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We narrowed to 838 results for: ANG

Showing: 421 - 440 of 838 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...AAV-containing 40% fraction is collected and buffer exchanged to remove the iodixanol and concentrate the purified...or the virus can be stored at 4 °C and buffer exchanged the next day. Refer to the AAV Iodixanol Gradient...correctly. When aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots...

  2. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed... NgAgo/ssDNA requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells...website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for...

  4. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...speakers each week, my department and graduate office arranged opportunities to meet with various professionals...volunteering, I was worried about becoming one of those angry, disappointed graduate students because I wasn’t...place to go once a week to get recharged. The fun change of pace helped break up the negativity and allowed...

  5. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...through wild populations. Whereas most genomic changes impose a fitness cost and are eliminated by natural...previous gene drive and thereby undo the genetic changes driven by the earlier intervention2. While an initial.... An accidental release would be particularly dangerous for molecular biology and genetics because the...

  6. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...a characteristic absorption spectra across this range of wavelengths with maximal absorption at 260 nm...present in the sample also absorb light in this range of wavelengths (Figure 1B-D). These other molecules...Proteins absorb light to varying degrees across the UV range used in NanoDrop systems. Peak absorption occurs...

  7. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... colonies are an issue. Pros: Very stable, interchangeable with Amp, timesaving for transformations. Con...similar relationship as amp and carb (they are interchangeable). Like carb, spectinomycin is the more stable..." %}   References and Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley,...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... VSV-G envelope have high infectivity in a wide range of cell types, so they’re ideal for work with primary...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID...β-thalassaemia. Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, ...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...for later culture: At this point, you will want to hang onto your clones for later use. There are a few ...directionality. Verifying the insert sequence with Sanger Sequencing After identifying a few positive clones...mini-prep these clones and submit the plasmids for Sanger sequencing. Sequencing allows you to confirm the...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    ...quinolone resistance, mediated by a single base pair change in DNA gyrase, CRISPR phagemids were cytotoxic ...diverse, and each phage has a narrow bacterial host range. To further potential clinical applications, Ando...Beisel, Chase L., Ahmed A. Gomaa, and Rodolphe Barrangou. “A CRISPR design for next-generation antimicrobials...

  11. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...dressing in costume, and holding competitions (ranging from cook-offs, to corn toss to trivia, and everything...participation numbers for an event you are planning, dangle the carrot (or more popularly- cookies). 3. Do...quiet guy from the lab was such a master drywall hanger?  4. Utilize your office space too Balance point...

  12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...Addgene realizes the One-Stop shopping for a wide range of plasmids. The merit of depositing plasmids to... which is very disappointing. I hope I can help change the situation in the future. Addgene: What advice... they may be able to achieve something that may change the rules of the game. In this regard, I believe...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...physiological processes. Re-engineering GFP to increase its range of color and application Almost as soon as its...domains of a protein that undergoes conformational change. Typically, two fluorescent proteins with overlapping...ion (such as Ca2+) concentrations and pH, using a range of strategies such as FRET, calmodulin, and others...

  14. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...lentiviral and retroviral packaging vectors are not interchangeable. While these differences may seem subtle they...the generation used to package the virus does not change the generation of the transfer vector. Q5: What...virus.  VSV-G is very common due to its broad host range, and increased particle stability; however, the ...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, A. R. (...functional properties, and is amenable to a wide range of biophysical assays (Kroupova et al., 2024). They...

  16. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes conjugated with its HaloTag...Through in vivo screening of human promoters, the Zhang Lab found a short fragment of human IBA1 promoter...functionally reconstituting coding sequences for a range of applications, including CRISPR activation by ...

  17. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Jhunjhunwala, S., Aresta-Dasilva, S., Tang, K., Alvarez, D., Webber, M., Tang, B., Lavin, D., Veiseh, O., Doloff...Vegas, A., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    ...structures and compartments in mammalian cells By Angela Abitua Genetically encoded fluorescent proteins...system for human pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation...s41598-020-57500-1 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...

  19. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...temperature parameters. For example, we found that changing the annealing temperature of the PCR from 60° ...primers can have different annealing efficiencies (Wang et al., 2013). Although you can optimize your primers...: 28546538. PubMed Central PMCID: PMC5445070. 6. Wang, Feng, et al. "A reliable and feasible qPCR strategy...

  20. How to Write a Scientific Review Article

    Type
    Blog Post
    ...popular papers, but looked for papers from a wide range of authors. Just start writing When I first started... and forth with. That being said, we probably exchanged dozens of drafts of the manuscript. This is the...transform the document into something cohesive-- change the sentences, make it flow, and start telling ...
Showing: 421 - 440 of 838 results