We narrowed to 931 results for: TIM

...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

...changes simultaneously with 54% efficiency, about 7 times higher than ABE7.10. However, the increased editing...the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution of naturally ...

...acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes can...meaning that there will be changes between lots over time. Specifically, the antigen-binding sites of the ...

...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog...

... of Cas9 expression between experiments and over time (as compared to separate transductions with a single-vector...(Gi)-mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression...

... folder that you are currently working in) every time you use them, leave them in their own folder. Doing...Organized Learn about Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software...

...have some homology to each other; ~17 bases is optimal. After being treated with DpnI to digest away the...-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works in your...

...process: It is important to give your plates enough time for any intact β-galactosidase to be expressed and...is evenly distributed and allow sufficient drying time before use. Beware of false positives: Blue-white...

...In the short time since its development, CRISPR/Cas9 genome editing has been used to study the effect...transcript levels of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et...

...a recent plasmid deposit please sign up here. No time to read? Listen to our Hot Plasmids Podcast! TRIM-Away...modules to repress or activate gene expression, sometimes incorporating chemically inducible approaches ...

...only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially stained with two HRP...expensive Can only stain with one HRP-RANbody at a time GFP, RFP, H2A2B, Gelsolin Spaghetti Monster: ...

...degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part...4.9 - 9 SEP 488* nm 508 nm ~6.5 - 9 *Optimal sequential excitations wavelengths as determined...

...has thus benefitted from countless rounds of optimization. Dozens of variants exist with unique cutting...and diversify PAM sequences of Cas12a to further optimize it for therapeutic and laboratory usage. Fast...

...the activity or function of a single protein? Oftentimes, they rely on protein purification. In this strategy...to the metal ions once the protein is folded. Sometimes a tag placed on one end will be blocked during...

...have been harnessing the power of dsRNA for a long time, even before we knew about all the diverse in vitro...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...

...isolating total RNA, this method requires a lot of time and depending on how many samples you may have, ...isopropanol precipitated. This method reduced the time it took to isolate RNA from 20+ hours to around ...

...advantage of this alternative is the possibility of real-time imaging. Robert Singer’s lab at Albert Einstein ...tag after pulse labeling of a determined period of time. Thus, only newly synthesized proteins of interest...

...the threshold at which salaried workers receive overtime payment for working more than 40 hours per week...following feedback from postdocs and faculty (at time of writing, Brigham And Women's in Boston also just...

...“Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology by integrating... one thing, cells and cell lines degenerate over time—and sex can be hard to determine. To remedy this...

...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

... workhorse protein even more useful! From FPs optimized for oxidizing environments to photoconvertible...sophisticated experimental manipulations, saving the time and money needed to develop new lines. Using nanobodies...

...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. Selection...expressed only in specified cells or at specified times. Combining this with some of the loxP methods described...

...be required by one or more NRs for efficient and timely activation or repression of gene expression (see...the research literature and acquiring them in a timely manner. Addgene represents an excellent solution...

...for a year, I’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a...courses in social, health or policy-related topics, as time permits. Also seek out opportunities to communicate...

... a catalytically inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus...recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use lentiviral...

...not only highlighted new tools but also included time for questions and a group discussion on the best... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...interview is complete. Remember, your interviewers took time out of their day to talk with you, so be engaged...

...for targeting thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA... the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective in reducing...

...interaction is typically irreversible and requires some time for chromophore maturation, splitFAST displays rapid...Remember that the FP fragments themselves can sometimes interact in cells and produce background signal...

...Feinberg and Bert Vogelstein showed, for the first time, there were unusual patterns of DNA methylation ...undetectable amounts: ergo, DNA demethylation! With optimization, this method can produce nearly 100% demethylation...