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  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability and yield. If a plasmid...using older plates could increase plasmid loss or mutation. Let it incubate Increasing the incubation time...

  2. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...for CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying to find local...informal science educators, and ever since, the State Museum has been the best supporter and most productive...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...reflected light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes...takes to image through a large z-stack (8).  One limitation of LSFMs using single side illumination, especially...

  4. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

  6. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...were more prohibitive at the time. With these limitations no longer an issue, bacterial in vivo cloning... N-terminus of a protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag...

  8. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13(7), 1288. https://... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

  9. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...Fixation allows you to freeze your samples in a given state or point in time. You want to halt degradative processes...methanol, or ethanol, work by dehydrating and precipitating cellular components. This method of fixation...

  11. CRISPR Antimicrobials

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    Blog Post
    ...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...floating on the water surface. The latter reflects a state of behavioral despair, and the more time the animal...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...

  13. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...cleaves a substrate, which will then form a blue precipitate. GUS is usually the first choice for establishing...transformation success. Luciferases are great quantitative reporters and use a substrate to produce bioluminescence...

  14. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...cell types (microglia are a notable exception) necessitates such molecular gymnastics for sensitive applications...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...: creativity. I’ve heard quite a few scientists state that they’re not creative, usually saying that they...your break can be as simple as a few minutes of meditation or a walk to the break room for a sip of water...

  18. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...

  19. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...amount of marker that is present, which would be annotated like so: CD14high or CD14low.  Don’t be intimated...liver and kidney recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...definitely glad we did - you can see some of the stats from the AMA to the right - the Reddit community...r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory questions...

  21. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...storage may be a bit dubious due to lack of documentation and high turn-over of lab personnel. If you ...These binding events can be impaired by the electrostatic repulsion of the negatively charged cell with...

  22. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... in the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...

  23. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...

  24. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...zoom on a camera lens without having to physically rotate the rings around the lens. It allowed me to focus... 4).  Learn: This works great for on-camera presentations and interviews. But how can I adapt this setup...

  26. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Medicago truncatula and several crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia...Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019)...

  27. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... signal from low abundance targets. Analysis/Presentation Once you’ve captured your images, various software...each cell. When making figures for a paper or presentation, make sure the images are accessible to all ...

  28. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...the transformed bacterial culture into the late stationary phase. Growing the culture at a lower temperature...low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103...

  30. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...gene is also useful in case the gene has a weird mutation that you didn’t know about (which could affect...theory you could use densitometric analysis to quantitate the intensity of the bands on your gel and get...
Showing: 631 - 660 of 836 results