We narrowed to 728 results for: STO

1. New Tools Enable CRISPRa for Neuroscience Applications

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   ... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS.  Thus, the ...

2. Lab to Office Culture Shock

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   ...cities around the world, such as BosLab here in Boston. Organizations like this one are always looking...

3. CRISPR-mediated Plant Base Editors

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   ... this mutation by an adenine base editor would restore antibiotic resistance. To find such a protein, ...

4. Overcoming the AAV Size Limitation for CRISPR Delivery

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   ...post-injection. Livers from these mice were histologically normal and liver injury markers were not increased...

5. Hot Plasmids - August 2020

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   ... localization signal or including fusions with Histone 2A or 2B can result in bright targeted expression...

6. Antibodies 101: Immunoprecipitation

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   ...technique to identify DNA binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used...

7. Transferable Skills Guide: External Collaborations

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   ...keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I just had...

8. QC Sequencing Technologies at Addgene

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   ...ddNTP) so that after its addition, DNA synthesis stops. The resulting DNA fragments are pooled and then...

9. Plasmids 101: Shuttle Vectors

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   ...sequences are translated into amino acids. And I was astonished that the molecular language shared by every living...

10. How to Be an Excellent Trainee

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    ... go ahead and write down where that one drug is stored or how to access the shared server. The blue box...

11. Viral Vectors 101: Preparing Pooled Libraries

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    ...want to) make viral preps in your lab, there are custom viral prep services that can prepare the libraries...

12. Using Video to Share Your Science: We Share Science

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    ...is not hard either. Here are some resources: ImpactStory, a website that helps researchers go beyond citation...

13. Changing Labor Laws Bring Increased Postdoc Wages

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    ... all already have salaries at or above $50,000. Boston Children’s Hospital is the only part of the Harvard...

14. CRISPR Activation: A Practical Guide

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    ...plastic tool and has taken the scientific world by storm. While Cas9 has been most widely used to create ...

15. Tips for Writing a Good Cover Letter

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    ...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...

16. Plasmids 101: Broad Host Range Plasmids

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    ...properties that influence host range are not fully understood, but scientists have identified some of the features...

17. Plasmids 101: Gateway Cloning

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    ...elements (ribosome recognition sequences, start codon, stop codons, reading frame considerations, etc). This...

18. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...presence of these two factors are not completely understood, the addition of an shRNA for P53 and replacing...

19. Important Considerations in Optogenetics Behavioral Experiments

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    ...reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting an electrode and...

20. Plasmids 101: E. coli Strains for Protein Expression

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    ...purification protocols are designed for soluble, cystosolic protein products, but this is not always achievable...

21. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins...

22. Harnessing Bacterial Toxins for Allelic Exchange

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    ...postdoctoral researcher at Harvard. There’s an astounding number of ways to create chromosomal mutations...

23. Hot Biosensors 2022: Year-End Roundup

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    ...10.1021/acssensors.1c02201. LiLac sensor for lactate Stop and smell the flowers - or rather, the lactate! ...

24. Antibodies 101: Introduction to Immunofluorescence

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    ...with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these terms refer to...

25. Adeno-associated Viruses (AAVs) for Genome Editing

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    ...Although the mechanism of incorporation is not well understood, researchers have been using AAV vectors for ...

26. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G), which facilitates viral...

27. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...GTPase optogenetic systems (Berlew et al., 2022). Stopping traffic with CIB1-Rabs Instead of using an optogenetic...

28. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...CRISPR has taken the genome engineering world by storm owing to its ease of use and utility in a wide variety...

29. CRISPR 101: Homology Directed Repair

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    ...single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017.  Easi-CRISPR...

30. Transferable Skills Guide: Time Management

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    ...final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly to see ...