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We narrowed to 1,025 results for: URE

Showing: 631 - 660 of 1025 results

  1. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...lucky enough to have an imaging core or tissue culture core, you are looking at a process-oriented team...come across one. Also, remember, one important feature that all projects have in common - and by association...is a blog post, so here are just a few critical features based on my own experience: Leadership Few things...certainly necessary, but equally important is making sure all team members understand what roles they’re meant...well, you can learn from the experience and make sure your next team works better. In fact, don’t wait...

  2. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... of a scientist was like. The word “scientist” conjured up images of men like Albert Einstein and Bill... make decisions about what we want to do in the future. How do I get started with research? I had no idea...personal interests While it’s perfectly fine to be unsure, it helps to have a vague sense of what kind of..., even if a question is a very basic one, making sure that you have a good fundamental understanding of...goals helped me reflect on what I wanted for my own future. Informational interviews with people who have ...

  3. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...breakthroughs and undermine critical research infrastructure that has taken decades to establish.These funding...materials that might otherwise be lost amid lab closures or project terminations, ensuring that scientific...grant applications, addressing the new funding structures and indirect cost caps more efficiently. Offering...can navigate this difficult period together. The future of scientific progress depends on our collective...

  4. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...screenshot below to see what I mean. Additional features Once you've clicked on the "# References" link...article abstract and more. We've also included a feature to help save you from performing any unnecessary...domains" publication.  If you want to see this new feature in action, click here to explore the TetO-FUW-OKSM...

  5. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...Doudna and Eva Nogales presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography...Cas9's structural details. "Understanding this structure may help us engineer around the current limitations...in Feng Zhang's lab, in a press release. "In the future, it could allow us to design versions of these ...University summed it up well in that New York Times feature when he said, “All of this has basically happened...

  7. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ... useful tools because we can easily detect and measure the light they emit. Proteins that give off light...observed in the crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the...this new green glowing protein could be used to measure gene transcription, as well as to track protein...GFP’s function. His lab described the protein’s structure and also discovered many mutations that alter ...

  8. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ... a dish that features that ingredient. We just had a cook-off last week, and the featured ingredient was...Harry Potter-style.The shared lunches create a culture of openness and trust that comes from caring about...ll dedicate a post to these competitions in the future, and hopefully include some of our favorite winning...

  9. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...values with each element. We also set out to make sure the page looked clean, professional, and modern....our website, or in social media, we wanted to make sure our friend matched our core branding. Blugene needs... our values of community and sharing.  One big feature upgrade? Blugene now has a full expression. You...Addgene's new search tool Read more about other features of Addgene's website Resources on Addgene.org...

  10. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...heart health. These are amazing advances that will surely save thousands if not millions of lives, but many...resources and promoting research for rare disease cures. Through their BeHEARD Award, the Rare Genomics ...better choose which genes and pathways to target in future small molecule screens. He plans to work with a...voice. Check back with Addgene often to learn of future rare disease research funding opportunities. References...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ... visualization. However, due to the nature of the size, structure, and other qualities of each tag class...individually isolate a protein, there must be a special feature about that protein that no other protein in the...for an extra clean up step of the tag – a handy feature. If you decide not to cleave the GST tag after/...

  12. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ... lunch. There was something so special about her pure interest and curiosity to understand my work and...making of this photo!  Here, I was also given a mixture of support and independence that allowed me to ...on content strategy. I really believe that the culture at Addgene cultivates trust between coworkers and... more confident in myself, my abilities, and my future - something I didn’t expect from a co-op experience...

  13. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...for helpful information on the known features and relevant literature, or to decide which line is best for...cell line collections websites and catalogues Literature searches utilizing Google Scholar alerts and ...as commonly misidentified lines. Browsing this feature to know if your lines may be especially vulnerable...

  14. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...That includes keeping fees as low as possible to ensure that our materials are affordable but enough to...community-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better job of... our core values. These accurately reflect our culture and outlook: Addgene increases productivity by...increase sharing in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes...

  15. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ... first need to decide the best way to track or measure the process you’re interested in. Depending on ...examine, i.e. for an enzymatic reaction, you could measure consumption of a substrate OR production of a product...molecule biosensors Addgene has many sensors that measure the concentration of small molecules like arabinose...withstand a particular solute concentration or temperature? For further advice, see our posts on how to ...

  16. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...Rodríguez et al., 2011).  Genetics of SPARC Figure 1: SPARC and SPARC2 use the GAL4-UAS system along...sparse (S) labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) ...gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/...

  17. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...boxes). We get these boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will...company and it has to be packaging (no coated tissue culture plates, for example). Check the link for more details...or Axygen®, set up a bin or box in your tissue culture room for the wrappers and ship them back. Start...make a huge impact directly on waste, but also on culture. Encourage your workplace to become a leader in...

  18. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...Ashley Waldron Organoids are three-dimensional cultures that mimic aspects of organ morphology and physiology... an organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag ...targeted cells that can be used to generate organoid cultures with a modification of interest. While the authors...other Cas12a nucleases identified, functions at temperatures up to 43 °C and can be used for genome editing...

  19. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...wanted to be. But prior to graduate school, I wasn’t sure a master’s was a good decision. So how do you know...means to gain employment, so it’s important to make sure a masters helps you get a job you want. But how ...with master's have Job descriptions often fail to capture all of the responsibilities of a role, so it helps...questions in this blog post can help you start to figure out if a master’s is a good fit with your interests...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes the... the method is powerful and offers immediate measurements in a live animal that can be correlated to behavior...developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian...powerful new developments in the detection and measurement of neurotransmitters in various species and while...

  21. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...very painful FOP flare ups. Eventually, without a cure, AJ will begin losing mobility and not be able to...play like the other kids his age. A potential FOP cure But, there is HOPE. FOP is a genetic disease typically...solutions to treat rare diseases like FOP until a cure is available. Addgene and AJ's Avengers This year...knowledge of FOP, it’s biological causes, and potential cures. We encourage any researchers working on ACVR1 or...

  22. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...young scholars across Europe get introduced to the cultures and opportunities available to them at university...to students. Freshers fairs are also where many future scientists are introduced to the biology and chemistry...talks by prominent scientists, including Nobel Laureates, who speak about their work and current, trending...positions both inside and outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to...

  23. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...readthrough mitigation. Nature. (2016) 534, 719-723. DOI: 10.1038/nature18308   Additional resources on...are unreliable for a variety of reasons (Baker, Nature). Tagging your protein allows you access to a ...Reproducibility crisis: Blame it on the antibodies. Nature (2015) 521, 274-276 DOI: 10.1038/521274a Arribere...

  24. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ... both targets in your specimen. You scour the literature and find an ideal antibody against Protein A ...distinguish between your two targets.    Figure 1: In the isotype conversion process the variable...successfully visualize both proteins.     Figure 2: Two primary antibodies with the same isotype... the anti-Protein B antibody (Fig. 3)    Figure 3: Recommended secondary antibodies for rat IgG1...

  25. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...synthesis. They published their results in a paper in Nature Communications announcing a new bioinformatics ...good predictors because they are just that: rare. “Nature has many genes where there are not rare codons ...source genes from farther away in the tree of life." Nature communications 9.1 (2018): 4425. PubMed PMID: 30356044...to predict the lab-of-origin of engineered DNA." Nature communications 9.1 (2018): 3135. PubMed PMID: 30087331...

  26. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...Updated April 21, 2020 Picture this: you’re a busy scientist anxiously awaiting the arrival of the newest...signs each agreement just once, covering nearly all future requests/deposits. This reduces the processing ...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material transfer...

  27. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...selection-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so successful... sequences are inserted into the genome. Figure 1: Self-excising cassette schematic from Dickinson...efficient for C. elegans genome modification. Figure 2: Schematic from Dickinson et al. (2015) illustrating...may prove effective in other species or in cell culture. In addition to CRISPR/Cas9 applications, SEC selection...

  28. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ... possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch sensor...by a secondary screen in rat hippocampal neuron cultures. The optimized Twitch sensors allowed the researchers...toxic,” Merkx explained. New tools were needed to measure them. Merkx says, while in principle adapting a...them,” Merkx said. “For us, it is one of the main measures of success – whether we have really developed ...

  29. The MTA at Addgene

    Type
    Blog Post
    ...use agreements like MTAs.  Addgene does a lot to ensure that we can facilitate a fast and agile MTA process...material under the specified terms. This acts as the signature on any subsequent MTA for that plasmid facilitated... handled the majority of our MTA requests and signatures via an online process, though the process has...materials are requested, instead of coordinating signatures from two different institutes. Our online portal...

  30. Science Career Options

    Type
    Blog Post
    ...problem or complete a project, overcoming inevitable failures and setbacks. There are many roles that actively...because they enjoy spending time outdoors and with nature. Experience in the field or in a position that ...Conservationist Documentary Director Parasitology Botanist Nature Photographer/Artist Ecologist     Computer...Include any relevant job specific ‘buzz words’ to ensure recruitment agencies and employers put your resume...
Showing: 631 - 660 of 1025 results