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  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...Will it be sensitive enough? No one wants to spend time screening hits that aren’t “real” or novel for their... understand the difference between the two, it’s time to check out our CRISPR library resources or our...

  2. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...software tools for the right people at the right time. To explain a bit further, a software product manager...going to need to be a problem-solver. A lot of the time, the problem to solve is clear, but the solution...

  3. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... folder that you are currently working in) every time you use them, leave them in their own folder. Doing...Organized Learn about Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software...

  5. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...process: It is important to give your plates enough time for any intact β-galactosidase to be expressed and...is evenly distributed and allow sufficient drying time before use. Beware of false positives: Blue-white...

  6. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...In the short time since its development, CRISPR/Cas9 genome editing has been used to study the effect...transcript levels of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et...

  8. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially stained with two HRP...expensive Can only stain with one HRP-RANbody at a time GFP, RFP, H2A2B, Gelsolin Spaghetti Monster: ...

  9. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...advantage of this alternative is the possibility of real-time imaging. Robert Singer’s lab at Albert Einstein ...tag after pulse labeling of a determined period of time. Thus, only newly synthesized proteins of interest...

  10. RNA Extraction Without A Kit

    Type
    Blog Post
    ...isolating total RNA, this method requires a lot of time and depending on how many samples you may have, ...isopropanol precipitated. This method reduced the time it took to isolate RNA from 20+ hours to around ...

  11. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part...4.9 - 9 SEP 488* nm 508 nm ~6.5 - 9 *Optimal sequential excitations wavelengths as determined...

  13. To Each HIS Own

    Type
    Blog Post
    ...the activity or function of a single protein? Oftentimes, they rely on protein purification. In this strategy...to the metal ions once the protein is folded. Sometimes a tag placed on one end will be blocked during...

  14. All in a Twist: dsRNA

    Type
    Blog Post
    ...have been harnessing the power of dsRNA for a long time, even before we knew about all the diverse in vitro...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...

  15. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...has thus benefitted from countless rounds of optimization. Dozens of variants exist with unique cutting...and diversify PAM sequences of Cas12a to further optimize it for therapeutic and laboratory usage. Fast...

  16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...usually 1-3 of the first 3 bps are modified) for optimal stability and efficacy.  Base modifications What...mechanism to turn cutting off or on at a specific time. Recent advances in gRNA modification have overcome...

  17. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...“Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology by integrating... one thing, cells and cell lines degenerate over time—and sex can be hard to determine. To remedy this...

  18. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...incoming plasmids are sequenced at least twice, and sometimes more depending on the number of features we need...sequencing reactions. In the more complex cases, we sometimes have to design new primers to sequence the important...

  20. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. Selection...expressed only in specified cells or at specified times. Combining this with some of the loxP methods described...

  21. Working with Nuclear Receptors

    Type
    Blog Post
    ...be required by one or more NRs for efficient and timely activation or repression of gene expression (see...the research literature and acquiring them in a timely manner. Addgene represents an excellent solution...

  23. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...not only highlighted new tools but also included time for questions and a group discussion on the best... to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In 2018, the lab...

  24. Engaging with science and society at pgEd

    Type
    Blog Post
    ...for a year, I’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a...courses in social, health or policy-related topics, as time permits. Also seek out opportunities to communicate...

  25. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...interview is complete. Remember, your interviewers took time out of their day to talk with you, so be engaged...

  29. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...for targeting thousands of long ncRNAs at the same time (Liu et al. 2018). However, removing large lncRNA... the ncRNA (Figure 1D). While this method can be time-consuming, it is relatively effective in reducing...

  30. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...
Showing: 631 - 660 of 925 results