We narrowed to 492 results for: ARA-2

... the Cas machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA...from work being done today, is a nearly entirely separate effort from acknowledging its existence. Studying...

... replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through insertional... are technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...

... more from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression...adapted from Farahani et al. 2022, under CC-BY license.   Find pYtags plasmids here!   Farahani PE, et al...for monitoring specific RTK activation in vivo (Farahani et al. 2022). The authors take advantage of the...better response kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and...   Matlashov ME, et al. Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium...for lactate developed using a high-throughput multiparameter screen (Koveal et al. 2022). Lactate is a key... here!   Koveal D, et al. A high-throughput multiparameter screen for accelerated development and optimization...

..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...targeted to the endoplasmid reticulum based on the appearance of OSER whorls. This assay is quantitative and...also collected data on fluorescent protein characteristics such as the brightness, photostability, maturation...Cranfill et al, 2016; Lambert, 2019). These characteristics are important, but have less priority than ...microscopy (University of Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes...PMC4383620. Botman, Dennis, et al. "In vivo characterisation of fluorescent proteins in budding yeast."...brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM, for ratiometry...

...Nature 339 (1989). 487–488. PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward... storage buffers used, and other experimental parameters. For example, while freeze thaw is generally ...25073060. 8. Finkelshtein, D., Werman, A., Novick, D., Barak, S., Rubinstein, M. “LDL receptor and its family...

... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...and virtually never exceeds 40%. While this is comparable or better than the efficiency of most current...ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This delivery method frees...

...rules are: (1) don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same...highest-fidelity overhang set it can find for those parameters. For even more specificity, you can exclude specific...

...systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created with...been challenging. It can be difficult to ensure comparable expression of all components of Cascade for maximum...

...that view (Callahan et al., 2022).     Fig. 2: Students in an MDH CURE replaced select residues ...Part I – guided inquiry – purification and characterization of a fusion protein: Histidine tag, malate...

...interesting things to do, feel free to crowdsource! 2. Free food generally gets people to show up If you... company culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently...

...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...seekers to use online applications with significant character limits for the written portion of the grant application...

...externally fertilized, transparent progeny (Figure 2). These features give it a leg up (or a fin up?) over...time and space, and was also used to precisely characterize developmental defects in the one-eyed pinhead...

...based antiviral method to degrade RNA from SARS-CoV-2 sequences and live influenza A virus. It was demonstrated...stuck in inclusion bodies. Takayuki Iwaki, Kimiko Hara, and Kazuo Umemura have described a simple method...

...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE...and Discovery As part of their quest to find, characterize, and develop new CRISPR systems and technologies...

...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation...tested their expression under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression...escape). The rhamnose induction system is well-characterized and quite tight (Giacalone et al., 2006). The...though Khetrapal et al. also demonstrated that the arabinose promoter worked too. Rhamnose induction relies...References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled...

... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...and multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared... found to be a dimer3,15. ND-not determined in parallel assays with other FPs.   We hope that this post...

...can still record the signal separately for each target.   Figure 2: Examples of simultaneous multiplex...or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples...samples, or other preparations. Sequential IF For a sequential IF experiment (Figure 1A), you’ll block, add...different color fluorophore that you can image separately on your microscope. Abcam's sample protocol is...different antibodies, blocking, washing, and sample preparation steps, in order to ensure your antibodies are...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

...variety of environmental conditions, and double every 2 hours. Yeast are also the first eukaryotic genome ...as Parkinson’s disease (PD). PD is primarily characterized by protein misfolding that leads to a build-...23594743. PubMed Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology & medicine...

...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...favorite books, Michael Ende’s Momo, the title character endears herself to the nearby village (and to ...or how completely different teams are tackling comparable conflicts. Is this level of domain knowledge ...

...There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells...proteins can then be purified using streptavidin and separated via flow cytometry.  Find plasmids for EXCELL ...