...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns...

...localization, dynamics, and behavior of a POI. As an investigator, you want a fusion tag to be inert, to not produce...has gone into making FPs monomeric, yet many investigators remain ignorant of FP dimerization. Equally ...Einstein College of Medicine in the Department of Anatomy and Structural Biology. His interests include the...

...expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate...  The first successful attempt to render fixed anatomical preparations transparent was done by Walter Spalteholtz...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

...genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer ...fluorescent intensity upon pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next ...

...you possess because any potential employers, collaborators, or co-workers want to know that you are a well-rounded...situation? Maybe you demonstrated empathy for a collaborator or co-worker and showed that you really understood...Resources Cross team communication  External collaborators For further advice on highlighting your transferable...

...communication should be fun and engaging for the communicator as well as the audience, so pick a mode of communication...Otherwise, you’re going to drop your project like a hot potato or burn yourself out trying to slog through unappealing... Low barrier to entry for both audience and communicators. Well-developed content creation platforms.  ...

...and debris. Affinity chromatography is a popular choice for this. Chromatography columns containing appropriate...using a desalting column or an ultrafiltration concentrator. As the name suggests, you may also concentrate...

...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...production of the Cas9 protein and a gRNA in your laboratory, once these have been acquired, genomic editing...

...pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing, aka determining where...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be...

...do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to...precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional activation from the 5’ ...trials for other diseases, including the common hematopoietic disorders sickle cell anemia and beta-thalassemia...