which are genetically proficient... can save time and money to make sure quality candidates are selected.      CRISPR aids in multiple...

... at eScience Labs, an educational company that creates hands-on science lab kits and digital curricula..., how to design good lesson plans, how to design test questions, and how to design lectures. I really ...happenstance. It was on Indeed or one of the job search sites. It's  a rare trek these days to go in cold turkey...

...often better. More transcription factor binding sites in a promoter lead to higher transcriptional activation...This workaround risks cellular phototoxicity and eliminates the possibility of long-term imaging studies....Overexpressed proteins also have the potential to form aggregates and may be toxic to the cell. Here comes the ...kinesin across microtubules. Tanenbaum et al. then tested their hypothesis that the lower expression levels...

...recognized and operated as a nonprofit under the United States Internal Revenue Code 501(c)(3). That means we ...project we undertake and every service we provide is tested against fulfilling our mission. “Addgene’s nonprofit... post. We feel GREAT when you send us thank you notes and recognition. You are why we do what we do. ...Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

...Addgene is thrilled to announce the launch of our latest community resource: Recombinant Antibodies! In ...which the recombinant antibodies are derived were tested for specificity in knock-out lines or animals.  ...include information about immunofluorescence, conjugates, isotypes, and more. How You Can Participate ... represent the official views of the National Institutes of Health....

....  To develop HA frankenbody, the Stasevich lab tested several scFvs scaffolds previously proven to fold...frankenbody is that it remains bound to HA epitopes for minutes in living cells, meaning that scientists can track..., D. (1972). Localization of antibody-combining sites within the variable portions of heavy and light ... the National Academy of Sciences of the United States of America, 69(9), 2659–2662. PubMed PMID:4560694...

...(pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium... for imaging of small neuronal processes like dendrites and axons. Find the 7b variant of jGCaMP7 in AAV1...is the primary inhibitory neurotransmitter in vertebrates, but methods for directly imaging GABA have been...pAAV.hSyn-FLEX.iGABASnFR.F102G) is noticeably dimmer than iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger...

...was locked down, a South African company wanted to test if a type of antibodies from an Andean mammal called...preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite the...volunteers are extremely diverse. Some of them are undergraduates or PhD students who are just starting their...trial-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations are...

...during Zoom lab meetings to chip in ideas and take minutes that can be accessed by anyone at any time. This... be written by several authors from different institutes, disciplines, and cultural backgrounds.” This...simply moves away from the desk to switch off a few minutes. The host could also set aside Breakout Rooms to... allowed with a different host each time that moderates conversations. This can help team building and...

...sessions. “At big conferences, you could walk 20 minutes between one session to another,” Bachle says. “...note-taking doc where participants could share their notes together. Figure 1: Can you draw Spongebob...and making sure you stay connected. If possible, test out the technology before the conference begins....after the meeting, I received a lot of LinkedIn invites.”Bachle pointed out that networking at virtual ...

...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds,...can be counteracted by growing the cells on solid plates, where each transformant forms its own colony, ...solid media. The colonies are then scraped off the plates and pelleted for DNA extraction. Prepare library...using both pre- and post-amplification DNA and generates PCR sequencing products from both. These products...

...dissociation rates and discovered that the catalytically active form of the nuclease dissociates very slowly...our surprise, we measured identical dissociation rates for catalytically inactive dCas9 molecules. We followed...precisely spaced region allowed sequence replacement rates of approximately 1%. This is by no means a large...

...is a challenge. To help keep you abreast of the latest fluorescent proteins, I maintain an interactive...match to the filter set but the excitation filter excites more of the peak of the TagRFP excitation and the...to be extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent proteins ...optimal for all organisms and that if you want the brightest signal, you may need to try several proteins in...

...of Producing Monoclonal Antibodies, 1999). This creates an immortal cell population that will continue ...of molecules including proteins, lipids, and carbohydrates, in this blog post we’ll focus on antibodies...lots over time. Specifically, the antigen-binding sites of the antibody - the paratopes - may change and... Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody project ...

...control AAV in smaller aliquots of 20ul for serotype testing to help you pick the best AAV for your experiment...GABA-ergic interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid...activated, this DREADD acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor...functional Cre enzyme that induces recombination of lox sites. This system takes advantage of the extensive supply...

...none of these constructs have been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids...combinations of WT, codon optimized, untagged, cleavage sites (thrombin and TEV), and C-terminal tags (StrepII...first pass QC. The lab team manually transforms, innoculates, and performs DNA minipreps for small batches...mutation.  Once the samples arrive, each group of plates will have select samples prepared and screened ...

... PAM specificity, allowing for the targeting of sites that were previously unavailable. Figure ... with a broader PAM specificity can target more sites. Created with BioRender.com. Specificity Specificity...variants that were able to target different PAM sites (NGG, NGAN, NGNG, NGAG, and NGCG). Since then, the...eSpCas9 and SpCas9-HF across a number of different sites. Using a combined random and directed mutagenesis...

...cleavage, and suppresses neuronal transmission within minutes. Constructs using the photodimerizer system CRY2...Escherichia coli and Salmonella Typhimurium. This work demonstrates engineered gas vesicles as the first reporter...is regulated in the 3’ UTR by four miRNA target sites and thereby serves as a sensor of miRNA presence...population, or identify differentiation and disease states. This collection of reporter plasmids is amenable...

... 49,000 times! Their long-lasting popularity illustrates the utility these tools have in many different...pyogenes with an invariant sgRNA scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). ...notice the “V2.0” at the end of this plasmid which indicates that there was a prior version of this plasmid...retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev...