We narrowed to 844 results for: Tat

...for CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying to find local...informal science educators, and ever since, the State Museum has been the best supporter and most productive...

...reflected light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes...takes to image through a large z-stack (8).  One limitation of LSFMs using single side illumination, especially...

...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

...the central dogma. We have the beginnings of computational logic and data storage. What we don't have are...begin to imagine designing 10-gene systems with quantitative precision. There are few designers of biological...

...were more prohibitive at the time. With these limitations no longer an issue, bacterial in vivo cloning... N-terminus of a protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag...

...studies (e.g. using mariner), as a method of tagmentation for next-gen sequencing pipelines (e.g. using...the mini-transposon can occur in two different orientations, but is strongly biased towards T-RL, where ...

...Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13(7), 1288. https://... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

...Fixation allows you to freeze your samples in a given state or point in time. You want to halt degradative processes...methanol, or ethanol, work by dehydrating and precipitating cellular components. This method of fixation...

...follows: (my_house + coffee) - starbucks See how the notation works? The + operator denotes intersection and...Through a bit of stochastic magic and Poisson statistics, each sub-population has, on average, one distinct...

...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

...Developing AAV enhancer tools for brain visualization Putative enhancer sequences for specific cell types can... GTA has a wealth of information and includes annotated images of the brain sections describing the labeled...

...geothermalis (Dge10TnpB) targets a different TAM (5′-TTAT-3′). The unique TAM requirements expand the number...al., 2020; Qiao et al., 2025). However, a key limitation of viral vectors is their restricted cargo capacity...

...floating on the water surface. The latter reflects a state of behavioral despair, and the more time the animal...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...

...cleaves a substrate, which will then form a blue precipitate. GUS is usually the first choice for establishing...transformation success. Luciferases are great quantitative reporters and use a substrate to produce bioluminescence...

...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...cell types (microglia are a notable exception) necessitates such molecular gymnastics for sensitive applications...

...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...

...were designed to target genomic sites with no annotated function, i.e. sites that lack open chromatin ...safe-targeting guides, which target genomic sites with no annotated function. In the growth screen, safe-targeting...

...: creativity. I’ve heard quite a few scientists state that they’re not creative, usually saying that they...your break can be as simple as a few minutes of meditation or a walk to the break room for a sip of water...

... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...

...amount of marker that is present, which would be annotated like so: CD14high or CD14low.  Don’t be intimated...liver and kidney recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23...

...definitely glad we did - you can see some of the stats from the AMA to the right - the Reddit community...r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory questions...

...storage may be a bit dubious due to lack of documentation and high turn-over of lab personnel. If you ...These binding events can be impaired by the electrostatic repulsion of the negatively charged cell with...

... in the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...

...Nature report from researchers at Amgen, it was stated that only 11% of the academic research in the literature...with them including reports and protocols. To facilitate this, some ELNs allow you to invite people and...

...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...

...zoom on a camera lens without having to physically rotate the rings around the lens. It allowed me to focus... 4).  Learn: This works great for on-camera presentations and interviews. But how can I adapt this setup...

...Medicago truncatula and several crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia...Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019)...

...AAV receptor (AAVR)2 Laminin receptor (LR)3 Putative integrin3 AAV11 Carboxypeptidase D (CPD).... (2006). Α2,3 and α2,6 N-Linked Sialic Acids Facilitate Efficient Binding and Transduction by Adeno-Associated...

... signal from low abundance targets. Analysis/Presentation Once you’ve captured your images, various software...each cell. When making figures for a paper or presentation, make sure the images are accessible to all ...

...the transformed bacterial culture into the late stationary phase. Growing the culture at a lower temperature...low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103...