We narrowed to 1,104 results for: Ide

... founding Addgene, where the idea for Addgene came from and how the idea got off the ground Chapter 3 ...the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's...

... represents the continuation of our efforts to provide resources to researchers in developing countries...Ghanaian women by including PCR screening for HR HPV alongside the cytological tests currently in use. Gladys...screens. She hopes that these screens will both identify individuals who are infected with HR HPV and therefore...

...share scientific discoveries and developments, and provide tips and tricks for new and seasoned molecular ...specific area of interest. We also cover topics outside of these five areas and you can find what you need...comments below. We’ll track down the story, get the inside scoop, and keep you up-to-date. Be the first to...

...Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding reagents. In the ...and expanded. The clonal cells are screened to identify hybridoma lines, each producing a monoclonal antibody...ensuring affordable access to materials, repositories provide standardized quality-control and technical support...

...-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs). Sakuma said that the new system could...likely to work in animal embryos based on earlier evidence showing that a CRISPR/Cas9 plasmid could be injected...vector-mediated multiplex genome engineering. The system now provides an efficient targeting strategy for multiplex ...

...citation. If you’re considering requesting an Addgene antibody, the Data Hub can help you decide if an antibody... your application. It can be quite difficult to decide on which antibody to request for your experiment...

...student in the Collins' lab at the time) got the idea to engineer bacteriophage (viruses that infect bacteria...bacteria) for use as antibiotics. To develop this idea, Drs. Koeris and Lu founded the biotech startup ... as antibiotics 8:26 - 10:30 : How Dr. Koeris' decided to develop phage for use in a biotech startup 12...

...visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies represent...travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel. Bluegene...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from Our Bloggers...

...the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and... might suspect contamination in your sample or consider asking your sequencing facility to apply a special...vendors. The precipitation method has an unfortunate side effect of messing up the reaction around base 70...

...committee creates a compensation philosophy that helps guide employee compensation and also serves as an objective...think is the most important resource that Addgene provides the scientific community? Access to plasmids which...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

...Stanford University). Right there on the floor outside the lecture hall. It was wonderful to see the ad-hoc...acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows...Microbial World’ showcased complex connections and ideas in various visualized approaches from hand-drawn...

...on the Addgene blog and have created and updated guides on various topics in biology over the past year...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and...Cas9 and reverse transcriptase and a prime editing guide RNA, it can introduce insertions, deletions, and...

...Pooran surveyed a CRISPR google forum to get a better idea of how many people are having trouble, to determine...from Cornell University, is a bit old school and provides a thorough walkthrough of how you can use PCR ...Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science Talk Developing Lab...

...high-throughput manner, but these systems have not been widely employed in vivo. The CHIME and new X-CHIME systems...deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene knockout in immune systems...CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in Mammalian...

...treatment, it is best to identify a cocktail of 3-12 phages. Once these phages are identified, they must then ... forward to help, the Phage Directory team must decide which labs should receive the clinical sample to...

...may have only 2-3 formal supervisors that will provide guidance and experience. Navigating a fulfilling...about mentoring In this blog series, I plan to provide answers to the top questions scientists ask me ...teaches you to fish, doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them...

...Protein Collection webpage. These plasmids are divided into the following categories: Over 300 empty ...localizes in the cell, use these markers as your guide. As Michael Davidson told the Tallahassee Democrat...go together, we can fix it. Right now, we have no idea." Now, it will be that much easier for scientists...

...viewing sequencing traces, while the paid version provides more tools for plasmid mapping and design (Figure... deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database...molecular biology protocols Browse our protocol video collection ...

...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally...

...fact, there was so much useful information that I decided to write a few blog posts. This first post will...the box so the pre-paid postage stamp is on the outside of the container, and drop off the box at any location...sustainability leaders. Take initiative to bring ideas to your workplace and you could end up making a ...

...Publishing Institute (SWPI) is an extremely beneficial guide to becoming a successful writer. When I originally...course The online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy... in Washington D.C. at the ASM headquarters. I decided to go ahead and apply; thinking I probably would...

...reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound. Challenges...experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration and... imaging. Specifically, arg1 expressing E. coli aided in ultrasound imaging of the mouse gastrointestinal...

...joined Sanofi/Genzyme nearly three years ago I decided it was time to get in shape. After three kids, ... to raising funds and awareness of FOP. Please consider running with us or making a donation via the link... who is a big superhero fan. This year, I have decided to run as The Green Lantern’s friend, St Walker...

... how microbiology research is improving lives worldwide and the presentation made it clear how important...up with former colleagues! I ran into past ASM President Tim Donohue (see below) on my way back from getting... with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth. As well as being the Director...

...Integrated DNA Technologies (IDT). “It was fairly accidental. I was finishing graduate school, and didn’t ...these days. I do some public speaking and a bit of video work for webinars (get tips for your next science...bring people in.” Are you interested in careers outside academia like science communication? If so, let...

...results. GeoCas9 RNP editing in mammalian cells Besides being able to take some heat, GeoCas9 can introduce...HEK293 cells with GeoCas9 or SpCas9 RNP’s and a guide targeting a site with an overlapping PAM. In both...on Your CRISPR Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...

...conversations. This creates a level of familiarity and confidentiality to ensure more honest answers. Ask people who...resources to help you choose a good mentor, I have provided a downloadable PDF at the end of this blog post... may hedge on their answers to your questions. Consider reading Toxic Academic Mentors by @drmellivora...

...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...viruses later on. These built-in safety features provide extra layers of risk management when working with...short-term? Answering these questions will help guide you to the right method for your experiment.  A ...

...of inquiry with which I was not yet familiar. The idea that my piecemeal degree might one day foster a ... several projects nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics... appeal to a given audience and how to utilize a wide array of media in doing so—can prove invaluable ...

...methods for data analysis, our team’s goal is to provide scientists with new tools and protocols that they...labs looking to improve their AAV yields. Given the wide range of downstream applications scientists rely...resources of the Addgene blog Read our beginner's guide to viral vectors Find all of our viral vector blog...

....  These days commercially available TOPO kits provide vectors or cloning arms with overhanging 3´ deoxythymidine... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....Molecular Biology Techniques Watch Our Protocol Videos ...

...cellular-level events and behavior. It’s a problem that he considers “one of the greatest challenges of neuroscience...particular advantage for observing events taking place inside cells that are on the move, such as T lymphocytes...protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had a high-affinity...

...brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a toolset that... have tried to make green molecules, but then alongside blue they actually have a lot of crosstalk. So...for the first time? Boyden: There are always considerations for each species. This could be a three or ...

...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...disease models established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

...used to infer differentiation trajectories and identify both up- and down-regulated pathways. Individual...unique barcodes for each TF help streamline identification. While the Zhang lab controlled their multiplicity... a model system. TF screens can be designed to identify custom TF programs to acutely initiate disease...

...CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target the genome...applications when repetitive tRNA sequences must be avoided, different tRNAs with optimized 5’ leader sequences...array for multiplex gene editing applications, we provide detailed manual and online design examples of using...

...coinjection into zygotes of Cas9 mRNA and a single-guide RNA targeting the mutant allele (7). An independent...CRISPR/Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently...These papers suggest it might work well enough to consider as a therapeutic.” There are many fundamental ...

...the pMinDisplay backbone is fused to a specific peptide segment (PDGFR TMD) that anchors the sensor to ...the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD, the researchers...ordered directly from Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems you ...

...that interact with the virus. As a result, we identified 332 human proteins that the virus interacts with...the help of human proteins in their lifecycle, identifying these potential human helpers of SARS-CoV-2 (...You could be the next scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected...

...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...