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We narrowed to 653 results for: EPO

Showing: 211 - 220 of 653 results

  1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...Biotechnologies' press release about their Cas13d deposit here). Konermann et al.: CasRx is a highly potent...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...continue to grow, and we at Addgene appreciate our depositors for making their tools available to the broader...

  2. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoter, thereby activating transcription. Addgene depositors have developed heat shock-inducible Cre and Cas9... phage promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, ...preventing repressor cI synthesis and allowing reporter gene transcription to take place. Addgene also...

  3. How Addgene Creates Content

    Type
    Blog Post
    ...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...informative resources that answer questions our depositors and customers are asking. So how can you get ...

  4. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...

  5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...pH-sensitive fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system...2008). Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor. Scientific Reports,5(1). doi:10.1038/srep12397. PubMed PMID: 26215030...

  6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...do? Let’s take a look at how plasmids recently deposited by Chris Voigt’s lab help keep gene expression...buffered against the effects of copy number on reporter gene expression. On the other hand, using a constitutive...Download the Plasmid 101 eBook See all plasmids deposited by the Voigt lab ...

  7. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...by reducing the distance between the target and reporter. They have fewer non-specific binding opportunities...it either needs to be directly conjugated to a reporter or you need a light-chain specific conjugated ...fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make good secondaries...

  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...community. This is no small task, however, at a repository that consistently receives around 200 new DNA...data on every plasmid we distribute through our deposit process, and then we carefully track samples through...your effort. In addition, if you should choose to deposit your plasmids with us, it will be easy to find ...

  9. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research investigator in the...to EGFR inhibitor (Hata et al., 2016).  Bhang deposited the library with Addgene because she wanted to...sharing protocols one by one,” and she is excited to deposit the library with Addgene because it is a “really...
Showing: 211 - 220 of 653 results